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Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols
INTRODUCTION: Herein, the authors describe a simple enhancement to a commercial rapid DNA extraction kit based on simple viral lysis for detecting COVID-19 via RT-qPCR. METHODS: After testing several different modifications, the adapted protocol with the best results in preliminary experiments was s...
Autores principales: | Pessoa-e-Silva, Rômulo, de Oliveira, Priscilla Stela Santana, Gonçalves, Sayonara Maria Calado, Guarines, Klarissa Miranda, Carvalho, Lidiane Vasconcelos do Nascimento, Correia, Maria Andreza Bezerra, da Rosa, Michelle Melgarejo, Rêgo, Moacyr Jesus Barreto de Melo, Pitta, Maira Galdino da Rocha, Pereira, Michelly Cristiny |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Medicina Tropical - SBMT
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582969/ https://www.ncbi.nlm.nih.gov/pubmed/34787260 http://dx.doi.org/10.1590/0037-8682-0270-2021 |
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