Prion protein polymorphisms in Michigan white-tailed deer (Odocoileus virginianus)

Chronic Wasting Disease (CWD), a well-described transmissible spongiform encephalopathy of the Cervidae family, is associated with the aggregation of an abnormal isoform (PrP(CWD)) of the naturally occurring host prion protein (PrP(C)). Variations in the PrP gene (PRNP) have been associated with CWD...

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Autores principales: Ott-Conn, Caitlin N., Blanchong, Julie A., Larson, Wes A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583003/
https://www.ncbi.nlm.nih.gov/pubmed/34751633
http://dx.doi.org/10.1080/19336896.2021.1990628
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author Ott-Conn, Caitlin N.
Blanchong, Julie A.
Larson, Wes A.
author_facet Ott-Conn, Caitlin N.
Blanchong, Julie A.
Larson, Wes A.
author_sort Ott-Conn, Caitlin N.
collection PubMed
description Chronic Wasting Disease (CWD), a well-described transmissible spongiform encephalopathy of the Cervidae family, is associated with the aggregation of an abnormal isoform (PrP(CWD)) of the naturally occurring host prion protein (PrP(C)). Variations in the PrP gene (PRNP) have been associated with CWD rate of infection and disease progression. We analysed 568 free-ranging white-tailed deer (Odocoileus virginianus) from 9 CWD-positive Michigan counties for PRNP polymorphisms. Sampling included 185 CWD-positive, 332 CWD non-detected, and an additional 51 CWD non-detected paired to CWD-positives by sex, age, and harvest location. We found 12 polymorphic sites of which 5 were non-synonymous and resulted in a change in amino acid composition. Thirteen haplotypes were predicted, of which 11 have previously been described. Using logistic regression, consistent with other studies, we found haplotypes C (OR = 0.488, 95% CI = 0.321–0.730, P < 0.001) and F (OR = 0.122, 95% CI = 0.007–0.612, P < 0.05) and diplotype BC (OR = 0.340, 95% CI = 0.154–0.709, P < 0.01) were less likely to be found in deer infected with CWD. As has also been documented in other studies, the presence of a serine at amino acid 96 was less likely to be found in deer infected with CWD (P < 0.001, OR = 0.360 and 95% CI = 0.227–0.556). Identification of PRNP polymorphisms associated with reduced vulnerability to CWD in Michigan deer and their spatial distribution can help managers design surveillance programmesand identify and prioritize areas for CWD management.
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spelling pubmed-85830032021-11-12 Prion protein polymorphisms in Michigan white-tailed deer (Odocoileus virginianus) Ott-Conn, Caitlin N. Blanchong, Julie A. Larson, Wes A. Prion Research Paper Chronic Wasting Disease (CWD), a well-described transmissible spongiform encephalopathy of the Cervidae family, is associated with the aggregation of an abnormal isoform (PrP(CWD)) of the naturally occurring host prion protein (PrP(C)). Variations in the PrP gene (PRNP) have been associated with CWD rate of infection and disease progression. We analysed 568 free-ranging white-tailed deer (Odocoileus virginianus) from 9 CWD-positive Michigan counties for PRNP polymorphisms. Sampling included 185 CWD-positive, 332 CWD non-detected, and an additional 51 CWD non-detected paired to CWD-positives by sex, age, and harvest location. We found 12 polymorphic sites of which 5 were non-synonymous and resulted in a change in amino acid composition. Thirteen haplotypes were predicted, of which 11 have previously been described. Using logistic regression, consistent with other studies, we found haplotypes C (OR = 0.488, 95% CI = 0.321–0.730, P < 0.001) and F (OR = 0.122, 95% CI = 0.007–0.612, P < 0.05) and diplotype BC (OR = 0.340, 95% CI = 0.154–0.709, P < 0.01) were less likely to be found in deer infected with CWD. As has also been documented in other studies, the presence of a serine at amino acid 96 was less likely to be found in deer infected with CWD (P < 0.001, OR = 0.360 and 95% CI = 0.227–0.556). Identification of PRNP polymorphisms associated with reduced vulnerability to CWD in Michigan deer and their spatial distribution can help managers design surveillance programmesand identify and prioritize areas for CWD management. Taylor & Francis 2021-11-09 /pmc/articles/PMC8583003/ /pubmed/34751633 http://dx.doi.org/10.1080/19336896.2021.1990628 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Ott-Conn, Caitlin N.
Blanchong, Julie A.
Larson, Wes A.
Prion protein polymorphisms in Michigan white-tailed deer (Odocoileus virginianus)
title Prion protein polymorphisms in Michigan white-tailed deer (Odocoileus virginianus)
title_full Prion protein polymorphisms in Michigan white-tailed deer (Odocoileus virginianus)
title_fullStr Prion protein polymorphisms in Michigan white-tailed deer (Odocoileus virginianus)
title_full_unstemmed Prion protein polymorphisms in Michigan white-tailed deer (Odocoileus virginianus)
title_short Prion protein polymorphisms in Michigan white-tailed deer (Odocoileus virginianus)
title_sort prion protein polymorphisms in michigan white-tailed deer (odocoileus virginianus)
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583003/
https://www.ncbi.nlm.nih.gov/pubmed/34751633
http://dx.doi.org/10.1080/19336896.2021.1990628
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