Cargando…
E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis
Escherichia coli Nissle (EcN), a probiotic bacterium, has been employed in treating inflammatory bowel disease, but the nature of its therapeutic effect is not fully understood. Intestinal inflammation alters the environment, exposing the microbial population to new stresses and eliciting transcript...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583297/ https://www.ncbi.nlm.nih.gov/pubmed/34751631 http://dx.doi.org/10.1080/19490976.2021.1994832 |
_version_ | 1784597172228980736 |
---|---|
author | Dacquay, Louis C Tsang, Derek Chan, Donny Parkinson, John Philpott, Dana J McMillen, David R |
author_facet | Dacquay, Louis C Tsang, Derek Chan, Donny Parkinson, John Philpott, Dana J McMillen, David R |
author_sort | Dacquay, Louis C |
collection | PubMed |
description | Escherichia coli Nissle (EcN), a probiotic bacterium, has been employed in treating inflammatory bowel disease, but the nature of its therapeutic effect is not fully understood. Intestinal inflammation alters the environment, exposing the microbial population to new stresses and eliciting transcriptional responses. We administered EcN to germ-free mice and then compared its transcriptional response between DSS-treated and untreated conditions using RNA-seq analysis to identify 187 differentially expressed genes (119 upregulated, 68 downregulated) and verifying a subset with qRT-PCR. The upregulated genes included many involved in flagella biosynthesis and motility, as well as several members of the formate hydrogenlyase complex. Despite prior evidence that these pathways are both transcriptionally regulated by nitric oxide, in vitro tests did not establish that nitric oxide exposure alone elicited the transcriptional response. The results provide new information on the transcriptional response of EcN to inflammation and establish a basis for further investigation of its anti-inflammatory activity. |
format | Online Article Text |
id | pubmed-8583297 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-85832972021-11-12 E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis Dacquay, Louis C Tsang, Derek Chan, Donny Parkinson, John Philpott, Dana J McMillen, David R Gut Microbes Brief Report Escherichia coli Nissle (EcN), a probiotic bacterium, has been employed in treating inflammatory bowel disease, but the nature of its therapeutic effect is not fully understood. Intestinal inflammation alters the environment, exposing the microbial population to new stresses and eliciting transcriptional responses. We administered EcN to germ-free mice and then compared its transcriptional response between DSS-treated and untreated conditions using RNA-seq analysis to identify 187 differentially expressed genes (119 upregulated, 68 downregulated) and verifying a subset with qRT-PCR. The upregulated genes included many involved in flagella biosynthesis and motility, as well as several members of the formate hydrogenlyase complex. Despite prior evidence that these pathways are both transcriptionally regulated by nitric oxide, in vitro tests did not establish that nitric oxide exposure alone elicited the transcriptional response. The results provide new information on the transcriptional response of EcN to inflammation and establish a basis for further investigation of its anti-inflammatory activity. Taylor & Francis 2021-11-09 /pmc/articles/PMC8583297/ /pubmed/34751631 http://dx.doi.org/10.1080/19490976.2021.1994832 Text en © 2021 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Dacquay, Louis C Tsang, Derek Chan, Donny Parkinson, John Philpott, Dana J McMillen, David R E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis |
title | E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis |
title_full | E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis |
title_fullStr | E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis |
title_full_unstemmed | E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis |
title_short | E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis |
title_sort | e.coli nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583297/ https://www.ncbi.nlm.nih.gov/pubmed/34751631 http://dx.doi.org/10.1080/19490976.2021.1994832 |
work_keys_str_mv | AT dacquaylouisc ecolinissleincreasestranscriptionofflagellaassemblyandformatehydrogenlyasegenesinresponsetocolitis AT tsangderek ecolinissleincreasestranscriptionofflagellaassemblyandformatehydrogenlyasegenesinresponsetocolitis AT chandonny ecolinissleincreasestranscriptionofflagellaassemblyandformatehydrogenlyasegenesinresponsetocolitis AT parkinsonjohn ecolinissleincreasestranscriptionofflagellaassemblyandformatehydrogenlyasegenesinresponsetocolitis AT philpottdanaj ecolinissleincreasestranscriptionofflagellaassemblyandformatehydrogenlyasegenesinresponsetocolitis AT mcmillendavidr ecolinissleincreasestranscriptionofflagellaassemblyandformatehydrogenlyasegenesinresponsetocolitis |