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The Great Escape: mRNA Export through the Nuclear Pore Complex

Nuclear export of messenger RNA (mRNA) through the nuclear pore complex (NPC) is an indispensable step to ensure protein translation in the cytoplasm of eukaryotic cells. mRNA is not translocated on its own, but it forms ribonuclear particles (mRNPs) in association with proteins that are crucial for...

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Autor principal: De Magistris, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583845/
https://www.ncbi.nlm.nih.gov/pubmed/34769195
http://dx.doi.org/10.3390/ijms222111767
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author De Magistris, Paola
author_facet De Magistris, Paola
author_sort De Magistris, Paola
collection PubMed
description Nuclear export of messenger RNA (mRNA) through the nuclear pore complex (NPC) is an indispensable step to ensure protein translation in the cytoplasm of eukaryotic cells. mRNA is not translocated on its own, but it forms ribonuclear particles (mRNPs) in association with proteins that are crucial for its metabolism, some of which; like Mex67/MTR2-NXF1/NXT1; are key players for its translocation to the cytoplasm. In this review, I will summarize our current body of knowledge on the basic characteristics of mRNA export through the NPC. To be granted passage, the mRNP cargo needs to bind transport receptors, which facilitate the nuclear export. During NPC transport, mRNPs undergo compositional and conformational changes. The interactions between mRNP and the central channel of NPC are described; together with the multiple quality control steps that mRNPs undergo at the different rings of the NPC to ensure only proper export of mature transcripts to the cytoplasm. I conclude by mentioning new opportunities that arise from bottom up approaches for a mechanistic understanding of nuclear export.
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spelling pubmed-85838452021-11-12 The Great Escape: mRNA Export through the Nuclear Pore Complex De Magistris, Paola Int J Mol Sci Review Nuclear export of messenger RNA (mRNA) through the nuclear pore complex (NPC) is an indispensable step to ensure protein translation in the cytoplasm of eukaryotic cells. mRNA is not translocated on its own, but it forms ribonuclear particles (mRNPs) in association with proteins that are crucial for its metabolism, some of which; like Mex67/MTR2-NXF1/NXT1; are key players for its translocation to the cytoplasm. In this review, I will summarize our current body of knowledge on the basic characteristics of mRNA export through the NPC. To be granted passage, the mRNP cargo needs to bind transport receptors, which facilitate the nuclear export. During NPC transport, mRNPs undergo compositional and conformational changes. The interactions between mRNP and the central channel of NPC are described; together with the multiple quality control steps that mRNPs undergo at the different rings of the NPC to ensure only proper export of mature transcripts to the cytoplasm. I conclude by mentioning new opportunities that arise from bottom up approaches for a mechanistic understanding of nuclear export. MDPI 2021-10-29 /pmc/articles/PMC8583845/ /pubmed/34769195 http://dx.doi.org/10.3390/ijms222111767 Text en © 2021 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
De Magistris, Paola
The Great Escape: mRNA Export through the Nuclear Pore Complex
title The Great Escape: mRNA Export through the Nuclear Pore Complex
title_full The Great Escape: mRNA Export through the Nuclear Pore Complex
title_fullStr The Great Escape: mRNA Export through the Nuclear Pore Complex
title_full_unstemmed The Great Escape: mRNA Export through the Nuclear Pore Complex
title_short The Great Escape: mRNA Export through the Nuclear Pore Complex
title_sort great escape: mrna export through the nuclear pore complex
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583845/
https://www.ncbi.nlm.nih.gov/pubmed/34769195
http://dx.doi.org/10.3390/ijms222111767
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