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Plasmid Curing and Exchange Using a Novel Counter-Selectable Marker Based on Unnatural Amino Acid Incorporation at a Sense Codon
A protocol was designed for plasmid curing using a novel counter-selectable marker, named pylS(ZK)-pylT, in Escherichia coli. The pylS(ZK)-pylT marker consists of the archaeal pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA (tRNA(pyl)) with modification, and incorporates an unnatural amino a...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MDPI
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583848/ https://www.ncbi.nlm.nih.gov/pubmed/34768910 http://dx.doi.org/10.3390/ijms222111482 |