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Ventricular TLR4 Levels Abrogate TLR2-Mediated Adverse Cardiac Remodeling upon Pressure Overload in Mice

Involvement of the Toll-like receptor 4 (TLR4) in maladaptive cardiac remodeling and heart failure (HF) upon pressure overload has been studied extensively, but less is known about the role of TLR2. Interplay and redundancy of TLR4 with TLR2 have been reported in other organs but were not investigat...

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Autores principales: Kessler, Elise L., Wang, Jiong-Wei, Kok, Bart, Brans, Maike A., Nederlof, Angelique, van Stuijvenberg, Leonie, Huang, Chenyuan, Vink, Aryan, Arslan, Fatih, Efimov, Igor R., Lam, Carolyn S. P., Vos, Marc A., de Kleijn, Dominique P. V., Fontes, Magda S. C., van Veen, Toon A. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583975/
https://www.ncbi.nlm.nih.gov/pubmed/34769252
http://dx.doi.org/10.3390/ijms222111823
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author Kessler, Elise L.
Wang, Jiong-Wei
Kok, Bart
Brans, Maike A.
Nederlof, Angelique
van Stuijvenberg, Leonie
Huang, Chenyuan
Vink, Aryan
Arslan, Fatih
Efimov, Igor R.
Lam, Carolyn S. P.
Vos, Marc A.
de Kleijn, Dominique P. V.
Fontes, Magda S. C.
van Veen, Toon A. B.
author_facet Kessler, Elise L.
Wang, Jiong-Wei
Kok, Bart
Brans, Maike A.
Nederlof, Angelique
van Stuijvenberg, Leonie
Huang, Chenyuan
Vink, Aryan
Arslan, Fatih
Efimov, Igor R.
Lam, Carolyn S. P.
Vos, Marc A.
de Kleijn, Dominique P. V.
Fontes, Magda S. C.
van Veen, Toon A. B.
author_sort Kessler, Elise L.
collection PubMed
description Involvement of the Toll-like receptor 4 (TLR4) in maladaptive cardiac remodeling and heart failure (HF) upon pressure overload has been studied extensively, but less is known about the role of TLR2. Interplay and redundancy of TLR4 with TLR2 have been reported in other organs but were not investigated during cardiac dysfunction. We explored whether TLR2 deficiency leads to less adverse cardiac remodeling upon chronic pressure overload and whether TLR2 and TLR4 additively contribute to this. We subjected 35 male C57BL/6J mice (wildtype (WT) or TLR2 knockout (KO)) to sham or transverse aortic constriction (TAC) surgery. After 12 weeks, echocardiography and electrocardiography were performed, and hearts were extracted for molecular and histological analysis. TLR2 deficiency (n = 14) was confirmed in all KO mice by PCR and resulted in less hypertrophy (heart weight to tibia length ratio (HW/TL), smaller cross-sectional cardiomyocyte area and decreased brain natriuretic peptide (BNP) mRNA expression, p < 0.05), increased contractility (QRS and QTc, p < 0.05), and less inflammation (e.g., interleukins 6 and 1β, p < 0.05) after TAC compared to WT animals (n = 11). Even though TLR2 KO TAC animals presented with lower levels of ventricular TLR4 mRNA than WT TAC animals (13.2 ± 0.8 vs. 16.6 ± 0.7 mg/mm, p < 0.01), TLR4 mRNA expression was increased in animals with the largest ventricular mass, highest hypertrophy, and lowest ejection fraction, leading to two distinct groups of TLR2 KO TAC animals with variations in cardiac remodeling. This variation, however, was not seen in WT TAC animals even though heart weight/tibia length correlated with expression of TLR4 in these animals (r = 0.078, p = 0.005). Our data suggest that TLR2 deficiency ameliorates adverse cardiac remodeling and that ventricular TLR2 and TLR4 additively contribute to adverse cardiac remodeling during chronic pressure overload. Therefore, both TLRs may be therapeutic targets to prevent or interfere in the underlying molecular processes.
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spelling pubmed-85839752021-11-12 Ventricular TLR4 Levels Abrogate TLR2-Mediated Adverse Cardiac Remodeling upon Pressure Overload in Mice Kessler, Elise L. Wang, Jiong-Wei Kok, Bart Brans, Maike A. Nederlof, Angelique van Stuijvenberg, Leonie Huang, Chenyuan Vink, Aryan Arslan, Fatih Efimov, Igor R. Lam, Carolyn S. P. Vos, Marc A. de Kleijn, Dominique P. V. Fontes, Magda S. C. van Veen, Toon A. B. Int J Mol Sci Article Involvement of the Toll-like receptor 4 (TLR4) in maladaptive cardiac remodeling and heart failure (HF) upon pressure overload has been studied extensively, but less is known about the role of TLR2. Interplay and redundancy of TLR4 with TLR2 have been reported in other organs but were not investigated during cardiac dysfunction. We explored whether TLR2 deficiency leads to less adverse cardiac remodeling upon chronic pressure overload and whether TLR2 and TLR4 additively contribute to this. We subjected 35 male C57BL/6J mice (wildtype (WT) or TLR2 knockout (KO)) to sham or transverse aortic constriction (TAC) surgery. After 12 weeks, echocardiography and electrocardiography were performed, and hearts were extracted for molecular and histological analysis. TLR2 deficiency (n = 14) was confirmed in all KO mice by PCR and resulted in less hypertrophy (heart weight to tibia length ratio (HW/TL), smaller cross-sectional cardiomyocyte area and decreased brain natriuretic peptide (BNP) mRNA expression, p < 0.05), increased contractility (QRS and QTc, p < 0.05), and less inflammation (e.g., interleukins 6 and 1β, p < 0.05) after TAC compared to WT animals (n = 11). Even though TLR2 KO TAC animals presented with lower levels of ventricular TLR4 mRNA than WT TAC animals (13.2 ± 0.8 vs. 16.6 ± 0.7 mg/mm, p < 0.01), TLR4 mRNA expression was increased in animals with the largest ventricular mass, highest hypertrophy, and lowest ejection fraction, leading to two distinct groups of TLR2 KO TAC animals with variations in cardiac remodeling. This variation, however, was not seen in WT TAC animals even though heart weight/tibia length correlated with expression of TLR4 in these animals (r = 0.078, p = 0.005). Our data suggest that TLR2 deficiency ameliorates adverse cardiac remodeling and that ventricular TLR2 and TLR4 additively contribute to adverse cardiac remodeling during chronic pressure overload. Therefore, both TLRs may be therapeutic targets to prevent or interfere in the underlying molecular processes. MDPI 2021-10-30 /pmc/articles/PMC8583975/ /pubmed/34769252 http://dx.doi.org/10.3390/ijms222111823 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kessler, Elise L.
Wang, Jiong-Wei
Kok, Bart
Brans, Maike A.
Nederlof, Angelique
van Stuijvenberg, Leonie
Huang, Chenyuan
Vink, Aryan
Arslan, Fatih
Efimov, Igor R.
Lam, Carolyn S. P.
Vos, Marc A.
de Kleijn, Dominique P. V.
Fontes, Magda S. C.
van Veen, Toon A. B.
Ventricular TLR4 Levels Abrogate TLR2-Mediated Adverse Cardiac Remodeling upon Pressure Overload in Mice
title Ventricular TLR4 Levels Abrogate TLR2-Mediated Adverse Cardiac Remodeling upon Pressure Overload in Mice
title_full Ventricular TLR4 Levels Abrogate TLR2-Mediated Adverse Cardiac Remodeling upon Pressure Overload in Mice
title_fullStr Ventricular TLR4 Levels Abrogate TLR2-Mediated Adverse Cardiac Remodeling upon Pressure Overload in Mice
title_full_unstemmed Ventricular TLR4 Levels Abrogate TLR2-Mediated Adverse Cardiac Remodeling upon Pressure Overload in Mice
title_short Ventricular TLR4 Levels Abrogate TLR2-Mediated Adverse Cardiac Remodeling upon Pressure Overload in Mice
title_sort ventricular tlr4 levels abrogate tlr2-mediated adverse cardiac remodeling upon pressure overload in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8583975/
https://www.ncbi.nlm.nih.gov/pubmed/34769252
http://dx.doi.org/10.3390/ijms222111823
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