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Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

While prime editing enables precise sequence changes in DNA, cellular determinants of prime editing remain poorly understood. Using pooled CRISPRi screens, we discovered that DNA mismatch repair (MMR) impedes prime editing and promotes undesired indel byproducts. We developed PE4 and PE5 prime editi...

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Detalles Bibliográficos
Autores principales:	Chen, Peter J., Hussmann, Jeffrey A., Yan, Jun, Knipping, Friederike, Ravisankar, Purnima, Chen, Pin-Fang, Chen, Cidi, Nelson, James W., Newby, Gregory A., Sahin, Mustafa, Osborn, Mark J., Weissman, Jonathan S., Adamson, Britt, Liu, David R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cell Press 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584034/ https://www.ncbi.nlm.nih.gov/pubmed/34653350 http://dx.doi.org/10.1016/j.cell.2021.09.018

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