Cargando…
An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes
Prostate cancer (PCa) in dogs is a highly malignant disease akin to its human counterpart. In contrast to the situation in humans, multi-gene approaches facilitating risk stratification of canine PCa are barely established. The aims of this study were the characterization of the transcriptional land...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584104/ https://www.ncbi.nlm.nih.gov/pubmed/34768937 http://dx.doi.org/10.3390/ijms222111481 |
_version_ | 1784597366049865728 |
---|---|
author | Thiemeyer, Heike Taher, Leila Schille, Jan Torben Packeiser, Eva-Maria Harder, Lisa K. Hewicker-Trautwein, Marion Brenig, Bertram Schütz, Ekkehard Beck, Julia Nolte, Ingo Murua Escobar, Hugo |
author_facet | Thiemeyer, Heike Taher, Leila Schille, Jan Torben Packeiser, Eva-Maria Harder, Lisa K. Hewicker-Trautwein, Marion Brenig, Bertram Schütz, Ekkehard Beck, Julia Nolte, Ingo Murua Escobar, Hugo |
author_sort | Thiemeyer, Heike |
collection | PubMed |
description | Prostate cancer (PCa) in dogs is a highly malignant disease akin to its human counterpart. In contrast to the situation in humans, multi-gene approaches facilitating risk stratification of canine PCa are barely established. The aims of this study were the characterization of the transcriptional landscape of canine PCa and the identification of diagnostic, prognostic and/or therapeutic biomarkers through a multi-step screening approach. RNA-Sequencing of ten malignant tissues and fine-needle aspirations (FNA), and 14 nonmalignant tissues and FNAs was performed to find differentially expressed genes (DEGs) and deregulated pathways. The 4098 observed DEGs were involved in 49 pathways. These 49 pathways could be grouped into five superpathways summarizing the hallmarks of canine PCa: (i) inflammatory response and cytokines; (ii) regulation of the immune system and cell death; (iii) cell surface and PI3K signaling; (iv) cell cycle; and (v) phagosome and autophagy. Among the highly deregulated, moderately to strongly expressed DEGs that were members of one or more superpathways, 169 DEGs were listed in relevant databases and/or the literature and included members of the PCa pathway, oncogenes, prostate-specific genes, and druggable genes. These genes are novel and promising candidate diagnostic, prognostic and/or therapeutic canine PCa biomarkers. |
format | Online Article Text |
id | pubmed-8584104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85841042021-11-12 An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes Thiemeyer, Heike Taher, Leila Schille, Jan Torben Packeiser, Eva-Maria Harder, Lisa K. Hewicker-Trautwein, Marion Brenig, Bertram Schütz, Ekkehard Beck, Julia Nolte, Ingo Murua Escobar, Hugo Int J Mol Sci Article Prostate cancer (PCa) in dogs is a highly malignant disease akin to its human counterpart. In contrast to the situation in humans, multi-gene approaches facilitating risk stratification of canine PCa are barely established. The aims of this study were the characterization of the transcriptional landscape of canine PCa and the identification of diagnostic, prognostic and/or therapeutic biomarkers through a multi-step screening approach. RNA-Sequencing of ten malignant tissues and fine-needle aspirations (FNA), and 14 nonmalignant tissues and FNAs was performed to find differentially expressed genes (DEGs) and deregulated pathways. The 4098 observed DEGs were involved in 49 pathways. These 49 pathways could be grouped into five superpathways summarizing the hallmarks of canine PCa: (i) inflammatory response and cytokines; (ii) regulation of the immune system and cell death; (iii) cell surface and PI3K signaling; (iv) cell cycle; and (v) phagosome and autophagy. Among the highly deregulated, moderately to strongly expressed DEGs that were members of one or more superpathways, 169 DEGs were listed in relevant databases and/or the literature and included members of the PCa pathway, oncogenes, prostate-specific genes, and druggable genes. These genes are novel and promising candidate diagnostic, prognostic and/or therapeutic canine PCa biomarkers. MDPI 2021-10-25 /pmc/articles/PMC8584104/ /pubmed/34768937 http://dx.doi.org/10.3390/ijms222111481 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Thiemeyer, Heike Taher, Leila Schille, Jan Torben Packeiser, Eva-Maria Harder, Lisa K. Hewicker-Trautwein, Marion Brenig, Bertram Schütz, Ekkehard Beck, Julia Nolte, Ingo Murua Escobar, Hugo An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes |
title | An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes |
title_full | An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes |
title_fullStr | An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes |
title_full_unstemmed | An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes |
title_short | An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes |
title_sort | rna-seq-based framework for characterizing canine prostate cancer and prioritizing clinically relevant biomarker candidate genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584104/ https://www.ncbi.nlm.nih.gov/pubmed/34768937 http://dx.doi.org/10.3390/ijms222111481 |
work_keys_str_mv | AT thiemeyerheike anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT taherleila anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT schillejantorben anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT packeiserevamaria anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT harderlisak anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT hewickertrautweinmarion anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT brenigbertram anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT schutzekkehard anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT beckjulia anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT nolteingo anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT muruaescobarhugo anrnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT thiemeyerheike rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT taherleila rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT schillejantorben rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT packeiserevamaria rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT harderlisak rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT hewickertrautweinmarion rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT brenigbertram rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT schutzekkehard rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT beckjulia rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT nolteingo rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes AT muruaescobarhugo rnaseqbasedframeworkforcharacterizingcanineprostatecancerandprioritizingclinicallyrelevantbiomarkercandidategenes |