Cargando…

Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy

Impaired motor and sensory functions are the main features of Charcot–Marie–Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with h...

Descripción completa

Detalles Bibliográficos
Autores principales: Govbakh, Iryna, Kyryk, Vitalii, Ustymenko, Alina, Rubtsov, Volodymyr, Tsupykov, Oleg, Bulgakova, Nataliya V., Zavodovskiy, Danylo O., Sokolowska, Inna, Maznychenko, Andriy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584487/
https://www.ncbi.nlm.nih.gov/pubmed/34769453
http://dx.doi.org/10.3390/ijms222112026
_version_ 1784597461104328704
author Govbakh, Iryna
Kyryk, Vitalii
Ustymenko, Alina
Rubtsov, Volodymyr
Tsupykov, Oleg
Bulgakova, Nataliya V.
Zavodovskiy, Danylo O.
Sokolowska, Inna
Maznychenko, Andriy
author_facet Govbakh, Iryna
Kyryk, Vitalii
Ustymenko, Alina
Rubtsov, Volodymyr
Tsupykov, Oleg
Bulgakova, Nataliya V.
Zavodovskiy, Danylo O.
Sokolowska, Inna
Maznychenko, Andriy
author_sort Govbakh, Iryna
collection PubMed
description Impaired motor and sensory functions are the main features of Charcot–Marie–Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with hereditary peripheral neuropathy. Murine adipose-derived mesenchymal stromal cells (AD-MSCs) were obtained for transplantation into TS muscles of FVB-C-Tg(GFPU)5Nagy/J mice. Three months after AD-MSCs transplantation, animals were subjected to electrophysiological investigations. Parameters of TS muscle tension after intermittent high frequency electrical sciatic nerve stimulations were analyzed. It was found that force of TS muscle tension contraction in animals after AD-MSCs treatment was two-time higher than in untreated mice. Normalized values of force muscle contraction in different phases of electrical stimulation were 0.3 ± 0.01 vs. 0.18 ± 0.01 and 0.26 ± 0.03 vs. 0.13 ± 0.03 for treated and untreated animals, respectively. It is assumed that the two-fold increase in TS muscle strength was caused by stem cell therapy. Apparently, AD-MSCs therapy can promote nerve regeneration and partial restoration of muscle function, and thus can be a potential therapeutic agent for the treatment of peripheral neuropathies.
format Online
Article
Text
id pubmed-8584487
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85844872021-11-12 Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy Govbakh, Iryna Kyryk, Vitalii Ustymenko, Alina Rubtsov, Volodymyr Tsupykov, Oleg Bulgakova, Nataliya V. Zavodovskiy, Danylo O. Sokolowska, Inna Maznychenko, Andriy Int J Mol Sci Article Impaired motor and sensory functions are the main features of Charcot–Marie–Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with hereditary peripheral neuropathy. Murine adipose-derived mesenchymal stromal cells (AD-MSCs) were obtained for transplantation into TS muscles of FVB-C-Tg(GFPU)5Nagy/J mice. Three months after AD-MSCs transplantation, animals were subjected to electrophysiological investigations. Parameters of TS muscle tension after intermittent high frequency electrical sciatic nerve stimulations were analyzed. It was found that force of TS muscle tension contraction in animals after AD-MSCs treatment was two-time higher than in untreated mice. Normalized values of force muscle contraction in different phases of electrical stimulation were 0.3 ± 0.01 vs. 0.18 ± 0.01 and 0.26 ± 0.03 vs. 0.13 ± 0.03 for treated and untreated animals, respectively. It is assumed that the two-fold increase in TS muscle strength was caused by stem cell therapy. Apparently, AD-MSCs therapy can promote nerve regeneration and partial restoration of muscle function, and thus can be a potential therapeutic agent for the treatment of peripheral neuropathies. MDPI 2021-11-06 /pmc/articles/PMC8584487/ /pubmed/34769453 http://dx.doi.org/10.3390/ijms222112026 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Govbakh, Iryna
Kyryk, Vitalii
Ustymenko, Alina
Rubtsov, Volodymyr
Tsupykov, Oleg
Bulgakova, Nataliya V.
Zavodovskiy, Danylo O.
Sokolowska, Inna
Maznychenko, Andriy
Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_full Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_fullStr Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_full_unstemmed Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_short Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
title_sort stem cell therapy enhances motor activity of triceps surae muscle in mice with hereditary peripheral neuropathy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584487/
https://www.ncbi.nlm.nih.gov/pubmed/34769453
http://dx.doi.org/10.3390/ijms222112026
work_keys_str_mv AT govbakhiryna stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy
AT kyrykvitalii stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy
AT ustymenkoalina stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy
AT rubtsovvolodymyr stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy
AT tsupykovoleg stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy
AT bulgakovanataliyav stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy
AT zavodovskiydanyloo stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy
AT sokolowskainna stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy
AT maznychenkoandriy stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy