Cargando…
Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy
Impaired motor and sensory functions are the main features of Charcot–Marie–Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with h...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584487/ https://www.ncbi.nlm.nih.gov/pubmed/34769453 http://dx.doi.org/10.3390/ijms222112026 |
_version_ | 1784597461104328704 |
---|---|
author | Govbakh, Iryna Kyryk, Vitalii Ustymenko, Alina Rubtsov, Volodymyr Tsupykov, Oleg Bulgakova, Nataliya V. Zavodovskiy, Danylo O. Sokolowska, Inna Maznychenko, Andriy |
author_facet | Govbakh, Iryna Kyryk, Vitalii Ustymenko, Alina Rubtsov, Volodymyr Tsupykov, Oleg Bulgakova, Nataliya V. Zavodovskiy, Danylo O. Sokolowska, Inna Maznychenko, Andriy |
author_sort | Govbakh, Iryna |
collection | PubMed |
description | Impaired motor and sensory functions are the main features of Charcot–Marie–Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with hereditary peripheral neuropathy. Murine adipose-derived mesenchymal stromal cells (AD-MSCs) were obtained for transplantation into TS muscles of FVB-C-Tg(GFPU)5Nagy/J mice. Three months after AD-MSCs transplantation, animals were subjected to electrophysiological investigations. Parameters of TS muscle tension after intermittent high frequency electrical sciatic nerve stimulations were analyzed. It was found that force of TS muscle tension contraction in animals after AD-MSCs treatment was two-time higher than in untreated mice. Normalized values of force muscle contraction in different phases of electrical stimulation were 0.3 ± 0.01 vs. 0.18 ± 0.01 and 0.26 ± 0.03 vs. 0.13 ± 0.03 for treated and untreated animals, respectively. It is assumed that the two-fold increase in TS muscle strength was caused by stem cell therapy. Apparently, AD-MSCs therapy can promote nerve regeneration and partial restoration of muscle function, and thus can be a potential therapeutic agent for the treatment of peripheral neuropathies. |
format | Online Article Text |
id | pubmed-8584487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85844872021-11-12 Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy Govbakh, Iryna Kyryk, Vitalii Ustymenko, Alina Rubtsov, Volodymyr Tsupykov, Oleg Bulgakova, Nataliya V. Zavodovskiy, Danylo O. Sokolowska, Inna Maznychenko, Andriy Int J Mol Sci Article Impaired motor and sensory functions are the main features of Charcot–Marie–Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with hereditary peripheral neuropathy. Murine adipose-derived mesenchymal stromal cells (AD-MSCs) were obtained for transplantation into TS muscles of FVB-C-Tg(GFPU)5Nagy/J mice. Three months after AD-MSCs transplantation, animals were subjected to electrophysiological investigations. Parameters of TS muscle tension after intermittent high frequency electrical sciatic nerve stimulations were analyzed. It was found that force of TS muscle tension contraction in animals after AD-MSCs treatment was two-time higher than in untreated mice. Normalized values of force muscle contraction in different phases of electrical stimulation were 0.3 ± 0.01 vs. 0.18 ± 0.01 and 0.26 ± 0.03 vs. 0.13 ± 0.03 for treated and untreated animals, respectively. It is assumed that the two-fold increase in TS muscle strength was caused by stem cell therapy. Apparently, AD-MSCs therapy can promote nerve regeneration and partial restoration of muscle function, and thus can be a potential therapeutic agent for the treatment of peripheral neuropathies. MDPI 2021-11-06 /pmc/articles/PMC8584487/ /pubmed/34769453 http://dx.doi.org/10.3390/ijms222112026 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Govbakh, Iryna Kyryk, Vitalii Ustymenko, Alina Rubtsov, Volodymyr Tsupykov, Oleg Bulgakova, Nataliya V. Zavodovskiy, Danylo O. Sokolowska, Inna Maznychenko, Andriy Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy |
title | Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy |
title_full | Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy |
title_fullStr | Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy |
title_full_unstemmed | Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy |
title_short | Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy |
title_sort | stem cell therapy enhances motor activity of triceps surae muscle in mice with hereditary peripheral neuropathy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584487/ https://www.ncbi.nlm.nih.gov/pubmed/34769453 http://dx.doi.org/10.3390/ijms222112026 |
work_keys_str_mv | AT govbakhiryna stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy AT kyrykvitalii stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy AT ustymenkoalina stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy AT rubtsovvolodymyr stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy AT tsupykovoleg stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy AT bulgakovanataliyav stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy AT zavodovskiydanyloo stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy AT sokolowskainna stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy AT maznychenkoandriy stemcelltherapyenhancesmotoractivityoftricepssuraemuscleinmicewithhereditaryperipheralneuropathy |