Cargando…
Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals
This study evaluated the presence of Human Papillomavirus (HPV) DNA in the cervix and peripheral blood of women with cervical intraepithelial neoplasia (CIN I, II, and III) and healthy individuals. Overall, 139 paired peripheral blood and cervix samples of healthy women and women with CIN I, II, and...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584517/ https://www.ncbi.nlm.nih.gov/pubmed/34768729 http://dx.doi.org/10.3390/jcm10215209 |
_version_ | 1784597468505178112 |
---|---|
author | Conceição Gomes Nascimento, Kamylla Gonçalves Lima, Élyda Mota Nunes, Zhilbelly Rêgo Barros Júnior, Marconi de Aragão Batista, Marcus Vinícius Lucena Araujo, Antonio Roberto da Costa Silva Neto, Jacinto Simas Chagas, Bárbara Almeida Diniz Gurgel, Ana Pavla de Freitas, Antonio Carlos |
author_facet | Conceição Gomes Nascimento, Kamylla Gonçalves Lima, Élyda Mota Nunes, Zhilbelly Rêgo Barros Júnior, Marconi de Aragão Batista, Marcus Vinícius Lucena Araujo, Antonio Roberto da Costa Silva Neto, Jacinto Simas Chagas, Bárbara Almeida Diniz Gurgel, Ana Pavla de Freitas, Antonio Carlos |
author_sort | Conceição Gomes Nascimento, Kamylla |
collection | PubMed |
description | This study evaluated the presence of Human Papillomavirus (HPV) DNA in the cervix and peripheral blood of women with cervical intraepithelial neoplasia (CIN I, II, and III) and healthy individuals. Overall, 139 paired peripheral blood and cervix samples of healthy women and women with CIN I, II, and III (n = 68) were tested for HPV DNA by using standard procedures. Polymerase chain reaction (PCR) sequencing determined HPV types. Quantification of HPV16 E6 and E2 genes was performed to determine viral load and physical state. HPV DNA was detected in the cervix (21.1% in healthy individuals; 48.8–55.5% in CIN patients), blood (46.4% in healthy individuals; 44.1–77.7% in CIN patients) and paired peripheral blood and cervix samples (24% in healthy individuals; 32.5–44.4% in CIN patients). The most frequent types found in the cervix were HPV16, 18, 31, 33, 58, and 70, while HPV16, 18, 33, 58, and 66 were the most frequent types found in the blood. HPV DNA in the cervix was associated with previous sexually transmitted infections (STIs) (p = 0.023; OR: 2.978; CI:1.34–7.821), HPV DNA in the blood (p = 0.000; OR: 8.283; CI:3.700–18.540), and cervical lesions (CIN I/II or III) (p = 0.007). Binomial logistic regression showed that HPV DNA in the blood (p = 0.000; OR: 9.324; CI:3.612–24.072) and cervical lesions (p = 0.011; OR: 3.622; CI:1.338–9.806) were associated with HPV DNA in the cervix. However, we did not find an association between HPV DNA in the blood and cervical lesions (p = 0.385). Our results showed that only HPV DNA found in the cervix was associated with cervical lesions. |
format | Online Article Text |
id | pubmed-8584517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85845172021-11-12 Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals Conceição Gomes Nascimento, Kamylla Gonçalves Lima, Élyda Mota Nunes, Zhilbelly Rêgo Barros Júnior, Marconi de Aragão Batista, Marcus Vinícius Lucena Araujo, Antonio Roberto da Costa Silva Neto, Jacinto Simas Chagas, Bárbara Almeida Diniz Gurgel, Ana Pavla de Freitas, Antonio Carlos J Clin Med Article This study evaluated the presence of Human Papillomavirus (HPV) DNA in the cervix and peripheral blood of women with cervical intraepithelial neoplasia (CIN I, II, and III) and healthy individuals. Overall, 139 paired peripheral blood and cervix samples of healthy women and women with CIN I, II, and III (n = 68) were tested for HPV DNA by using standard procedures. Polymerase chain reaction (PCR) sequencing determined HPV types. Quantification of HPV16 E6 and E2 genes was performed to determine viral load and physical state. HPV DNA was detected in the cervix (21.1% in healthy individuals; 48.8–55.5% in CIN patients), blood (46.4% in healthy individuals; 44.1–77.7% in CIN patients) and paired peripheral blood and cervix samples (24% in healthy individuals; 32.5–44.4% in CIN patients). The most frequent types found in the cervix were HPV16, 18, 31, 33, 58, and 70, while HPV16, 18, 33, 58, and 66 were the most frequent types found in the blood. HPV DNA in the cervix was associated with previous sexually transmitted infections (STIs) (p = 0.023; OR: 2.978; CI:1.34–7.821), HPV DNA in the blood (p = 0.000; OR: 8.283; CI:3.700–18.540), and cervical lesions (CIN I/II or III) (p = 0.007). Binomial logistic regression showed that HPV DNA in the blood (p = 0.000; OR: 9.324; CI:3.612–24.072) and cervical lesions (p = 0.011; OR: 3.622; CI:1.338–9.806) were associated with HPV DNA in the cervix. However, we did not find an association between HPV DNA in the blood and cervical lesions (p = 0.385). Our results showed that only HPV DNA found in the cervix was associated with cervical lesions. MDPI 2021-11-08 /pmc/articles/PMC8584517/ /pubmed/34768729 http://dx.doi.org/10.3390/jcm10215209 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Conceição Gomes Nascimento, Kamylla Gonçalves Lima, Élyda Mota Nunes, Zhilbelly Rêgo Barros Júnior, Marconi de Aragão Batista, Marcus Vinícius Lucena Araujo, Antonio Roberto da Costa Silva Neto, Jacinto Simas Chagas, Bárbara Almeida Diniz Gurgel, Ana Pavla de Freitas, Antonio Carlos Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals |
title | Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals |
title_full | Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals |
title_fullStr | Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals |
title_full_unstemmed | Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals |
title_short | Detection of Human Papillomavirus DNA in Paired Peripheral Blood and Cervix Samples in Patients with Cervical Lesions and Healthy Individuals |
title_sort | detection of human papillomavirus dna in paired peripheral blood and cervix samples in patients with cervical lesions and healthy individuals |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584517/ https://www.ncbi.nlm.nih.gov/pubmed/34768729 http://dx.doi.org/10.3390/jcm10215209 |
work_keys_str_mv | AT conceicaogomesnascimentokamylla detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals AT goncalveslimaelyda detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals AT motanuneszhilbelly detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals AT regobarrosjuniormarconi detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals AT dearagaobatistamarcusvinicius detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals AT lucenaaraujoantonioroberto detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals AT dacostasilvanetojacinto detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals AT simaschagasbarbara detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals AT almeidadinizgurgelanapavla detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals AT defreitasantoniocarlos detectionofhumanpapillomavirusdnainpairedperipheralbloodandcervixsamplesinpatientswithcervicallesionsandhealthyindividuals |