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The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells
Mutations in the PINK1 and PRKN genes are the most common cause of early-onset familial Parkinson disease. These genes code for the PINK1 and Parkin proteins, respectively, which are involved in the degradation of dysfunctional mitochondria through mitophagy. An early step in PINK1 –Parkin mediated...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584748/ https://www.ncbi.nlm.nih.gov/pubmed/34762687 http://dx.doi.org/10.1371/journal.pone.0259903 |
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author | Bradshaw, Aaron V. Campbell, Philip Schapira, Anthony H. V. Morris, Huw R. Taanman, Jan-Willem |
author_facet | Bradshaw, Aaron V. Campbell, Philip Schapira, Anthony H. V. Morris, Huw R. Taanman, Jan-Willem |
author_sort | Bradshaw, Aaron V. |
collection | PubMed |
description | Mutations in the PINK1 and PRKN genes are the most common cause of early-onset familial Parkinson disease. These genes code for the PINK1 and Parkin proteins, respectively, which are involved in the degradation of dysfunctional mitochondria through mitophagy. An early step in PINK1 –Parkin mediated mitophagy is the ubiquitination of the mitofusin proteins MFN1 and -2. The ubiquitination of MFN1 and -2 in patient samples may therefore serve as a biomarker to determine the functional effects of PINK1 and PRKN mutations, and to screen idiopathic patients for potential mitophagy defects. We aimed to characterise the expression of the PINK1 –Parkin mitophagy machinery in peripheral blood mononuclear cells (PBMCs) and assess if these cells could serve as a platform to evaluate mitophagy via analysis of MFN1 and -2 ubiquitination. Mitophagy was induced through mitochondrial depolarisation by treatment with the protonophore CCCP and ubiquitinated MFN proteins were analysed by western blotting. In addition, PINK1 and PRKN mRNA and protein expression levels were characterised with reverse transcriptase quantitative PCR and western blotting, respectively. Whilst CCCP treatment led to MFN ubiquitination in primary fibroblasts, SH-SY5Y neuroblastoma cells and Jurkat leukaemic cells, treatment of PBMCs did not induce ubiquitination of MFN. PRKN mRNA and protein was readily detectable in PBMCs at comparable levels to those observed in Jurkat and fibroblast cells. In contrast, PINK1 protein was undetectable and PINK1 mRNA levels were remarkably low in control PBMCs. Our findings suggest that the PINK1 –Parkin mitophagy signalling pathway is not functional in PBMCs. Therefore, PBMCs are not a suitable biosample for analysis of mitophagy function in Parkinson disease patients. |
format | Online Article Text |
id | pubmed-8584748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-85847482021-11-12 The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells Bradshaw, Aaron V. Campbell, Philip Schapira, Anthony H. V. Morris, Huw R. Taanman, Jan-Willem PLoS One Research Article Mutations in the PINK1 and PRKN genes are the most common cause of early-onset familial Parkinson disease. These genes code for the PINK1 and Parkin proteins, respectively, which are involved in the degradation of dysfunctional mitochondria through mitophagy. An early step in PINK1 –Parkin mediated mitophagy is the ubiquitination of the mitofusin proteins MFN1 and -2. The ubiquitination of MFN1 and -2 in patient samples may therefore serve as a biomarker to determine the functional effects of PINK1 and PRKN mutations, and to screen idiopathic patients for potential mitophagy defects. We aimed to characterise the expression of the PINK1 –Parkin mitophagy machinery in peripheral blood mononuclear cells (PBMCs) and assess if these cells could serve as a platform to evaluate mitophagy via analysis of MFN1 and -2 ubiquitination. Mitophagy was induced through mitochondrial depolarisation by treatment with the protonophore CCCP and ubiquitinated MFN proteins were analysed by western blotting. In addition, PINK1 and PRKN mRNA and protein expression levels were characterised with reverse transcriptase quantitative PCR and western blotting, respectively. Whilst CCCP treatment led to MFN ubiquitination in primary fibroblasts, SH-SY5Y neuroblastoma cells and Jurkat leukaemic cells, treatment of PBMCs did not induce ubiquitination of MFN. PRKN mRNA and protein was readily detectable in PBMCs at comparable levels to those observed in Jurkat and fibroblast cells. In contrast, PINK1 protein was undetectable and PINK1 mRNA levels were remarkably low in control PBMCs. Our findings suggest that the PINK1 –Parkin mitophagy signalling pathway is not functional in PBMCs. Therefore, PBMCs are not a suitable biosample for analysis of mitophagy function in Parkinson disease patients. Public Library of Science 2021-11-11 /pmc/articles/PMC8584748/ /pubmed/34762687 http://dx.doi.org/10.1371/journal.pone.0259903 Text en © 2021 Bradshaw et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bradshaw, Aaron V. Campbell, Philip Schapira, Anthony H. V. Morris, Huw R. Taanman, Jan-Willem The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells |
title | The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells |
title_full | The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells |
title_fullStr | The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells |
title_full_unstemmed | The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells |
title_short | The PINK1—Parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells |
title_sort | pink1—parkin mitophagy signalling pathway is not functional in peripheral blood mononuclear cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584748/ https://www.ncbi.nlm.nih.gov/pubmed/34762687 http://dx.doi.org/10.1371/journal.pone.0259903 |
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