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CaWRKY30 Positively Regulates Pepper Immunity by Targeting CaWRKY40 against Ralstonia solanacearum Inoculation through Modulating Defense-Related Genes

The WRKY transcription factors (TFs) network is composed of WRKY TFs’ subset, which performs a critical role in immunity regulation of plants. However, functions of WRKY TFs’ network remain unclear, particularly in non-model plants such as pepper (Capsicum annuum L.). This study functionally charact...

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Autores principales: Hussain, Ansar, Khan, Muhammad Ifnan, Albaqami, Mohammed, Mahpara, Shahzadi, Noorka, Ijaz Rasool, Ahmed, Mohamed A. A., Aljuaid, Bandar S., El-Shehawi, Ahmed M., Liu, Zhiqin, Farooq, Shahid, Zuan, Ali Tan Kee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584995/
https://www.ncbi.nlm.nih.gov/pubmed/34769521
http://dx.doi.org/10.3390/ijms222112091
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author Hussain, Ansar
Khan, Muhammad Ifnan
Albaqami, Mohammed
Mahpara, Shahzadi
Noorka, Ijaz Rasool
Ahmed, Mohamed A. A.
Aljuaid, Bandar S.
El-Shehawi, Ahmed M.
Liu, Zhiqin
Farooq, Shahid
Zuan, Ali Tan Kee
author_facet Hussain, Ansar
Khan, Muhammad Ifnan
Albaqami, Mohammed
Mahpara, Shahzadi
Noorka, Ijaz Rasool
Ahmed, Mohamed A. A.
Aljuaid, Bandar S.
El-Shehawi, Ahmed M.
Liu, Zhiqin
Farooq, Shahid
Zuan, Ali Tan Kee
author_sort Hussain, Ansar
collection PubMed
description The WRKY transcription factors (TFs) network is composed of WRKY TFs’ subset, which performs a critical role in immunity regulation of plants. However, functions of WRKY TFs’ network remain unclear, particularly in non-model plants such as pepper (Capsicum annuum L.). This study functionally characterized CaWRKY30—a member of group III Pepper WRKY protein—for immunity of pepper against Ralstonia solanacearum infection. The CaWRKY30 was detected in nucleus, and its transcriptional expression levels were significantly upregulated by R. solanacearum inoculation (RSI), and foliar application ethylene (ET), abscisic acid (ABA), and salicylic acid (SA). Virus induced gene silencing (VIGS) of CaWRKY30 amplified pepper’s vulnerability to RSI. Additionally, the silencing of CaWRKY30 by VIGS compromised HR-like cell death triggered by RSI and downregulated defense-associated marker genes, like CaPR1, CaNPR1, CaDEF1, CaABR1, CaHIR1, and CaWRKY40. Conversely, transient over-expression of CaWRKY30 in pepper leaves instigated HR-like cell death and upregulated defense-related maker genes. Furthermore, transient over-expression of CaWRKY30 upregulated transcriptional levels of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40. On the other hand, transient over-expression of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40 upregulated transcriptional expression levels of CaWRKY30. The results recommend that newly characterized CaWRKY30 positively regulates pepper’s immunity against Ralstonia attack, which is governed by synergistically mediated signaling by phytohormones like ET, ABA, and SA, and transcriptionally assimilating into WRKY TFs networks, consisting of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40. Collectively, our data will facilitate to explicate the underlying mechanism of crosstalk between pepper’s immunity and response to RSI.
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spelling pubmed-85849952021-11-12 CaWRKY30 Positively Regulates Pepper Immunity by Targeting CaWRKY40 against Ralstonia solanacearum Inoculation through Modulating Defense-Related Genes Hussain, Ansar Khan, Muhammad Ifnan Albaqami, Mohammed Mahpara, Shahzadi Noorka, Ijaz Rasool Ahmed, Mohamed A. A. Aljuaid, Bandar S. El-Shehawi, Ahmed M. Liu, Zhiqin Farooq, Shahid Zuan, Ali Tan Kee Int J Mol Sci Article The WRKY transcription factors (TFs) network is composed of WRKY TFs’ subset, which performs a critical role in immunity regulation of plants. However, functions of WRKY TFs’ network remain unclear, particularly in non-model plants such as pepper (Capsicum annuum L.). This study functionally characterized CaWRKY30—a member of group III Pepper WRKY protein—for immunity of pepper against Ralstonia solanacearum infection. The CaWRKY30 was detected in nucleus, and its transcriptional expression levels were significantly upregulated by R. solanacearum inoculation (RSI), and foliar application ethylene (ET), abscisic acid (ABA), and salicylic acid (SA). Virus induced gene silencing (VIGS) of CaWRKY30 amplified pepper’s vulnerability to RSI. Additionally, the silencing of CaWRKY30 by VIGS compromised HR-like cell death triggered by RSI and downregulated defense-associated marker genes, like CaPR1, CaNPR1, CaDEF1, CaABR1, CaHIR1, and CaWRKY40. Conversely, transient over-expression of CaWRKY30 in pepper leaves instigated HR-like cell death and upregulated defense-related maker genes. Furthermore, transient over-expression of CaWRKY30 upregulated transcriptional levels of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40. On the other hand, transient over-expression of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40 upregulated transcriptional expression levels of CaWRKY30. The results recommend that newly characterized CaWRKY30 positively regulates pepper’s immunity against Ralstonia attack, which is governed by synergistically mediated signaling by phytohormones like ET, ABA, and SA, and transcriptionally assimilating into WRKY TFs networks, consisting of CaWRKY6, CaWRKY22, CaWRKY27, and CaWRKY40. Collectively, our data will facilitate to explicate the underlying mechanism of crosstalk between pepper’s immunity and response to RSI. MDPI 2021-11-08 /pmc/articles/PMC8584995/ /pubmed/34769521 http://dx.doi.org/10.3390/ijms222112091 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hussain, Ansar
Khan, Muhammad Ifnan
Albaqami, Mohammed
Mahpara, Shahzadi
Noorka, Ijaz Rasool
Ahmed, Mohamed A. A.
Aljuaid, Bandar S.
El-Shehawi, Ahmed M.
Liu, Zhiqin
Farooq, Shahid
Zuan, Ali Tan Kee
CaWRKY30 Positively Regulates Pepper Immunity by Targeting CaWRKY40 against Ralstonia solanacearum Inoculation through Modulating Defense-Related Genes
title CaWRKY30 Positively Regulates Pepper Immunity by Targeting CaWRKY40 against Ralstonia solanacearum Inoculation through Modulating Defense-Related Genes
title_full CaWRKY30 Positively Regulates Pepper Immunity by Targeting CaWRKY40 against Ralstonia solanacearum Inoculation through Modulating Defense-Related Genes
title_fullStr CaWRKY30 Positively Regulates Pepper Immunity by Targeting CaWRKY40 against Ralstonia solanacearum Inoculation through Modulating Defense-Related Genes
title_full_unstemmed CaWRKY30 Positively Regulates Pepper Immunity by Targeting CaWRKY40 against Ralstonia solanacearum Inoculation through Modulating Defense-Related Genes
title_short CaWRKY30 Positively Regulates Pepper Immunity by Targeting CaWRKY40 against Ralstonia solanacearum Inoculation through Modulating Defense-Related Genes
title_sort cawrky30 positively regulates pepper immunity by targeting cawrky40 against ralstonia solanacearum inoculation through modulating defense-related genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584995/
https://www.ncbi.nlm.nih.gov/pubmed/34769521
http://dx.doi.org/10.3390/ijms222112091
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