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Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence
Leaf senescence is the terminal stage of leaf development. Both light and the plant hormone ethylene play important roles in regulating leaf senescence. However, how they coordinately regulate leaf senescence during leaf development remains largely unclear. In this study, we show that FHY3 and FAR1,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584998/ https://www.ncbi.nlm.nih.gov/pubmed/34777451 http://dx.doi.org/10.3389/fpls.2021.770060 |
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author | Xie, Yurong Ma, Mengdi Liu, Yang Wang, Baobao Wei, Hongbin Kong, Dexin Wang, Haiyang |
author_facet | Xie, Yurong Ma, Mengdi Liu, Yang Wang, Baobao Wei, Hongbin Kong, Dexin Wang, Haiyang |
author_sort | Xie, Yurong |
collection | PubMed |
description | Leaf senescence is the terminal stage of leaf development. Both light and the plant hormone ethylene play important roles in regulating leaf senescence. However, how they coordinately regulate leaf senescence during leaf development remains largely unclear. In this study, we show that FHY3 and FAR1, two homologous proteins essential for phytochrome A-mediated light signaling, physically interact with and repress the DNA binding activity of EIN3 (a key transcription factor essential for ethylene signaling) and PIF5 (a bHLH transcription factor negatively regulating light signaling), and interfere with their DNA binding to the promoter of ORE1, which encodes a key NAC transcription factor promoting leaf senescence. In addition, we show that FHY3, PIF5, and EIN3 form a tri-protein complex(es) and that they coordinately regulate the progression of leaf senescence. We show that during aging or under dark conditions, accumulation of FHY3 protein decreases, thus lifting its repression on DNA binding of EIN3 and PIF5, leading to the increase of ORE1 expression and onset of leaf senescence. Our combined results suggest that FHY3 and FAR1 act in an age gating mechanism to prevent precocious leaf senescence by integrating light and ethylene signaling with developmental aging. |
format | Online Article Text |
id | pubmed-8584998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85849982021-11-12 Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence Xie, Yurong Ma, Mengdi Liu, Yang Wang, Baobao Wei, Hongbin Kong, Dexin Wang, Haiyang Front Plant Sci Plant Science Leaf senescence is the terminal stage of leaf development. Both light and the plant hormone ethylene play important roles in regulating leaf senescence. However, how they coordinately regulate leaf senescence during leaf development remains largely unclear. In this study, we show that FHY3 and FAR1, two homologous proteins essential for phytochrome A-mediated light signaling, physically interact with and repress the DNA binding activity of EIN3 (a key transcription factor essential for ethylene signaling) and PIF5 (a bHLH transcription factor negatively regulating light signaling), and interfere with their DNA binding to the promoter of ORE1, which encodes a key NAC transcription factor promoting leaf senescence. In addition, we show that FHY3, PIF5, and EIN3 form a tri-protein complex(es) and that they coordinately regulate the progression of leaf senescence. We show that during aging or under dark conditions, accumulation of FHY3 protein decreases, thus lifting its repression on DNA binding of EIN3 and PIF5, leading to the increase of ORE1 expression and onset of leaf senescence. Our combined results suggest that FHY3 and FAR1 act in an age gating mechanism to prevent precocious leaf senescence by integrating light and ethylene signaling with developmental aging. Frontiers Media S.A. 2021-10-28 /pmc/articles/PMC8584998/ /pubmed/34777451 http://dx.doi.org/10.3389/fpls.2021.770060 Text en Copyright © 2021 Xie, Ma, Liu, Wang, Wei, Kong and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Xie, Yurong Ma, Mengdi Liu, Yang Wang, Baobao Wei, Hongbin Kong, Dexin Wang, Haiyang Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence |
title | Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence |
title_full | Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence |
title_fullStr | Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence |
title_full_unstemmed | Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence |
title_short | Arabidopsis FHY3 and FAR1 Function in Age Gating of Leaf Senescence |
title_sort | arabidopsis fhy3 and far1 function in age gating of leaf senescence |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8584998/ https://www.ncbi.nlm.nih.gov/pubmed/34777451 http://dx.doi.org/10.3389/fpls.2021.770060 |
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