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AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit

Kiwifruit (Actinidia chinensis Planch) is suitable for neutral acid soil. However, soil salinization is increasing in kiwifruit production areas, which has adverse effects on the growth and development of plants, leading to declining yields and quality. Therefore, analyzing the salt tolerance regula...

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Autores principales: Li, Ming, Wu, Zhiyong, Gu, Hong, Cheng, Dawei, Guo, Xizhi, Li, Lan, Shi, Caiyun, Xu, Guoyi, Gu, Shichao, Abid, Muhammad, Zhong, Yunpeng, Qi, Xiujuan, Chen, Jinyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585034/
https://www.ncbi.nlm.nih.gov/pubmed/34769325
http://dx.doi.org/10.3390/ijms222111897
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author Li, Ming
Wu, Zhiyong
Gu, Hong
Cheng, Dawei
Guo, Xizhi
Li, Lan
Shi, Caiyun
Xu, Guoyi
Gu, Shichao
Abid, Muhammad
Zhong, Yunpeng
Qi, Xiujuan
Chen, Jinyong
author_facet Li, Ming
Wu, Zhiyong
Gu, Hong
Cheng, Dawei
Guo, Xizhi
Li, Lan
Shi, Caiyun
Xu, Guoyi
Gu, Shichao
Abid, Muhammad
Zhong, Yunpeng
Qi, Xiujuan
Chen, Jinyong
author_sort Li, Ming
collection PubMed
description Kiwifruit (Actinidia chinensis Planch) is suitable for neutral acid soil. However, soil salinization is increasing in kiwifruit production areas, which has adverse effects on the growth and development of plants, leading to declining yields and quality. Therefore, analyzing the salt tolerance regulation mechanism can provide a theoretical basis for the industrial application and germplasm improvement of kiwifruit. We identified 120 NAC members and divided them into 13 subfamilies according to phylogenetic analysis. Subsequently, we conducted a comprehensive and systematic analysis based on the conserved motifs, key amino acid residues in the NAC domain, expression patterns, and protein interaction network predictions and screened the candidate gene AvNAC030. In order to study its function, we adopted the method of heterologous expression in Arabidopsis. Compared with the control, the overexpression plants had higher osmotic adjustment ability and improved antioxidant defense mechanism. These results suggest that AvNAC030 plays a positive role in the salt tolerance regulation mechanism in kiwifruit.
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spelling pubmed-85850342021-11-12 AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit Li, Ming Wu, Zhiyong Gu, Hong Cheng, Dawei Guo, Xizhi Li, Lan Shi, Caiyun Xu, Guoyi Gu, Shichao Abid, Muhammad Zhong, Yunpeng Qi, Xiujuan Chen, Jinyong Int J Mol Sci Article Kiwifruit (Actinidia chinensis Planch) is suitable for neutral acid soil. However, soil salinization is increasing in kiwifruit production areas, which has adverse effects on the growth and development of plants, leading to declining yields and quality. Therefore, analyzing the salt tolerance regulation mechanism can provide a theoretical basis for the industrial application and germplasm improvement of kiwifruit. We identified 120 NAC members and divided them into 13 subfamilies according to phylogenetic analysis. Subsequently, we conducted a comprehensive and systematic analysis based on the conserved motifs, key amino acid residues in the NAC domain, expression patterns, and protein interaction network predictions and screened the candidate gene AvNAC030. In order to study its function, we adopted the method of heterologous expression in Arabidopsis. Compared with the control, the overexpression plants had higher osmotic adjustment ability and improved antioxidant defense mechanism. These results suggest that AvNAC030 plays a positive role in the salt tolerance regulation mechanism in kiwifruit. MDPI 2021-11-02 /pmc/articles/PMC8585034/ /pubmed/34769325 http://dx.doi.org/10.3390/ijms222111897 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Ming
Wu, Zhiyong
Gu, Hong
Cheng, Dawei
Guo, Xizhi
Li, Lan
Shi, Caiyun
Xu, Guoyi
Gu, Shichao
Abid, Muhammad
Zhong, Yunpeng
Qi, Xiujuan
Chen, Jinyong
AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit
title AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit
title_full AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit
title_fullStr AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit
title_full_unstemmed AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit
title_short AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit
title_sort avnac030, a nac domain transcription factor, enhances salt stress tolerance in kiwifruit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585034/
https://www.ncbi.nlm.nih.gov/pubmed/34769325
http://dx.doi.org/10.3390/ijms222111897
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