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AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit
Kiwifruit (Actinidia chinensis Planch) is suitable for neutral acid soil. However, soil salinization is increasing in kiwifruit production areas, which has adverse effects on the growth and development of plants, leading to declining yields and quality. Therefore, analyzing the salt tolerance regula...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585034/ https://www.ncbi.nlm.nih.gov/pubmed/34769325 http://dx.doi.org/10.3390/ijms222111897 |
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author | Li, Ming Wu, Zhiyong Gu, Hong Cheng, Dawei Guo, Xizhi Li, Lan Shi, Caiyun Xu, Guoyi Gu, Shichao Abid, Muhammad Zhong, Yunpeng Qi, Xiujuan Chen, Jinyong |
author_facet | Li, Ming Wu, Zhiyong Gu, Hong Cheng, Dawei Guo, Xizhi Li, Lan Shi, Caiyun Xu, Guoyi Gu, Shichao Abid, Muhammad Zhong, Yunpeng Qi, Xiujuan Chen, Jinyong |
author_sort | Li, Ming |
collection | PubMed |
description | Kiwifruit (Actinidia chinensis Planch) is suitable for neutral acid soil. However, soil salinization is increasing in kiwifruit production areas, which has adverse effects on the growth and development of plants, leading to declining yields and quality. Therefore, analyzing the salt tolerance regulation mechanism can provide a theoretical basis for the industrial application and germplasm improvement of kiwifruit. We identified 120 NAC members and divided them into 13 subfamilies according to phylogenetic analysis. Subsequently, we conducted a comprehensive and systematic analysis based on the conserved motifs, key amino acid residues in the NAC domain, expression patterns, and protein interaction network predictions and screened the candidate gene AvNAC030. In order to study its function, we adopted the method of heterologous expression in Arabidopsis. Compared with the control, the overexpression plants had higher osmotic adjustment ability and improved antioxidant defense mechanism. These results suggest that AvNAC030 plays a positive role in the salt tolerance regulation mechanism in kiwifruit. |
format | Online Article Text |
id | pubmed-8585034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85850342021-11-12 AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit Li, Ming Wu, Zhiyong Gu, Hong Cheng, Dawei Guo, Xizhi Li, Lan Shi, Caiyun Xu, Guoyi Gu, Shichao Abid, Muhammad Zhong, Yunpeng Qi, Xiujuan Chen, Jinyong Int J Mol Sci Article Kiwifruit (Actinidia chinensis Planch) is suitable for neutral acid soil. However, soil salinization is increasing in kiwifruit production areas, which has adverse effects on the growth and development of plants, leading to declining yields and quality. Therefore, analyzing the salt tolerance regulation mechanism can provide a theoretical basis for the industrial application and germplasm improvement of kiwifruit. We identified 120 NAC members and divided them into 13 subfamilies according to phylogenetic analysis. Subsequently, we conducted a comprehensive and systematic analysis based on the conserved motifs, key amino acid residues in the NAC domain, expression patterns, and protein interaction network predictions and screened the candidate gene AvNAC030. In order to study its function, we adopted the method of heterologous expression in Arabidopsis. Compared with the control, the overexpression plants had higher osmotic adjustment ability and improved antioxidant defense mechanism. These results suggest that AvNAC030 plays a positive role in the salt tolerance regulation mechanism in kiwifruit. MDPI 2021-11-02 /pmc/articles/PMC8585034/ /pubmed/34769325 http://dx.doi.org/10.3390/ijms222111897 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Ming Wu, Zhiyong Gu, Hong Cheng, Dawei Guo, Xizhi Li, Lan Shi, Caiyun Xu, Guoyi Gu, Shichao Abid, Muhammad Zhong, Yunpeng Qi, Xiujuan Chen, Jinyong AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit |
title | AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit |
title_full | AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit |
title_fullStr | AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit |
title_full_unstemmed | AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit |
title_short | AvNAC030, a NAC Domain Transcription Factor, Enhances Salt Stress Tolerance in Kiwifruit |
title_sort | avnac030, a nac domain transcription factor, enhances salt stress tolerance in kiwifruit |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585034/ https://www.ncbi.nlm.nih.gov/pubmed/34769325 http://dx.doi.org/10.3390/ijms222111897 |
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