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A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma

Melanoma is the deadliest form of skin cancer due to its ability to colonize distant sites and initiate metastasis. Although these processes largely depend on the lipid-based cell membrane scaffold, our understanding of the melanoma lipid phenotype lags behind most other aspects of this tumor cell....

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Autores principales: Perez-Valle, Arantza, Abad-García, Beatriz, Fresnedo, Olatz, Barreda-Gómez, Gabriel, Aspichueta, Patricia, Asumendi, Aintzane, Astigarraga, Egoitz, Fernández, José A., Boyano, María Dolores, Ochoa, Begoña
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585039/
https://www.ncbi.nlm.nih.gov/pubmed/34769491
http://dx.doi.org/10.3390/ijms222112061
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author Perez-Valle, Arantza
Abad-García, Beatriz
Fresnedo, Olatz
Barreda-Gómez, Gabriel
Aspichueta, Patricia
Asumendi, Aintzane
Astigarraga, Egoitz
Fernández, José A.
Boyano, María Dolores
Ochoa, Begoña
author_facet Perez-Valle, Arantza
Abad-García, Beatriz
Fresnedo, Olatz
Barreda-Gómez, Gabriel
Aspichueta, Patricia
Asumendi, Aintzane
Astigarraga, Egoitz
Fernández, José A.
Boyano, María Dolores
Ochoa, Begoña
author_sort Perez-Valle, Arantza
collection PubMed
description Melanoma is the deadliest form of skin cancer due to its ability to colonize distant sites and initiate metastasis. Although these processes largely depend on the lipid-based cell membrane scaffold, our understanding of the melanoma lipid phenotype lags behind most other aspects of this tumor cell. Here, we examined a panel of normal human epidermal and nevus melanocytes and primary and metastatic melanoma cell lines to determine whether distinctive cell-intrinsic lipidomes can discern non-neoplastic from neoplastic melanocytes and define their metastatic potential. Lipidome profiles were obtained by UHPLC-ESI mass-spectrometry, and differences in the signatures were analyzed by multivariate statistical analyses. Significant and highly specific changes in more than 30 lipid species were annotated in the initiation of melanoma, whereas less numerous changes were associated with melanoma progression and the non-malignant transformation of nevus melanocytes. Notably, the “malignancy lipid signature” features marked drops in pivotal membrane lipids, like sphingomyelins, and aberrant elevation of ether-type lipids and phosphatidylglycerol and phosphatidylinositol variants, suggesting a previously undefined remodeling of sphingolipid and glycerophospholipid metabolism. Besides broadening the molecular definition of this neoplasm, the different lipid profiles identified may help improve the clinical diagnosis/prognosis and facilitate therapeutic interventions for cutaneous melanoma.
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spelling pubmed-85850392021-11-12 A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma Perez-Valle, Arantza Abad-García, Beatriz Fresnedo, Olatz Barreda-Gómez, Gabriel Aspichueta, Patricia Asumendi, Aintzane Astigarraga, Egoitz Fernández, José A. Boyano, María Dolores Ochoa, Begoña Int J Mol Sci Article Melanoma is the deadliest form of skin cancer due to its ability to colonize distant sites and initiate metastasis. Although these processes largely depend on the lipid-based cell membrane scaffold, our understanding of the melanoma lipid phenotype lags behind most other aspects of this tumor cell. Here, we examined a panel of normal human epidermal and nevus melanocytes and primary and metastatic melanoma cell lines to determine whether distinctive cell-intrinsic lipidomes can discern non-neoplastic from neoplastic melanocytes and define their metastatic potential. Lipidome profiles were obtained by UHPLC-ESI mass-spectrometry, and differences in the signatures were analyzed by multivariate statistical analyses. Significant and highly specific changes in more than 30 lipid species were annotated in the initiation of melanoma, whereas less numerous changes were associated with melanoma progression and the non-malignant transformation of nevus melanocytes. Notably, the “malignancy lipid signature” features marked drops in pivotal membrane lipids, like sphingomyelins, and aberrant elevation of ether-type lipids and phosphatidylglycerol and phosphatidylinositol variants, suggesting a previously undefined remodeling of sphingolipid and glycerophospholipid metabolism. Besides broadening the molecular definition of this neoplasm, the different lipid profiles identified may help improve the clinical diagnosis/prognosis and facilitate therapeutic interventions for cutaneous melanoma. MDPI 2021-11-08 /pmc/articles/PMC8585039/ /pubmed/34769491 http://dx.doi.org/10.3390/ijms222112061 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Perez-Valle, Arantza
Abad-García, Beatriz
Fresnedo, Olatz
Barreda-Gómez, Gabriel
Aspichueta, Patricia
Asumendi, Aintzane
Astigarraga, Egoitz
Fernández, José A.
Boyano, María Dolores
Ochoa, Begoña
A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma
title A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma
title_full A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma
title_fullStr A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma
title_full_unstemmed A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma
title_short A UHPLC-Mass Spectrometry View of Human Melanocytic Cells Uncovers Potential Lipid Biomarkers of Melanoma
title_sort uhplc-mass spectrometry view of human melanocytic cells uncovers potential lipid biomarkers of melanoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585039/
https://www.ncbi.nlm.nih.gov/pubmed/34769491
http://dx.doi.org/10.3390/ijms222112061
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