Step-by-step preparation of mouse eye sections for routine histology, immunofluorescence, and RNA in situ hybridization multiplexing

It can be challenging to maintain tissue integrity using established histology protocols. Here, we describe a protocol composed of Hartman’s fixation, window technique, microwave-based tissue processing, optimized depigmentation, and antigen retrieval pretreatment. This is followed by the ViewRNA si...

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Detalles Bibliográficos
Autores principales: Pang, Ji, Thomas, Nancy, Tsuchiya, Dai, Parmely, Tari, Yan, Deyue, Xie, Ting, Wang, Yongfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585658/
https://www.ncbi.nlm.nih.gov/pubmed/34806041
http://dx.doi.org/10.1016/j.xpro.2021.100879

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