Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system

Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-...

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Detalles Bibliográficos
Autores principales: Xu, Mei, Zhang, Wenhao, Geng, Mengyang, Zhao, Yiding, Sun, Shengyi, Gao, Qian, Liu, Yan, Shuai, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585661/
https://www.ncbi.nlm.nih.gov/pubmed/34806042
http://dx.doi.org/10.1016/j.xpro.2021.100881
Descripción
Sumario:Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-term and can produce genome-wide mutants combined with transposons. It is promising in high-throughput genetic screening of trophoblast-specific modulators. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019).

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