Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system

Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-...

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Detalles Bibliográficos
Autores principales: Xu, Mei, Zhang, Wenhao, Geng, Mengyang, Zhao, Yiding, Sun, Shengyi, Gao, Qian, Liu, Yan, Shuai, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585661/
https://www.ncbi.nlm.nih.gov/pubmed/34806042
http://dx.doi.org/10.1016/j.xpro.2021.100881
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author Xu, Mei
Zhang, Wenhao
Geng, Mengyang
Zhao, Yiding
Sun, Shengyi
Gao, Qian
Liu, Yan
Shuai, Ling
author_facet Xu, Mei
Zhang, Wenhao
Geng, Mengyang
Zhao, Yiding
Sun, Shengyi
Gao, Qian
Liu, Yan
Shuai, Ling
author_sort Xu, Mei
collection PubMed
description Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-term and can produce genome-wide mutants combined with transposons. It is promising in high-throughput genetic screening of trophoblast-specific modulators. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019).
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spelling pubmed-85856612021-11-18 Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system Xu, Mei Zhang, Wenhao Geng, Mengyang Zhao, Yiding Sun, Shengyi Gao, Qian Liu, Yan Shuai, Ling STAR Protoc Protocol Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-term and can produce genome-wide mutants combined with transposons. It is promising in high-throughput genetic screening of trophoblast-specific modulators. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019). Elsevier 2021-11-05 /pmc/articles/PMC8585661/ /pubmed/34806042 http://dx.doi.org/10.1016/j.xpro.2021.100881 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Xu, Mei
Zhang, Wenhao
Geng, Mengyang
Zhao, Yiding
Sun, Shengyi
Gao, Qian
Liu, Yan
Shuai, Ling
Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system
title Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system
title_full Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system
title_fullStr Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system
title_full_unstemmed Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system
title_short Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system
title_sort rapid generation of murine haploid-induced trophoblast stem cells via a tet-on system
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585661/
https://www.ncbi.nlm.nih.gov/pubmed/34806042
http://dx.doi.org/10.1016/j.xpro.2021.100881
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