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Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment

The nicotine from tobacco stalk showed obvious inhibitory effect on the activity of cellulase and fermentability of microorganisms, which seriously hinders the utilization of tobacco stalk. Dilute sulfuric acid presoak of tobacco stalk was used to enhance the performance of instant catapult steam ex...

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Autores principales: Zhang, Hongsen, Fu, Chenqing, Ren, Tianbao, Xie, Hui, Mao, Guotao, Wang, Zhimin, Wang, Fengqin, Song, Andong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585754/
https://www.ncbi.nlm.nih.gov/pubmed/34778234
http://dx.doi.org/10.3389/fbioe.2021.763549
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author Zhang, Hongsen
Fu, Chenqing
Ren, Tianbao
Xie, Hui
Mao, Guotao
Wang, Zhimin
Wang, Fengqin
Song, Andong
author_facet Zhang, Hongsen
Fu, Chenqing
Ren, Tianbao
Xie, Hui
Mao, Guotao
Wang, Zhimin
Wang, Fengqin
Song, Andong
author_sort Zhang, Hongsen
collection PubMed
description The nicotine from tobacco stalk showed obvious inhibitory effect on the activity of cellulase and fermentability of microorganisms, which seriously hinders the utilization of tobacco stalk. Dilute sulfuric acid presoak of tobacco stalk was used to enhance the performance of instant catapult steam explosion (ICSE) for tobacco stalk pretreatment. The presoak was beneficial to break the recalcitrant structure of tobacco stalk, reduce nicotine content to relieve the inhibition on the activity of cellulase and metabolism of microorganisms, and promote the performance of enzymatic hydrolysis and ethanol fermentation. The optimized 0.8% sulfuric acid (w/w) presoak–integrated ICSE pretreatment resulted in 85.54% nicotine removal from tobacco stalk; meanwhile, the total sugar concentration from enzymatic hydrolysis of pretreated tobacco stalk increased from 33.40 to 53.81 g/L (the ratio of dry tobacco stalk to water was 1:8, w/w), ethanol concentration increased 103.36% from 5.95 to 12.10 g/L in flask, compared with separate ICSE pretreatment. Finally, the ethanol concentration achieved the highest 23.53 g/L in a 5-L fermenter with the ethanol yield from the glucose of tobacco stalk hydrolysate achieving 71.40% by increasing the solid loading of the tobacco stalk in the enzymatic hydrolysis process (the ratio of dry tobacco stalk to water was 1:4, w/w). These results achieved the expected purpose of efficient utilization of discarded tobacco stalk.
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spelling pubmed-85857542021-11-13 Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment Zhang, Hongsen Fu, Chenqing Ren, Tianbao Xie, Hui Mao, Guotao Wang, Zhimin Wang, Fengqin Song, Andong Front Bioeng Biotechnol Bioengineering and Biotechnology The nicotine from tobacco stalk showed obvious inhibitory effect on the activity of cellulase and fermentability of microorganisms, which seriously hinders the utilization of tobacco stalk. Dilute sulfuric acid presoak of tobacco stalk was used to enhance the performance of instant catapult steam explosion (ICSE) for tobacco stalk pretreatment. The presoak was beneficial to break the recalcitrant structure of tobacco stalk, reduce nicotine content to relieve the inhibition on the activity of cellulase and metabolism of microorganisms, and promote the performance of enzymatic hydrolysis and ethanol fermentation. The optimized 0.8% sulfuric acid (w/w) presoak–integrated ICSE pretreatment resulted in 85.54% nicotine removal from tobacco stalk; meanwhile, the total sugar concentration from enzymatic hydrolysis of pretreated tobacco stalk increased from 33.40 to 53.81 g/L (the ratio of dry tobacco stalk to water was 1:8, w/w), ethanol concentration increased 103.36% from 5.95 to 12.10 g/L in flask, compared with separate ICSE pretreatment. Finally, the ethanol concentration achieved the highest 23.53 g/L in a 5-L fermenter with the ethanol yield from the glucose of tobacco stalk hydrolysate achieving 71.40% by increasing the solid loading of the tobacco stalk in the enzymatic hydrolysis process (the ratio of dry tobacco stalk to water was 1:4, w/w). These results achieved the expected purpose of efficient utilization of discarded tobacco stalk. Frontiers Media S.A. 2021-10-29 /pmc/articles/PMC8585754/ /pubmed/34778234 http://dx.doi.org/10.3389/fbioe.2021.763549 Text en Copyright © 2021 Zhang, Fu, Ren, Xie, Mao, Wang, Wang and Song. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zhang, Hongsen
Fu, Chenqing
Ren, Tianbao
Xie, Hui
Mao, Guotao
Wang, Zhimin
Wang, Fengqin
Song, Andong
Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment
title Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment
title_full Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment
title_fullStr Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment
title_full_unstemmed Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment
title_short Improvement of Nicotine Removal and Ethanol Fermentability From Tobacco Stalk by Integration of Dilute Sulfuric Acid Presoak and Instant Catapult Steam Explosion Pretreatment
title_sort improvement of nicotine removal and ethanol fermentability from tobacco stalk by integration of dilute sulfuric acid presoak and instant catapult steam explosion pretreatment
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585754/
https://www.ncbi.nlm.nih.gov/pubmed/34778234
http://dx.doi.org/10.3389/fbioe.2021.763549
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