Cargando…

RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation

Given the importance of wood in many industrial applications, much research has focused on wood formation, especially lignin biosynthesis. However, the mechanisms governing the regulation of lignin biosynthesis in the rubber tree (Hevea brasiliensis) remain to be elucidated. Here, we gained insight...

Descripción completa

Detalles Bibliográficos
Autores principales: Meng, Xiangxu, Wang, Yue, Li, Jia, Jiao, Nanbo, Zhang, Xiujie, Zhang, Yuanyuan, Chen, Jinhui, Tu, Zhihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585928/
https://www.ncbi.nlm.nih.gov/pubmed/34777481
http://dx.doi.org/10.3389/fgene.2021.763841
_version_ 1784597788823126016
author Meng, Xiangxu
Wang, Yue
Li, Jia
Jiao, Nanbo
Zhang, Xiujie
Zhang, Yuanyuan
Chen, Jinhui
Tu, Zhihua
author_facet Meng, Xiangxu
Wang, Yue
Li, Jia
Jiao, Nanbo
Zhang, Xiujie
Zhang, Yuanyuan
Chen, Jinhui
Tu, Zhihua
author_sort Meng, Xiangxu
collection PubMed
description Given the importance of wood in many industrial applications, much research has focused on wood formation, especially lignin biosynthesis. However, the mechanisms governing the regulation of lignin biosynthesis in the rubber tree (Hevea brasiliensis) remain to be elucidated. Here, we gained insight into the mechanisms of rubber tree lignin biosynthesis using reaction wood (wood with abnormal tissue structure induced by gravity or artificial mechanical treatment) as an experimental model. We performed transcriptome analysis of rubber tree mature xylem from tension wood (TW), opposite wood (OW), and normal wood (NW) using RNA sequencing (RNA-seq). A total of 214, 1,280, and 32 differentially expressed genes (DEGs) were identified in TW vs. NW, OW vs. NW, and TW vs. OW, respectively. GO and KEGG enrichment analysis of DEGs from different comparison groups showed that zeatin biosynthesis, plant hormone signal transduction, phenylpropanoid biosynthesis, and plant–pathogen interaction pathways may play important roles in reaction wood formation. Sixteen transcripts involved in phenylpropanoid biosynthesis and 129 transcripts encoding transcription factors (TFs) were used to construct a TF–gene regulatory network for rubber tree lignin biosynthesis. Among them, MYB, C2H2, and NAC TFs could regulate all the DEGs involved in phenylpropanoid biosynthesis. Overall, this study identified candidate genes and TFs likely involved in phenylpropanoid biosynthesis and provides novel insights into the mechanisms regulating rubber tree lignin biosynthesis.
format Online
Article
Text
id pubmed-8585928
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-85859282021-11-13 RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation Meng, Xiangxu Wang, Yue Li, Jia Jiao, Nanbo Zhang, Xiujie Zhang, Yuanyuan Chen, Jinhui Tu, Zhihua Front Genet Genetics Given the importance of wood in many industrial applications, much research has focused on wood formation, especially lignin biosynthesis. However, the mechanisms governing the regulation of lignin biosynthesis in the rubber tree (Hevea brasiliensis) remain to be elucidated. Here, we gained insight into the mechanisms of rubber tree lignin biosynthesis using reaction wood (wood with abnormal tissue structure induced by gravity or artificial mechanical treatment) as an experimental model. We performed transcriptome analysis of rubber tree mature xylem from tension wood (TW), opposite wood (OW), and normal wood (NW) using RNA sequencing (RNA-seq). A total of 214, 1,280, and 32 differentially expressed genes (DEGs) were identified in TW vs. NW, OW vs. NW, and TW vs. OW, respectively. GO and KEGG enrichment analysis of DEGs from different comparison groups showed that zeatin biosynthesis, plant hormone signal transduction, phenylpropanoid biosynthesis, and plant–pathogen interaction pathways may play important roles in reaction wood formation. Sixteen transcripts involved in phenylpropanoid biosynthesis and 129 transcripts encoding transcription factors (TFs) were used to construct a TF–gene regulatory network for rubber tree lignin biosynthesis. Among them, MYB, C2H2, and NAC TFs could regulate all the DEGs involved in phenylpropanoid biosynthesis. Overall, this study identified candidate genes and TFs likely involved in phenylpropanoid biosynthesis and provides novel insights into the mechanisms regulating rubber tree lignin biosynthesis. Frontiers Media S.A. 2021-10-29 /pmc/articles/PMC8585928/ /pubmed/34777481 http://dx.doi.org/10.3389/fgene.2021.763841 Text en Copyright © 2021 Meng, Wang, Li, Jiao, Zhang, Zhang, Chen and Tu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Meng, Xiangxu
Wang, Yue
Li, Jia
Jiao, Nanbo
Zhang, Xiujie
Zhang, Yuanyuan
Chen, Jinhui
Tu, Zhihua
RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation
title RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation
title_full RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation
title_fullStr RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation
title_full_unstemmed RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation
title_short RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors for Hevea brasiliensis Reaction Wood Formation
title_sort rna sequencing reveals phenylpropanoid biosynthesis genes and transcription factors for hevea brasiliensis reaction wood formation
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585928/
https://www.ncbi.nlm.nih.gov/pubmed/34777481
http://dx.doi.org/10.3389/fgene.2021.763841
work_keys_str_mv AT mengxiangxu rnasequencingrevealsphenylpropanoidbiosynthesisgenesandtranscriptionfactorsforheveabrasiliensisreactionwoodformation
AT wangyue rnasequencingrevealsphenylpropanoidbiosynthesisgenesandtranscriptionfactorsforheveabrasiliensisreactionwoodformation
AT lijia rnasequencingrevealsphenylpropanoidbiosynthesisgenesandtranscriptionfactorsforheveabrasiliensisreactionwoodformation
AT jiaonanbo rnasequencingrevealsphenylpropanoidbiosynthesisgenesandtranscriptionfactorsforheveabrasiliensisreactionwoodformation
AT zhangxiujie rnasequencingrevealsphenylpropanoidbiosynthesisgenesandtranscriptionfactorsforheveabrasiliensisreactionwoodformation
AT zhangyuanyuan rnasequencingrevealsphenylpropanoidbiosynthesisgenesandtranscriptionfactorsforheveabrasiliensisreactionwoodformation
AT chenjinhui rnasequencingrevealsphenylpropanoidbiosynthesisgenesandtranscriptionfactorsforheveabrasiliensisreactionwoodformation
AT tuzhihua rnasequencingrevealsphenylpropanoidbiosynthesisgenesandtranscriptionfactorsforheveabrasiliensisreactionwoodformation