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Post-acquisition CO(2) Inhalation Enhances Fear Memory and Depends on ASIC1A

A growing body of evidence suggests that memories of fearful events may be altered after initial acquisition or learning. Although much of this work has been done in rodents using Pavlovian fear conditioning, it may have important implications for fear memories in humans such as in post-traumatic st...

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Autores principales: Taugher, Rebecca J., Wunsch, Amanda M., Wang, Grace Z., Chan, Aubrey C., Dlouhy, Brian J., Wemmie, John A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585996/
https://www.ncbi.nlm.nih.gov/pubmed/34776896
http://dx.doi.org/10.3389/fnbeh.2021.767426
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author Taugher, Rebecca J.
Wunsch, Amanda M.
Wang, Grace Z.
Chan, Aubrey C.
Dlouhy, Brian J.
Wemmie, John A.
author_facet Taugher, Rebecca J.
Wunsch, Amanda M.
Wang, Grace Z.
Chan, Aubrey C.
Dlouhy, Brian J.
Wemmie, John A.
author_sort Taugher, Rebecca J.
collection PubMed
description A growing body of evidence suggests that memories of fearful events may be altered after initial acquisition or learning. Although much of this work has been done in rodents using Pavlovian fear conditioning, it may have important implications for fear memories in humans such as in post-traumatic stress disorder (PTSD). A recent study suggested that cued fear memories, made labile by memory retrieval, were made additionally labile and thus more vulnerable to subsequent modification when mice inhaled 10% carbon dioxide (CO(2)) during retrieval. In light of this finding, we hypothesized that 10% CO(2) inhalation soon after fear acquisition might affect memory recall 24 h later. We found that both cue and context fear memory were increased by CO(2) exposure after fear acquisition. The effect of CO(2) was time-dependent, as CO(2) inhalation administered 1 or 4 h after cued fear acquisition increased fear memory, whereas CO(2) inhalation 4 h before or 24 h after cued fear acquisition did not increase fear memory. The ability of CO(2) exposure following acquisition to enhance fear memory was not a general consequence of stress, as restraining mice after acquisition did not alter cued fear memory. The memory-enhancing action of CO(2) may be relatively specific to fear conditioning as novel object recognition was impaired by post-training CO(2) inhalation. To explore the molecular underpinnings of these effects, we tested if they depended on the acid-sensing ion channel-1a (ASIC1A), a proton-gated cation channel that mediates other effects of CO(2), likely via its ability to sense acidosis induced during CO(2) inhalation. We found that CO(2) inhalation did not alter cued or context fear memory in Asic1a(–/–) mice, suggesting that this phenomenon critically depends on ASIC1A. These results suggest that brain acidosis around the time of a traumatic event may enhance memory of the trauma, and may thus constitute an important risk factor for developing PTSD. Moreover, preventing peritraumatic acidosis might reduce risk of PTSD.
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spelling pubmed-85859962021-11-13 Post-acquisition CO(2) Inhalation Enhances Fear Memory and Depends on ASIC1A Taugher, Rebecca J. Wunsch, Amanda M. Wang, Grace Z. Chan, Aubrey C. Dlouhy, Brian J. Wemmie, John A. Front Behav Neurosci Neuroscience A growing body of evidence suggests that memories of fearful events may be altered after initial acquisition or learning. Although much of this work has been done in rodents using Pavlovian fear conditioning, it may have important implications for fear memories in humans such as in post-traumatic stress disorder (PTSD). A recent study suggested that cued fear memories, made labile by memory retrieval, were made additionally labile and thus more vulnerable to subsequent modification when mice inhaled 10% carbon dioxide (CO(2)) during retrieval. In light of this finding, we hypothesized that 10% CO(2) inhalation soon after fear acquisition might affect memory recall 24 h later. We found that both cue and context fear memory were increased by CO(2) exposure after fear acquisition. The effect of CO(2) was time-dependent, as CO(2) inhalation administered 1 or 4 h after cued fear acquisition increased fear memory, whereas CO(2) inhalation 4 h before or 24 h after cued fear acquisition did not increase fear memory. The ability of CO(2) exposure following acquisition to enhance fear memory was not a general consequence of stress, as restraining mice after acquisition did not alter cued fear memory. The memory-enhancing action of CO(2) may be relatively specific to fear conditioning as novel object recognition was impaired by post-training CO(2) inhalation. To explore the molecular underpinnings of these effects, we tested if they depended on the acid-sensing ion channel-1a (ASIC1A), a proton-gated cation channel that mediates other effects of CO(2), likely via its ability to sense acidosis induced during CO(2) inhalation. We found that CO(2) inhalation did not alter cued or context fear memory in Asic1a(–/–) mice, suggesting that this phenomenon critically depends on ASIC1A. These results suggest that brain acidosis around the time of a traumatic event may enhance memory of the trauma, and may thus constitute an important risk factor for developing PTSD. Moreover, preventing peritraumatic acidosis might reduce risk of PTSD. Frontiers Media S.A. 2021-10-29 /pmc/articles/PMC8585996/ /pubmed/34776896 http://dx.doi.org/10.3389/fnbeh.2021.767426 Text en Copyright © 2021 Taugher, Wunsch, Wang, Chan, Dlouhy and Wemmie. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Taugher, Rebecca J.
Wunsch, Amanda M.
Wang, Grace Z.
Chan, Aubrey C.
Dlouhy, Brian J.
Wemmie, John A.
Post-acquisition CO(2) Inhalation Enhances Fear Memory and Depends on ASIC1A
title Post-acquisition CO(2) Inhalation Enhances Fear Memory and Depends on ASIC1A
title_full Post-acquisition CO(2) Inhalation Enhances Fear Memory and Depends on ASIC1A
title_fullStr Post-acquisition CO(2) Inhalation Enhances Fear Memory and Depends on ASIC1A
title_full_unstemmed Post-acquisition CO(2) Inhalation Enhances Fear Memory and Depends on ASIC1A
title_short Post-acquisition CO(2) Inhalation Enhances Fear Memory and Depends on ASIC1A
title_sort post-acquisition co(2) inhalation enhances fear memory and depends on asic1a
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585996/
https://www.ncbi.nlm.nih.gov/pubmed/34776896
http://dx.doi.org/10.3389/fnbeh.2021.767426
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