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Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values

Diutina catenulata (Candida catenulata) is an ascomycete yeast species widely used in environmental and industrial research and capable of causing infections in humans and animals. At present, there are only a few studies on D. catenulata, and further research is required for its more in-depth chara...

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Autores principales: Chen, Xin-Fei, Zhang, Wei, Fan, Xin, Hou, Xin, Liu, Xiao-Yu, Huang, Jing-Jing, Kang, Wei, Zhang, Ge, Zhang, Han, Yang, Wen-Hang, Li, Ying-Xing, Wang, Jin-Wen, Guo, Da-Wen, Sun, Zi-Yong, Chen, Zhong-Ju, Zou, Ling-Gui, Du, Xue-Fei, Pan, Yu-Hong, Li, Bin, He, Hong, Xu, Ying-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586209/
https://www.ncbi.nlm.nih.gov/pubmed/34778103
http://dx.doi.org/10.3389/fcimb.2021.739496
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author Chen, Xin-Fei
Zhang, Wei
Fan, Xin
Hou, Xin
Liu, Xiao-Yu
Huang, Jing-Jing
Kang, Wei
Zhang, Ge
Zhang, Han
Yang, Wen-Hang
Li, Ying-Xing
Wang, Jin-Wen
Guo, Da-Wen
Sun, Zi-Yong
Chen, Zhong-Ju
Zou, Ling-Gui
Du, Xue-Fei
Pan, Yu-Hong
Li, Bin
He, Hong
Xu, Ying-Chun
author_facet Chen, Xin-Fei
Zhang, Wei
Fan, Xin
Hou, Xin
Liu, Xiao-Yu
Huang, Jing-Jing
Kang, Wei
Zhang, Ge
Zhang, Han
Yang, Wen-Hang
Li, Ying-Xing
Wang, Jin-Wen
Guo, Da-Wen
Sun, Zi-Yong
Chen, Zhong-Ju
Zou, Ling-Gui
Du, Xue-Fei
Pan, Yu-Hong
Li, Bin
He, Hong
Xu, Ying-Chun
author_sort Chen, Xin-Fei
collection PubMed
description Diutina catenulata (Candida catenulata) is an ascomycete yeast species widely used in environmental and industrial research and capable of causing infections in humans and animals. At present, there are only a few studies on D. catenulata, and further research is required for its more in-depth characterization and analysis. Eleven strains of D. catenulata collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and the CHIF-NET North China Program were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry and internal transcribed spacer sequencing. The antifungal susceptibility of the Diutina catenulata strains was tested using the Clinical and Laboratory Standards Institute broth microdilution method and Sensititre YeastOne™. Furthermore, ERG11 and FKS1 were sequenced to determine any mutations related to azole and echinocandin resistance in D. catenulata. All isolates exhibited low minimum inhibitory concentration (MIC) values for itraconazole (0.06–0.12 μg/ml), posaconazole (0.06–0.12 μg/ml), amphotericin B (0.25–1 μg/ml), and 5-flucytosine (range, <0.06–0.12 μg/ml), whereas four isolates showed high MICs (≥4 μg/ml) for echinocandins. Strains with high MIC values for azoles showed common ERG11 mutations, namely, F126L/K143R. In addition, L139R mutations may be linked to high MICs of fluconazole. Two amino acid alterations reported to correspond to high MIC values of echinocandin, namely, F621I (F641) and S625L (S645), were found in the hot spot 1 region of FKS1. In addition, one new amino acid alteration, I1348S (I1368), was found outside of the FKS1 hot spot 2 region, and its contribution to echinocandin resistance requires future investigation. Diutina catenulata mainly infects patients with a weak immune system, and the high MIC values for various antifungals exhibited by these isolates may represent a challenge to clinical treatment.
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spelling pubmed-85862092021-11-13 Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values Chen, Xin-Fei Zhang, Wei Fan, Xin Hou, Xin Liu, Xiao-Yu Huang, Jing-Jing Kang, Wei Zhang, Ge Zhang, Han Yang, Wen-Hang Li, Ying-Xing Wang, Jin-Wen Guo, Da-Wen Sun, Zi-Yong Chen, Zhong-Ju Zou, Ling-Gui Du, Xue-Fei Pan, Yu-Hong Li, Bin He, Hong Xu, Ying-Chun Front Cell Infect Microbiol Cellular and Infection Microbiology Diutina catenulata (Candida catenulata) is an ascomycete yeast species widely used in environmental and industrial research and capable of causing infections in humans and animals. At present, there are only a few studies on D. catenulata, and further research is required for its more in-depth characterization and analysis. Eleven strains of D. catenulata collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and the CHIF-NET North China Program were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry and internal transcribed spacer sequencing. The antifungal susceptibility of the Diutina catenulata strains was tested using the Clinical and Laboratory Standards Institute broth microdilution method and Sensititre YeastOne™. Furthermore, ERG11 and FKS1 were sequenced to determine any mutations related to azole and echinocandin resistance in D. catenulata. All isolates exhibited low minimum inhibitory concentration (MIC) values for itraconazole (0.06–0.12 μg/ml), posaconazole (0.06–0.12 μg/ml), amphotericin B (0.25–1 μg/ml), and 5-flucytosine (range, <0.06–0.12 μg/ml), whereas four isolates showed high MICs (≥4 μg/ml) for echinocandins. Strains with high MIC values for azoles showed common ERG11 mutations, namely, F126L/K143R. In addition, L139R mutations may be linked to high MICs of fluconazole. Two amino acid alterations reported to correspond to high MIC values of echinocandin, namely, F621I (F641) and S625L (S645), were found in the hot spot 1 region of FKS1. In addition, one new amino acid alteration, I1348S (I1368), was found outside of the FKS1 hot spot 2 region, and its contribution to echinocandin resistance requires future investigation. Diutina catenulata mainly infects patients with a weak immune system, and the high MIC values for various antifungals exhibited by these isolates may represent a challenge to clinical treatment. Frontiers Media S.A. 2021-10-29 /pmc/articles/PMC8586209/ /pubmed/34778103 http://dx.doi.org/10.3389/fcimb.2021.739496 Text en Copyright © 2021 Chen, Zhang, Fan, Hou, Liu, Huang, Kang, Zhang, Zhang, Yang, Li, Wang, Guo, Sun, Chen, Zou, Du, Pan, Li, He and Xu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Chen, Xin-Fei
Zhang, Wei
Fan, Xin
Hou, Xin
Liu, Xiao-Yu
Huang, Jing-Jing
Kang, Wei
Zhang, Ge
Zhang, Han
Yang, Wen-Hang
Li, Ying-Xing
Wang, Jin-Wen
Guo, Da-Wen
Sun, Zi-Yong
Chen, Zhong-Ju
Zou, Ling-Gui
Du, Xue-Fei
Pan, Yu-Hong
Li, Bin
He, Hong
Xu, Ying-Chun
Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values
title Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values
title_full Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values
title_fullStr Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values
title_full_unstemmed Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values
title_short Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values
title_sort antifungal susceptibility profiles and resistance mechanisms of clinical diutina catenulata isolates with high mic values
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586209/
https://www.ncbi.nlm.nih.gov/pubmed/34778103
http://dx.doi.org/10.3389/fcimb.2021.739496
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