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Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values
Diutina catenulata (Candida catenulata) is an ascomycete yeast species widely used in environmental and industrial research and capable of causing infections in humans and animals. At present, there are only a few studies on D. catenulata, and further research is required for its more in-depth chara...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586209/ https://www.ncbi.nlm.nih.gov/pubmed/34778103 http://dx.doi.org/10.3389/fcimb.2021.739496 |
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author | Chen, Xin-Fei Zhang, Wei Fan, Xin Hou, Xin Liu, Xiao-Yu Huang, Jing-Jing Kang, Wei Zhang, Ge Zhang, Han Yang, Wen-Hang Li, Ying-Xing Wang, Jin-Wen Guo, Da-Wen Sun, Zi-Yong Chen, Zhong-Ju Zou, Ling-Gui Du, Xue-Fei Pan, Yu-Hong Li, Bin He, Hong Xu, Ying-Chun |
author_facet | Chen, Xin-Fei Zhang, Wei Fan, Xin Hou, Xin Liu, Xiao-Yu Huang, Jing-Jing Kang, Wei Zhang, Ge Zhang, Han Yang, Wen-Hang Li, Ying-Xing Wang, Jin-Wen Guo, Da-Wen Sun, Zi-Yong Chen, Zhong-Ju Zou, Ling-Gui Du, Xue-Fei Pan, Yu-Hong Li, Bin He, Hong Xu, Ying-Chun |
author_sort | Chen, Xin-Fei |
collection | PubMed |
description | Diutina catenulata (Candida catenulata) is an ascomycete yeast species widely used in environmental and industrial research and capable of causing infections in humans and animals. At present, there are only a few studies on D. catenulata, and further research is required for its more in-depth characterization and analysis. Eleven strains of D. catenulata collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and the CHIF-NET North China Program were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry and internal transcribed spacer sequencing. The antifungal susceptibility of the Diutina catenulata strains was tested using the Clinical and Laboratory Standards Institute broth microdilution method and Sensititre YeastOne™. Furthermore, ERG11 and FKS1 were sequenced to determine any mutations related to azole and echinocandin resistance in D. catenulata. All isolates exhibited low minimum inhibitory concentration (MIC) values for itraconazole (0.06–0.12 μg/ml), posaconazole (0.06–0.12 μg/ml), amphotericin B (0.25–1 μg/ml), and 5-flucytosine (range, <0.06–0.12 μg/ml), whereas four isolates showed high MICs (≥4 μg/ml) for echinocandins. Strains with high MIC values for azoles showed common ERG11 mutations, namely, F126L/K143R. In addition, L139R mutations may be linked to high MICs of fluconazole. Two amino acid alterations reported to correspond to high MIC values of echinocandin, namely, F621I (F641) and S625L (S645), were found in the hot spot 1 region of FKS1. In addition, one new amino acid alteration, I1348S (I1368), was found outside of the FKS1 hot spot 2 region, and its contribution to echinocandin resistance requires future investigation. Diutina catenulata mainly infects patients with a weak immune system, and the high MIC values for various antifungals exhibited by these isolates may represent a challenge to clinical treatment. |
format | Online Article Text |
id | pubmed-8586209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85862092021-11-13 Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values Chen, Xin-Fei Zhang, Wei Fan, Xin Hou, Xin Liu, Xiao-Yu Huang, Jing-Jing Kang, Wei Zhang, Ge Zhang, Han Yang, Wen-Hang Li, Ying-Xing Wang, Jin-Wen Guo, Da-Wen Sun, Zi-Yong Chen, Zhong-Ju Zou, Ling-Gui Du, Xue-Fei Pan, Yu-Hong Li, Bin He, Hong Xu, Ying-Chun Front Cell Infect Microbiol Cellular and Infection Microbiology Diutina catenulata (Candida catenulata) is an ascomycete yeast species widely used in environmental and industrial research and capable of causing infections in humans and animals. At present, there are only a few studies on D. catenulata, and further research is required for its more in-depth characterization and analysis. Eleven strains of D. catenulata collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and the CHIF-NET North China Program were identified using matrix-assisted laser desorption ionization–time of flight mass spectrometry and internal transcribed spacer sequencing. The antifungal susceptibility of the Diutina catenulata strains was tested using the Clinical and Laboratory Standards Institute broth microdilution method and Sensititre YeastOne™. Furthermore, ERG11 and FKS1 were sequenced to determine any mutations related to azole and echinocandin resistance in D. catenulata. All isolates exhibited low minimum inhibitory concentration (MIC) values for itraconazole (0.06–0.12 μg/ml), posaconazole (0.06–0.12 μg/ml), amphotericin B (0.25–1 μg/ml), and 5-flucytosine (range, <0.06–0.12 μg/ml), whereas four isolates showed high MICs (≥4 μg/ml) for echinocandins. Strains with high MIC values for azoles showed common ERG11 mutations, namely, F126L/K143R. In addition, L139R mutations may be linked to high MICs of fluconazole. Two amino acid alterations reported to correspond to high MIC values of echinocandin, namely, F621I (F641) and S625L (S645), were found in the hot spot 1 region of FKS1. In addition, one new amino acid alteration, I1348S (I1368), was found outside of the FKS1 hot spot 2 region, and its contribution to echinocandin resistance requires future investigation. Diutina catenulata mainly infects patients with a weak immune system, and the high MIC values for various antifungals exhibited by these isolates may represent a challenge to clinical treatment. Frontiers Media S.A. 2021-10-29 /pmc/articles/PMC8586209/ /pubmed/34778103 http://dx.doi.org/10.3389/fcimb.2021.739496 Text en Copyright © 2021 Chen, Zhang, Fan, Hou, Liu, Huang, Kang, Zhang, Zhang, Yang, Li, Wang, Guo, Sun, Chen, Zou, Du, Pan, Li, He and Xu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Chen, Xin-Fei Zhang, Wei Fan, Xin Hou, Xin Liu, Xiao-Yu Huang, Jing-Jing Kang, Wei Zhang, Ge Zhang, Han Yang, Wen-Hang Li, Ying-Xing Wang, Jin-Wen Guo, Da-Wen Sun, Zi-Yong Chen, Zhong-Ju Zou, Ling-Gui Du, Xue-Fei Pan, Yu-Hong Li, Bin He, Hong Xu, Ying-Chun Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values |
title | Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values |
title_full | Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values |
title_fullStr | Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values |
title_full_unstemmed | Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values |
title_short | Antifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Diutina catenulata Isolates With High MIC Values |
title_sort | antifungal susceptibility profiles and resistance mechanisms of clinical diutina catenulata isolates with high mic values |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586209/ https://www.ncbi.nlm.nih.gov/pubmed/34778103 http://dx.doi.org/10.3389/fcimb.2021.739496 |
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