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Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice
The cholinergic efferent network from the medial septal nucleus to the hippocampus plays an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to encode novel information. Hippocampal cholinergic neurostimulating peptide (H...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586363/ https://www.ncbi.nlm.nih.gov/pubmed/34764402 http://dx.doi.org/10.1038/s41598-021-01667-8 |
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author | Kondo-Takuma, Yuko Mizuno, Masayuki Tsuda, Yo Madokoro, Yuta Suzuki, Kengo Sato, Toyohiro Takase, Hiroshi Uchida, Yuto Adachi, Ken-ichi Hida, Hideki Borlongan, Cesario V. Matsukawa, Noriyuki |
author_facet | Kondo-Takuma, Yuko Mizuno, Masayuki Tsuda, Yo Madokoro, Yuta Suzuki, Kengo Sato, Toyohiro Takase, Hiroshi Uchida, Yuto Adachi, Ken-ichi Hida, Hideki Borlongan, Cesario V. Matsukawa, Noriyuki |
author_sort | Kondo-Takuma, Yuko |
collection | PubMed |
description | The cholinergic efferent network from the medial septal nucleus to the hippocampus plays an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP), which induces acetylcholine (Ach) synthesis in the medial septal nuclei of an explant culture system, was purified from the soluble fraction of postnatal rat hippocampus. HCNP is processed from the N-terminal region of a 186-amino acid, 21-kDa HCNP precursor protein, also known as Raf kinase inhibitory protein and phosphatidylethanolamine-binding protein 1. Here, we confirmed direct reduction of Ach release in the hippocampus of freely moving HCNP-pp knockout mice under an arousal state by the microdialysis method. The levels of vesicular acetylcholine transporter were also decreased in the hippocampus of these mice in comparison with those in control mice, suggesting there was decreased incorporation of Ach into the synaptic vesicle. These results potently indicate that HCNP may be a cholinergic regulator in the septo-hippocampal network. |
format | Online Article Text |
id | pubmed-8586363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85863632021-11-16 Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice Kondo-Takuma, Yuko Mizuno, Masayuki Tsuda, Yo Madokoro, Yuta Suzuki, Kengo Sato, Toyohiro Takase, Hiroshi Uchida, Yuto Adachi, Ken-ichi Hida, Hideki Borlongan, Cesario V. Matsukawa, Noriyuki Sci Rep Article The cholinergic efferent network from the medial septal nucleus to the hippocampus plays an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP), which induces acetylcholine (Ach) synthesis in the medial septal nuclei of an explant culture system, was purified from the soluble fraction of postnatal rat hippocampus. HCNP is processed from the N-terminal region of a 186-amino acid, 21-kDa HCNP precursor protein, also known as Raf kinase inhibitory protein and phosphatidylethanolamine-binding protein 1. Here, we confirmed direct reduction of Ach release in the hippocampus of freely moving HCNP-pp knockout mice under an arousal state by the microdialysis method. The levels of vesicular acetylcholine transporter were also decreased in the hippocampus of these mice in comparison with those in control mice, suggesting there was decreased incorporation of Ach into the synaptic vesicle. These results potently indicate that HCNP may be a cholinergic regulator in the septo-hippocampal network. Nature Publishing Group UK 2021-11-11 /pmc/articles/PMC8586363/ /pubmed/34764402 http://dx.doi.org/10.1038/s41598-021-01667-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kondo-Takuma, Yuko Mizuno, Masayuki Tsuda, Yo Madokoro, Yuta Suzuki, Kengo Sato, Toyohiro Takase, Hiroshi Uchida, Yuto Adachi, Ken-ichi Hida, Hideki Borlongan, Cesario V. Matsukawa, Noriyuki Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice |
title | Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice |
title_full | Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice |
title_fullStr | Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice |
title_full_unstemmed | Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice |
title_short | Reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice |
title_sort | reduction of acetylcholine in the hippocampus of hippocampal cholinergic neurostimulating peptide precursor protein knockout mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586363/ https://www.ncbi.nlm.nih.gov/pubmed/34764402 http://dx.doi.org/10.1038/s41598-021-01667-8 |
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