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Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions
Purpose: To investigate the effects of exogenous basic fibroblast growth factor -2 (FGF-2) on the biological activity of endothelial progenitor cells (EPCs) exposed to high glucose conditions. Materials and Methods: 1) Bone marrow EPCs from C57BL/6 mice were isolated and cultured in vitro. EPC purit...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shanghai Journal of Interventional Radiology Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586578/ https://www.ncbi.nlm.nih.gov/pubmed/34805825 http://dx.doi.org/10.19779/j.cnki.2096-3602.2018.01.04 |
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author | Li, Yang Li, Xue Han, Shilong Lian, Weishuai Cheng, Jie Xie, Xiaoyun Li, Maoquan |
author_facet | Li, Yang Li, Xue Han, Shilong Lian, Weishuai Cheng, Jie Xie, Xiaoyun Li, Maoquan |
author_sort | Li, Yang |
collection | PubMed |
description | Purpose: To investigate the effects of exogenous basic fibroblast growth factor -2 (FGF-2) on the biological activity of endothelial progenitor cells (EPCs) exposed to high glucose conditions. Materials and Methods: 1) Bone marrow EPCs from C57BL/6 mice were isolated and cultured in vitro. EPC purity was identified by flow cytometry and immunofluorescence staining. 2) Apoptosis was detected by TUNEL assay. Migration and tube formation ability was detected by Transwell chamber and Matrigel assays, respectively. The expression and activation of β-catenin was detected by Western blot. 3) Doppler flowmetry was used to detect the effect of FGF2 on blood flow recovery in ischemic hind limbs of mice. Results: 1) FGF-2 treatment reversed high glucose induced growth inhibition of EPCs. FGF-2 treatment also increased migration and tube formation ability of EPCs even in high glucose conditions. 2) Western blot analysis demonstrated that the percentage of activated β-catenin/total β-catenin in the high glucose group were significantly lower than that in the control group, while FGF-2 treatment reversed high glucose induced β-catenin inhibition. 3) In vivo experiments demonstrated that the blood flow recovery in ischemic hind limbs of mice was significantly improved after FGF-2 treatment. Conclusion: Exogenous FGF-2 could play a role in the functional repair of damaged EPC exposed to high glucose conditions, via the activation of the Wnt/β-catenin signaling pathway. |
format | Online Article Text |
id | pubmed-8586578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Shanghai Journal of Interventional Radiology Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-85865782021-11-19 Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions Li, Yang Li, Xue Han, Shilong Lian, Weishuai Cheng, Jie Xie, Xiaoyun Li, Maoquan J Interv Med Basic Research Purpose: To investigate the effects of exogenous basic fibroblast growth factor -2 (FGF-2) on the biological activity of endothelial progenitor cells (EPCs) exposed to high glucose conditions. Materials and Methods: 1) Bone marrow EPCs from C57BL/6 mice were isolated and cultured in vitro. EPC purity was identified by flow cytometry and immunofluorescence staining. 2) Apoptosis was detected by TUNEL assay. Migration and tube formation ability was detected by Transwell chamber and Matrigel assays, respectively. The expression and activation of β-catenin was detected by Western blot. 3) Doppler flowmetry was used to detect the effect of FGF2 on blood flow recovery in ischemic hind limbs of mice. Results: 1) FGF-2 treatment reversed high glucose induced growth inhibition of EPCs. FGF-2 treatment also increased migration and tube formation ability of EPCs even in high glucose conditions. 2) Western blot analysis demonstrated that the percentage of activated β-catenin/total β-catenin in the high glucose group were significantly lower than that in the control group, while FGF-2 treatment reversed high glucose induced β-catenin inhibition. 3) In vivo experiments demonstrated that the blood flow recovery in ischemic hind limbs of mice was significantly improved after FGF-2 treatment. Conclusion: Exogenous FGF-2 could play a role in the functional repair of damaged EPC exposed to high glucose conditions, via the activation of the Wnt/β-catenin signaling pathway. Shanghai Journal of Interventional Radiology Press 2019-04-30 /pmc/articles/PMC8586578/ /pubmed/34805825 http://dx.doi.org/10.19779/j.cnki.2096-3602.2018.01.04 Text en © 2018 Shanghai Journal of Interventional Medicine Press. Production and hosting by Elsevier B.V. on behalf of KeAi. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Basic Research Li, Yang Li, Xue Han, Shilong Lian, Weishuai Cheng, Jie Xie, Xiaoyun Li, Maoquan Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions |
title | Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions |
title_full | Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions |
title_fullStr | Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions |
title_full_unstemmed | Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions |
title_short | Exogenous FGF-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions |
title_sort | exogenous fgf-2 improves biological activity of endothelial progenitor cells exposed to high glucose conditions |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586578/ https://www.ncbi.nlm.nih.gov/pubmed/34805825 http://dx.doi.org/10.19779/j.cnki.2096-3602.2018.01.04 |
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