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High-Resolution Mass Spectrometry and Chemometrics for the Detailed Characterization of Short Endogenous Peptides in Milk By-Products

The process of cheese-making has long been part of human food culture and nowadays dairy represents a large sector of the food industry. Being the main byproduct of cheese-making, the revalorization of milk whey is nowadays one of the primary goals in alignment with the principles of the circular ec...

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Autores principales: Montone, Carmela Maria, Aita, Sara Elsa, Cavaliere, Chiara, Cerrato, Andrea, Laganà, Aldo, Piovesana, Susy, Capriotti, Anna Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587072/
https://www.ncbi.nlm.nih.gov/pubmed/34770881
http://dx.doi.org/10.3390/molecules26216472
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author Montone, Carmela Maria
Aita, Sara Elsa
Cavaliere, Chiara
Cerrato, Andrea
Laganà, Aldo
Piovesana, Susy
Capriotti, Anna Laura
author_facet Montone, Carmela Maria
Aita, Sara Elsa
Cavaliere, Chiara
Cerrato, Andrea
Laganà, Aldo
Piovesana, Susy
Capriotti, Anna Laura
author_sort Montone, Carmela Maria
collection PubMed
description The process of cheese-making has long been part of human food culture and nowadays dairy represents a large sector of the food industry. Being the main byproduct of cheese-making, the revalorization of milk whey is nowadays one of the primary goals in alignment with the principles of the circular economy. In the present paper, a deep and detailed investigation of short endogenous peptides in milk and its byproducts (whole whey, skimmed whey, and whey permeate) was carried out by high-resolution mass spectrometry, with a dedicated suspect screening data acquisition and data analysis approach. A total of 79 short peptides was tentatively identified, including several sequences already known for their exerted biological activities. An unsupervised chemometric approach was then employed for highlighting the differences in the short peptide content among the four sets of samples. Whole and skimmed whey showed not merely a higher content of short bioactive peptides compared to whole milk, but also a peculiar composition of peptides that are likely generated during the process of cheese-making. The results clearly demonstrate that whey represents a valuable source of bioactive compounds and that the set-up of processes of revalorization of milk byproducts is a promising path in the obtention of high revenue-generating products from dairy industrial waste.
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spelling pubmed-85870722021-11-13 High-Resolution Mass Spectrometry and Chemometrics for the Detailed Characterization of Short Endogenous Peptides in Milk By-Products Montone, Carmela Maria Aita, Sara Elsa Cavaliere, Chiara Cerrato, Andrea Laganà, Aldo Piovesana, Susy Capriotti, Anna Laura Molecules Article The process of cheese-making has long been part of human food culture and nowadays dairy represents a large sector of the food industry. Being the main byproduct of cheese-making, the revalorization of milk whey is nowadays one of the primary goals in alignment with the principles of the circular economy. In the present paper, a deep and detailed investigation of short endogenous peptides in milk and its byproducts (whole whey, skimmed whey, and whey permeate) was carried out by high-resolution mass spectrometry, with a dedicated suspect screening data acquisition and data analysis approach. A total of 79 short peptides was tentatively identified, including several sequences already known for their exerted biological activities. An unsupervised chemometric approach was then employed for highlighting the differences in the short peptide content among the four sets of samples. Whole and skimmed whey showed not merely a higher content of short bioactive peptides compared to whole milk, but also a peculiar composition of peptides that are likely generated during the process of cheese-making. The results clearly demonstrate that whey represents a valuable source of bioactive compounds and that the set-up of processes of revalorization of milk byproducts is a promising path in the obtention of high revenue-generating products from dairy industrial waste. MDPI 2021-10-27 /pmc/articles/PMC8587072/ /pubmed/34770881 http://dx.doi.org/10.3390/molecules26216472 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Montone, Carmela Maria
Aita, Sara Elsa
Cavaliere, Chiara
Cerrato, Andrea
Laganà, Aldo
Piovesana, Susy
Capriotti, Anna Laura
High-Resolution Mass Spectrometry and Chemometrics for the Detailed Characterization of Short Endogenous Peptides in Milk By-Products
title High-Resolution Mass Spectrometry and Chemometrics for the Detailed Characterization of Short Endogenous Peptides in Milk By-Products
title_full High-Resolution Mass Spectrometry and Chemometrics for the Detailed Characterization of Short Endogenous Peptides in Milk By-Products
title_fullStr High-Resolution Mass Spectrometry and Chemometrics for the Detailed Characterization of Short Endogenous Peptides in Milk By-Products
title_full_unstemmed High-Resolution Mass Spectrometry and Chemometrics for the Detailed Characterization of Short Endogenous Peptides in Milk By-Products
title_short High-Resolution Mass Spectrometry and Chemometrics for the Detailed Characterization of Short Endogenous Peptides in Milk By-Products
title_sort high-resolution mass spectrometry and chemometrics for the detailed characterization of short endogenous peptides in milk by-products
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587072/
https://www.ncbi.nlm.nih.gov/pubmed/34770881
http://dx.doi.org/10.3390/molecules26216472
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