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Novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry

BACKGROUND: Red ginseng polysaccharides (RGPs) have been acknowledged for their outstanding immunomodulation and anti-tumor activities. However, their studies are still limited by the complexity of their structural features, the absence of purification and enrichment methods, and the rarity of the a...

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Autores principales: Jin, Ye Rin, Oh, Myung Jin, Yuk, Heung Joo, An, Hyun Joo, Kim, Dong Seon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587486/
https://www.ncbi.nlm.nih.gov/pubmed/34803423
http://dx.doi.org/10.1016/j.jgr.2021.02.005
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author Jin, Ye Rin
Oh, Myung Jin
Yuk, Heung Joo
An, Hyun Joo
Kim, Dong Seon
author_facet Jin, Ye Rin
Oh, Myung Jin
Yuk, Heung Joo
An, Hyun Joo
Kim, Dong Seon
author_sort Jin, Ye Rin
collection PubMed
description BACKGROUND: Red ginseng polysaccharides (RGPs) have been acknowledged for their outstanding immunomodulation and anti-tumor activities. However, their studies are still limited by the complexity of their structural features, the absence of purification and enrichment methods, and the rarity of the analytical instruments that apply to the analysis of such macromolecules. Thus, this study is an attempt to establish a new mass spectrometry (MS)-based analysis procedure for RGPs. METHODS: Saponin pre-excluded powder of RG (RG-SPEP, 10 mg) was treated with 200 μL of distilled water and centrifuged for 5 h at 1000 rpm and 85 °C. Ethanol-based precipitation and centrifugation were applied to obtain RGPs from the heated extracts. Further, endo-carbohydrase treatments were performed to produce specific saccharide fragments. Solid-phase extraction (SPE) processes were implemented to purify and enrich the enzyme-treated RGPs, while matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) MS was employed for the partial structural analysis of the obtained RGPs. RESULTS: Utilizing cellulase, porous graphitized carbon (PGC), hydrophilic interaction chromatography (HILIC), and MALDI-TOF/TOF MS, the neutral and acidic RGPs were qualitatively analyzed. Hex(n) and Hex(n)−18 (cellulose analogs) were determined to be novel neutral RGPs. Additionally, the [Unknown + Hex(n)] species were also determined as new acidic RGPs. Furthermore, HexA(n) (H) was determined as another form of the acidic RGPs. CONCLUSION: Compared to the previous methods of analysis, these unprecedented applications of HILIC-SPE and MALDI-TOF/TOF MS to analyze RGPs proved to be fairly effective for fractionating and detecting neutral and acidic components. This new procedure exhibits great potential as a specific tool for searching and determining various polysaccharides in many herbal medicines.
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spelling pubmed-85874862021-11-19 Novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry Jin, Ye Rin Oh, Myung Jin Yuk, Heung Joo An, Hyun Joo Kim, Dong Seon J Ginseng Res Chemistry BACKGROUND: Red ginseng polysaccharides (RGPs) have been acknowledged for their outstanding immunomodulation and anti-tumor activities. However, their studies are still limited by the complexity of their structural features, the absence of purification and enrichment methods, and the rarity of the analytical instruments that apply to the analysis of such macromolecules. Thus, this study is an attempt to establish a new mass spectrometry (MS)-based analysis procedure for RGPs. METHODS: Saponin pre-excluded powder of RG (RG-SPEP, 10 mg) was treated with 200 μL of distilled water and centrifuged for 5 h at 1000 rpm and 85 °C. Ethanol-based precipitation and centrifugation were applied to obtain RGPs from the heated extracts. Further, endo-carbohydrase treatments were performed to produce specific saccharide fragments. Solid-phase extraction (SPE) processes were implemented to purify and enrich the enzyme-treated RGPs, while matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) MS was employed for the partial structural analysis of the obtained RGPs. RESULTS: Utilizing cellulase, porous graphitized carbon (PGC), hydrophilic interaction chromatography (HILIC), and MALDI-TOF/TOF MS, the neutral and acidic RGPs were qualitatively analyzed. Hex(n) and Hex(n)−18 (cellulose analogs) were determined to be novel neutral RGPs. Additionally, the [Unknown + Hex(n)] species were also determined as new acidic RGPs. Furthermore, HexA(n) (H) was determined as another form of the acidic RGPs. CONCLUSION: Compared to the previous methods of analysis, these unprecedented applications of HILIC-SPE and MALDI-TOF/TOF MS to analyze RGPs proved to be fairly effective for fractionating and detecting neutral and acidic components. This new procedure exhibits great potential as a specific tool for searching and determining various polysaccharides in many herbal medicines. Elsevier 2021-09 2021-02-21 /pmc/articles/PMC8587486/ /pubmed/34803423 http://dx.doi.org/10.1016/j.jgr.2021.02.005 Text en © 2021 The Korean Society of Ginseng. Publishing services by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Chemistry
Jin, Ye Rin
Oh, Myung Jin
Yuk, Heung Joo
An, Hyun Joo
Kim, Dong Seon
Novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry
title Novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry
title_full Novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry
title_fullStr Novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry
title_full_unstemmed Novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry
title_short Novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry
title_sort novel analysis procedure for red ginseng polysaccharides by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587486/
https://www.ncbi.nlm.nih.gov/pubmed/34803423
http://dx.doi.org/10.1016/j.jgr.2021.02.005
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