Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy

[Image: see text] We propose a series of fluorescent dyes with hydrophilic carbamate caging groups that undergo rapid photoactivation under UV (≤400 nm) irradiation but do not undergo spurious two-photon activation with high-intensity (visible or infrared) light of about twice the wavelength. The ca...

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Autores principales: Butkevich, Alexey N., Weber, Michael, Cereceda Delgado, Angel R., Ostersehlt, Lynn M., D’Este, Elisa, Hell, Stefan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587603/
https://www.ncbi.nlm.nih.gov/pubmed/34714070
http://dx.doi.org/10.1021/jacs.1c09999
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author Butkevich, Alexey N.
Weber, Michael
Cereceda Delgado, Angel R.
Ostersehlt, Lynn M.
D’Este, Elisa
Hell, Stefan W.
author_facet Butkevich, Alexey N.
Weber, Michael
Cereceda Delgado, Angel R.
Ostersehlt, Lynn M.
D’Este, Elisa
Hell, Stefan W.
author_sort Butkevich, Alexey N.
collection PubMed
description [Image: see text] We propose a series of fluorescent dyes with hydrophilic carbamate caging groups that undergo rapid photoactivation under UV (≤400 nm) irradiation but do not undergo spurious two-photon activation with high-intensity (visible or infrared) light of about twice the wavelength. The caged fluorescent dyes and labels derived therefrom display high water solubility and convert upon photoactivation into validated super-resolution and live-cell-compatible fluorophores. In combination with popular fluorescent markers, multiple (up to six)-color images can be obtained with stimulated emission depletion nanoscopy. Moreover, individual fluorophores can be localized with precision <3 nm (standard deviation) using MINSTED and MINFLUX techniques.
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spelling pubmed-85876032021-11-12 Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy Butkevich, Alexey N. Weber, Michael Cereceda Delgado, Angel R. Ostersehlt, Lynn M. D’Este, Elisa Hell, Stefan W. J Am Chem Soc [Image: see text] We propose a series of fluorescent dyes with hydrophilic carbamate caging groups that undergo rapid photoactivation under UV (≤400 nm) irradiation but do not undergo spurious two-photon activation with high-intensity (visible or infrared) light of about twice the wavelength. The caged fluorescent dyes and labels derived therefrom display high water solubility and convert upon photoactivation into validated super-resolution and live-cell-compatible fluorophores. In combination with popular fluorescent markers, multiple (up to six)-color images can be obtained with stimulated emission depletion nanoscopy. Moreover, individual fluorophores can be localized with precision <3 nm (standard deviation) using MINSTED and MINFLUX techniques. American Chemical Society 2021-10-29 2021-11-10 /pmc/articles/PMC8587603/ /pubmed/34714070 http://dx.doi.org/10.1021/jacs.1c09999 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Butkevich, Alexey N.
Weber, Michael
Cereceda Delgado, Angel R.
Ostersehlt, Lynn M.
D’Este, Elisa
Hell, Stefan W.
Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy
title Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy
title_full Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy
title_fullStr Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy
title_full_unstemmed Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy
title_short Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy
title_sort photoactivatable fluorescent dyes with hydrophilic caging groups and their use in multicolor nanoscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587603/
https://www.ncbi.nlm.nih.gov/pubmed/34714070
http://dx.doi.org/10.1021/jacs.1c09999
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