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Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro

The antiangiogenic activity of the ethanol extract of propolis collected from different regions in western Algeria was investigated using in vitro human umbilical vein endothelial cells (HUVECs). The ethanol extract with the strongest activity, i.e., Algerian propolis 1 (EEPA1), inhibited the format...

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Autores principales: Hosoya, Takahiro, Tsuchiya, Ikumi, Ohta, Toshiro, Benhanifia, Mokhtar, Kumazawa, Shigenori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587774/
https://www.ncbi.nlm.nih.gov/pubmed/34770923
http://dx.doi.org/10.3390/molecules26216510
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author Hosoya, Takahiro
Tsuchiya, Ikumi
Ohta, Toshiro
Benhanifia, Mokhtar
Kumazawa, Shigenori
author_facet Hosoya, Takahiro
Tsuchiya, Ikumi
Ohta, Toshiro
Benhanifia, Mokhtar
Kumazawa, Shigenori
author_sort Hosoya, Takahiro
collection PubMed
description The antiangiogenic activity of the ethanol extract of propolis collected from different regions in western Algeria was investigated using in vitro human umbilical vein endothelial cells (HUVECs). The ethanol extract with the strongest activity, i.e., Algerian propolis 1 (EEPA1), inhibited the formation of capillary networks in a dose-dependent manner (6.25–50 μg/mL) within 12 h and induced cell fragmentation of HUVECs at 50 μg/mL after treatment for 24 h. To identify the active compounds in EEAP1, a high-performance liquid chromatography (HPLC) analysis was performed, revealing that EEAP1 contains two major compounds. Both compounds were isolated by repeated column chromatography and identified as ω-hydroxyferulenol (1) and ferulenol (2), which have a coumarin structure conjugated with a farnesyl group according to NMR, high-resolution electrospray ionization mass spectroscopy, and chemical modification. Compounds 1 and 2 inhibited the tube-forming activity of HUVECs, especially 2, which exhibited a stronger antiangiogenic effect even at a low concentration of 3.31 μg/mL. Moreover, 2 suppressed the elongation and induced cell fragmentation at the same dose. The molecular changes in tube-forming HUVECs induced by 2 were found to be related to the activation of the caspase signals. To confirm the plant origin of propolis, an HPLC comparative analysis of the ethanol extracts of some plants near beekeeping areas and that of Algerian propolis (EEAP1) was performed, and similar chromatographic patterns were observed. This result suggests that the plant origin of this Algerian propolis is the resin of Ferula communis.
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spelling pubmed-85877742021-11-13 Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro Hosoya, Takahiro Tsuchiya, Ikumi Ohta, Toshiro Benhanifia, Mokhtar Kumazawa, Shigenori Molecules Article The antiangiogenic activity of the ethanol extract of propolis collected from different regions in western Algeria was investigated using in vitro human umbilical vein endothelial cells (HUVECs). The ethanol extract with the strongest activity, i.e., Algerian propolis 1 (EEPA1), inhibited the formation of capillary networks in a dose-dependent manner (6.25–50 μg/mL) within 12 h and induced cell fragmentation of HUVECs at 50 μg/mL after treatment for 24 h. To identify the active compounds in EEAP1, a high-performance liquid chromatography (HPLC) analysis was performed, revealing that EEAP1 contains two major compounds. Both compounds were isolated by repeated column chromatography and identified as ω-hydroxyferulenol (1) and ferulenol (2), which have a coumarin structure conjugated with a farnesyl group according to NMR, high-resolution electrospray ionization mass spectroscopy, and chemical modification. Compounds 1 and 2 inhibited the tube-forming activity of HUVECs, especially 2, which exhibited a stronger antiangiogenic effect even at a low concentration of 3.31 μg/mL. Moreover, 2 suppressed the elongation and induced cell fragmentation at the same dose. The molecular changes in tube-forming HUVECs induced by 2 were found to be related to the activation of the caspase signals. To confirm the plant origin of propolis, an HPLC comparative analysis of the ethanol extracts of some plants near beekeeping areas and that of Algerian propolis (EEAP1) was performed, and similar chromatographic patterns were observed. This result suggests that the plant origin of this Algerian propolis is the resin of Ferula communis. MDPI 2021-10-28 /pmc/articles/PMC8587774/ /pubmed/34770923 http://dx.doi.org/10.3390/molecules26216510 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hosoya, Takahiro
Tsuchiya, Ikumi
Ohta, Toshiro
Benhanifia, Mokhtar
Kumazawa, Shigenori
Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_full Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_fullStr Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_full_unstemmed Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_short Composition of Algerian Propolis, Plant Origin, and Its Antiangiogenic Activity In Vitro
title_sort composition of algerian propolis, plant origin, and its antiangiogenic activity in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587774/
https://www.ncbi.nlm.nih.gov/pubmed/34770923
http://dx.doi.org/10.3390/molecules26216510
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