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The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover

Bio-based treatment technologies are gaining great interest worldwide, and significant efforts are being afforded to develop technology for the use of lignocellulosic biomass. The potential of corn stover (CS) as a feedstock for bioethanol production was investigated by creating an optimal pretreatm...

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Autores principales: Hamdy, Ali, Abd Elhafez, Sara, Hamad, Hesham, Ali, Rehab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587947/
https://www.ncbi.nlm.nih.gov/pubmed/34771319
http://dx.doi.org/10.3390/polym13213762
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author Hamdy, Ali
Abd Elhafez, Sara
Hamad, Hesham
Ali, Rehab
author_facet Hamdy, Ali
Abd Elhafez, Sara
Hamad, Hesham
Ali, Rehab
author_sort Hamdy, Ali
collection PubMed
description Bio-based treatment technologies are gaining great interest worldwide, and significant efforts are being afforded to develop technology for the use of lignocellulosic biomass. The potential of corn stover (CS) as a feedstock for bioethanol production was investigated by creating an optimal pretreatment condition to maximize glucose production. The current study undertook the impact of novel physico-chemical pretreatment methods of CS, i.e., autoclave-assisted oxalate (CSOA) and ultrasound-assisted oxalate (CSOU), on the chemical composition of CS and subsequent saccharification and fermentation for bioethanol production. The delignification was monitored by physicochemical characterizations such as SEM, XRD, FTIR, CHNs, and TGA. The results evidenced that delignification and enzymatic saccharification of the CS pretreated by CSOA was higher than CSOU. The optimum enzymatic saccharification operating conditions were 1:30 g solid substrate/mL sodium acetate buffer at 50 °C, shaking speed 100 rpm, and 0.4 g enzyme dosage. This condition was applied to produce glucose from CS, followed by bioethanol production by S. cerevisiae using an anaerobic fermentation process after 72 h. S. cerevisiae showed high conversion efficiency by producing a 360 mg/dL bioethanol yield, which is considered 94.11% of the theoretical ethanol yield. Furthermore, this research provides a potential path for waste material beneficiation, such as through utilizing CS.
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spelling pubmed-85879472021-11-13 The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover Hamdy, Ali Abd Elhafez, Sara Hamad, Hesham Ali, Rehab Polymers (Basel) Article Bio-based treatment technologies are gaining great interest worldwide, and significant efforts are being afforded to develop technology for the use of lignocellulosic biomass. The potential of corn stover (CS) as a feedstock for bioethanol production was investigated by creating an optimal pretreatment condition to maximize glucose production. The current study undertook the impact of novel physico-chemical pretreatment methods of CS, i.e., autoclave-assisted oxalate (CSOA) and ultrasound-assisted oxalate (CSOU), on the chemical composition of CS and subsequent saccharification and fermentation for bioethanol production. The delignification was monitored by physicochemical characterizations such as SEM, XRD, FTIR, CHNs, and TGA. The results evidenced that delignification and enzymatic saccharification of the CS pretreated by CSOA was higher than CSOU. The optimum enzymatic saccharification operating conditions were 1:30 g solid substrate/mL sodium acetate buffer at 50 °C, shaking speed 100 rpm, and 0.4 g enzyme dosage. This condition was applied to produce glucose from CS, followed by bioethanol production by S. cerevisiae using an anaerobic fermentation process after 72 h. S. cerevisiae showed high conversion efficiency by producing a 360 mg/dL bioethanol yield, which is considered 94.11% of the theoretical ethanol yield. Furthermore, this research provides a potential path for waste material beneficiation, such as through utilizing CS. MDPI 2021-10-30 /pmc/articles/PMC8587947/ /pubmed/34771319 http://dx.doi.org/10.3390/polym13213762 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hamdy, Ali
Abd Elhafez, Sara
Hamad, Hesham
Ali, Rehab
The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_full The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_fullStr The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_full_unstemmed The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_short The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover
title_sort interplay of autoclaving with oxalate as pretreatment technique in the view of bioethanol production based on corn stover
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8587947/
https://www.ncbi.nlm.nih.gov/pubmed/34771319
http://dx.doi.org/10.3390/polym13213762
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