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Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation

Several significant advancements in the field of bone regenerative medicine have been made in recent years. However, therapeutic options, such as bone grafts, have several drawbacks. There is a need to develop an adequate bone substitute. As a result, significant bone defects/injuries pose a severe...

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Autores principales: Khan, Muhammad Umar Aslam, Razak, Saiful Izwan Abd, Ansari, Mohamed Nainar Mohamed, Zulkifli, Razauden Mohamed, Ahmad Zawawi, Nurliyana, Arshad, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588120/
https://www.ncbi.nlm.nih.gov/pubmed/34771168
http://dx.doi.org/10.3390/polym13213611
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author Khan, Muhammad Umar Aslam
Razak, Saiful Izwan Abd
Ansari, Mohamed Nainar Mohamed
Zulkifli, Razauden Mohamed
Ahmad Zawawi, Nurliyana
Arshad, Muhammad
author_facet Khan, Muhammad Umar Aslam
Razak, Saiful Izwan Abd
Ansari, Mohamed Nainar Mohamed
Zulkifli, Razauden Mohamed
Ahmad Zawawi, Nurliyana
Arshad, Muhammad
author_sort Khan, Muhammad Umar Aslam
collection PubMed
description Several significant advancements in the field of bone regenerative medicine have been made in recent years. However, therapeutic options, such as bone grafts, have several drawbacks. There is a need to develop an adequate bone substitute. As a result, significant bone defects/injuries pose a severe challenge for orthopaedic and reconstructive bone tissue. We synthesized polymeric composite material from arabinoxylan (ARX), β-glucan (BG), nano-hydroxyapatite (nHAp), graphene oxide (GO), acrylic acid (AAc) through free radical polymerization and porous scaffold fabricated using the freeze-drying technique. These fabricated porous scaffolds were then coated with chitosan solution to enhance their biological activities. The complex structure of BG, nHAp, GO was studied through various characterization and biological assays. The structural, morphological, wetting and mechanical analyses were determined using FT-IR, XRD, XPS, SEM/EXD, water contact angle and UTM. The swelling (aqueous and PBS media) and degradation (PBS media) observed their behavior in contact with body fluid. The biological activities were conducted against mouse pre-osteoblast cell lines. The result found that BGH3 has desirable morphological, structural with optimum swelling, degradation, and mechanical behavior. It was also found to be cytocompatible against MC3T3-E1 cell lines. The obtained results confirmed that the fabricated polymeric scaffolds would be a potential bone substitute to regenerate defective bone with different loading bearing applications for bone tissue engineering.
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spelling pubmed-85881202021-11-13 Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation Khan, Muhammad Umar Aslam Razak, Saiful Izwan Abd Ansari, Mohamed Nainar Mohamed Zulkifli, Razauden Mohamed Ahmad Zawawi, Nurliyana Arshad, Muhammad Polymers (Basel) Article Several significant advancements in the field of bone regenerative medicine have been made in recent years. However, therapeutic options, such as bone grafts, have several drawbacks. There is a need to develop an adequate bone substitute. As a result, significant bone defects/injuries pose a severe challenge for orthopaedic and reconstructive bone tissue. We synthesized polymeric composite material from arabinoxylan (ARX), β-glucan (BG), nano-hydroxyapatite (nHAp), graphene oxide (GO), acrylic acid (AAc) through free radical polymerization and porous scaffold fabricated using the freeze-drying technique. These fabricated porous scaffolds were then coated with chitosan solution to enhance their biological activities. The complex structure of BG, nHAp, GO was studied through various characterization and biological assays. The structural, morphological, wetting and mechanical analyses were determined using FT-IR, XRD, XPS, SEM/EXD, water contact angle and UTM. The swelling (aqueous and PBS media) and degradation (PBS media) observed their behavior in contact with body fluid. The biological activities were conducted against mouse pre-osteoblast cell lines. The result found that BGH3 has desirable morphological, structural with optimum swelling, degradation, and mechanical behavior. It was also found to be cytocompatible against MC3T3-E1 cell lines. The obtained results confirmed that the fabricated polymeric scaffolds would be a potential bone substitute to regenerate defective bone with different loading bearing applications for bone tissue engineering. MDPI 2021-10-20 /pmc/articles/PMC8588120/ /pubmed/34771168 http://dx.doi.org/10.3390/polym13213611 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Khan, Muhammad Umar Aslam
Razak, Saiful Izwan Abd
Ansari, Mohamed Nainar Mohamed
Zulkifli, Razauden Mohamed
Ahmad Zawawi, Nurliyana
Arshad, Muhammad
Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_full Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_fullStr Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_full_unstemmed Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_short Development of Biodegradable Bio-Based Composite for Bone Tissue Engineering: Synthesis, Characterization and In Vitro Biocompatible Evaluation
title_sort development of biodegradable bio-based composite for bone tissue engineering: synthesis, characterization and in vitro biocompatible evaluation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588120/
https://www.ncbi.nlm.nih.gov/pubmed/34771168
http://dx.doi.org/10.3390/polym13213611
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