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Isoniazid—Loaded Albumin Nanoparticles: Taguchi Optimization Method

Tuberculosis is one of the dangerous infectious diseases, killing over a million people worldwide each year. The search for new dosage forms for the treatment of drug-resistant tuberculosis is an actual task. Biocompatible polymer nanoparticles, in particular bovine serum albumin (BSA), are promisin...

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Autores principales: Tazhbayev, Yerkeblan, Galiyeva, Aldana, Zhumagaliyeva, Tolkyn, Burkeyev, Meiram, Karimova, Bakhytgul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588201/
https://www.ncbi.nlm.nih.gov/pubmed/34771365
http://dx.doi.org/10.3390/polym13213808
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author Tazhbayev, Yerkeblan
Galiyeva, Aldana
Zhumagaliyeva, Tolkyn
Burkeyev, Meiram
Karimova, Bakhytgul
author_facet Tazhbayev, Yerkeblan
Galiyeva, Aldana
Zhumagaliyeva, Tolkyn
Burkeyev, Meiram
Karimova, Bakhytgul
author_sort Tazhbayev, Yerkeblan
collection PubMed
description Tuberculosis is one of the dangerous infectious diseases, killing over a million people worldwide each year. The search for new dosage forms for the treatment of drug-resistant tuberculosis is an actual task. Biocompatible polymer nanoparticles, in particular bovine serum albumin (BSA), are promising drug carriers. Nanoparticle (NP) parameters such as diameter, polydispersity, bioactive substance loading, and NP yield are very important when it comes to drug transport through the bloodstream. The most well-known and widely used first-line anti-tuberculosis drug, isoniazid (INH), is being used as a drug. BSA-INH NPs were obtained by an ethanol desolvation of an aqueous protein solution in the drug presence. The peculiarity of the method is that natural components, namely urea and cysteine, are used for the stabilization of BSA-INH NPs after desolvation. The characteristics of the obtained BSA-INH NPs are significantly affected by the concentration of protein, isoniazid, urea, and cysteine in the solution. The aim of the present study is to investigate the concentration effect of the system reacting components on the parameters of the NPs that are obtained. We have chosen the concentrations of four reacting components, i.e., BSA, isoniazid, urea, and cysteine, as controlling factors and applied the Taguchi method to analyze which concentration of each component has an important effect on BSA-INH NPs characteristics.
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spelling pubmed-85882012021-11-13 Isoniazid—Loaded Albumin Nanoparticles: Taguchi Optimization Method Tazhbayev, Yerkeblan Galiyeva, Aldana Zhumagaliyeva, Tolkyn Burkeyev, Meiram Karimova, Bakhytgul Polymers (Basel) Article Tuberculosis is one of the dangerous infectious diseases, killing over a million people worldwide each year. The search for new dosage forms for the treatment of drug-resistant tuberculosis is an actual task. Biocompatible polymer nanoparticles, in particular bovine serum albumin (BSA), are promising drug carriers. Nanoparticle (NP) parameters such as diameter, polydispersity, bioactive substance loading, and NP yield are very important when it comes to drug transport through the bloodstream. The most well-known and widely used first-line anti-tuberculosis drug, isoniazid (INH), is being used as a drug. BSA-INH NPs were obtained by an ethanol desolvation of an aqueous protein solution in the drug presence. The peculiarity of the method is that natural components, namely urea and cysteine, are used for the stabilization of BSA-INH NPs after desolvation. The characteristics of the obtained BSA-INH NPs are significantly affected by the concentration of protein, isoniazid, urea, and cysteine in the solution. The aim of the present study is to investigate the concentration effect of the system reacting components on the parameters of the NPs that are obtained. We have chosen the concentrations of four reacting components, i.e., BSA, isoniazid, urea, and cysteine, as controlling factors and applied the Taguchi method to analyze which concentration of each component has an important effect on BSA-INH NPs characteristics. MDPI 2021-11-04 /pmc/articles/PMC8588201/ /pubmed/34771365 http://dx.doi.org/10.3390/polym13213808 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tazhbayev, Yerkeblan
Galiyeva, Aldana
Zhumagaliyeva, Tolkyn
Burkeyev, Meiram
Karimova, Bakhytgul
Isoniazid—Loaded Albumin Nanoparticles: Taguchi Optimization Method
title Isoniazid—Loaded Albumin Nanoparticles: Taguchi Optimization Method
title_full Isoniazid—Loaded Albumin Nanoparticles: Taguchi Optimization Method
title_fullStr Isoniazid—Loaded Albumin Nanoparticles: Taguchi Optimization Method
title_full_unstemmed Isoniazid—Loaded Albumin Nanoparticles: Taguchi Optimization Method
title_short Isoniazid—Loaded Albumin Nanoparticles: Taguchi Optimization Method
title_sort isoniazid—loaded albumin nanoparticles: taguchi optimization method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588201/
https://www.ncbi.nlm.nih.gov/pubmed/34771365
http://dx.doi.org/10.3390/polym13213808
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