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Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics

Pueraria lobata and its variety P. lobata var. thomsonii are both traditional Chinese medicines that have high nutritional and medical value; whereas another variety, P. lobata var. montana has low nutritional and medicinal value and can cause ecological disasters. The material basis of different nu...

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Autores principales: Shang, Xiaohong, Huang, Ding, Wang, Ying, Xiao, Liang, Ming, Ruhong, Zeng, Wendan, Cao, Sheng, Lu, Liuying, Wu, Zhengdan, Yan, Huabing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588241/
https://www.ncbi.nlm.nih.gov/pubmed/34770994
http://dx.doi.org/10.3390/molecules26216587
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author Shang, Xiaohong
Huang, Ding
Wang, Ying
Xiao, Liang
Ming, Ruhong
Zeng, Wendan
Cao, Sheng
Lu, Liuying
Wu, Zhengdan
Yan, Huabing
author_facet Shang, Xiaohong
Huang, Ding
Wang, Ying
Xiao, Liang
Ming, Ruhong
Zeng, Wendan
Cao, Sheng
Lu, Liuying
Wu, Zhengdan
Yan, Huabing
author_sort Shang, Xiaohong
collection PubMed
description Pueraria lobata and its variety P. lobata var. thomsonii are both traditional Chinese medicines that have high nutritional and medical value; whereas another variety, P. lobata var. montana has low nutritional and medicinal value and can cause ecological disasters. The material basis of different nutritional and medicinal values, which are caused by metabolite differences among these varieties, remains to be further clarified. Here, we performed ultra performance liquid chromatography-tandem mass spectrometry based widely targeted metabolome analysis on Pueraria lobata, P. lobata var. thomsonii, and P. lobata var. montana. Among them, a total of 614 metabolites were identified, and distinguished from each other using orthogonal partial least squares discriminant analysis. Our results suggest that the nutritional differences between P. lobata and its varieties can be explained by variations in the abundance of amino acids, nucleotides, saccharides, and lipids; differences in flavonoids, isoflavones, phenolic acids, organic acids, and coumarins contents caused the differences in the medicinal quality of P. lobata and its varieties. Additionally, the key metabolites responsible for the classification of the three Pueraria varieties were identified. This study provides new insights into the underlying metabolic causes of nutritional and medicinal variation in P. lobata and its varieties.
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spelling pubmed-85882412021-11-13 Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics Shang, Xiaohong Huang, Ding Wang, Ying Xiao, Liang Ming, Ruhong Zeng, Wendan Cao, Sheng Lu, Liuying Wu, Zhengdan Yan, Huabing Molecules Article Pueraria lobata and its variety P. lobata var. thomsonii are both traditional Chinese medicines that have high nutritional and medical value; whereas another variety, P. lobata var. montana has low nutritional and medicinal value and can cause ecological disasters. The material basis of different nutritional and medicinal values, which are caused by metabolite differences among these varieties, remains to be further clarified. Here, we performed ultra performance liquid chromatography-tandem mass spectrometry based widely targeted metabolome analysis on Pueraria lobata, P. lobata var. thomsonii, and P. lobata var. montana. Among them, a total of 614 metabolites were identified, and distinguished from each other using orthogonal partial least squares discriminant analysis. Our results suggest that the nutritional differences between P. lobata and its varieties can be explained by variations in the abundance of amino acids, nucleotides, saccharides, and lipids; differences in flavonoids, isoflavones, phenolic acids, organic acids, and coumarins contents caused the differences in the medicinal quality of P. lobata and its varieties. Additionally, the key metabolites responsible for the classification of the three Pueraria varieties were identified. This study provides new insights into the underlying metabolic causes of nutritional and medicinal variation in P. lobata and its varieties. MDPI 2021-10-30 /pmc/articles/PMC8588241/ /pubmed/34770994 http://dx.doi.org/10.3390/molecules26216587 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shang, Xiaohong
Huang, Ding
Wang, Ying
Xiao, Liang
Ming, Ruhong
Zeng, Wendan
Cao, Sheng
Lu, Liuying
Wu, Zhengdan
Yan, Huabing
Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_full Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_fullStr Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_full_unstemmed Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_short Identification of Nutritional Ingredients and Medicinal Components of Pueraria lobata and Its Varieties Using UPLC-MS/MS-Based Metabolomics
title_sort identification of nutritional ingredients and medicinal components of pueraria lobata and its varieties using uplc-ms/ms-based metabolomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588241/
https://www.ncbi.nlm.nih.gov/pubmed/34770994
http://dx.doi.org/10.3390/molecules26216587
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