MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression

BACKGROUND: So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). METHODS: MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods....

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Detalles Bibliográficos
Autores principales: Hu, Jiangwei, Xiang, Xinqin, Guan, Wei, Lou, Weihua, He, Junming, Chen, Jian, Fu, Yin, Lou, Guoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588708/
https://www.ncbi.nlm.nih.gov/pubmed/34772428
http://dx.doi.org/10.1186/s13019-021-01698-2
Descripción
Sumario:BACKGROUND: So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). METHODS: MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. RESULTS: Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. CONCLUSION: By targeting CDCA4, miR-497-5p restrained development of LUSC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13019-021-01698-2.

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