MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression

BACKGROUND: So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). METHODS: MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods....

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Autores principales: Hu, Jiangwei, Xiang, Xinqin, Guan, Wei, Lou, Weihua, He, Junming, Chen, Jian, Fu, Yin, Lou, Guoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588708/
https://www.ncbi.nlm.nih.gov/pubmed/34772428
http://dx.doi.org/10.1186/s13019-021-01698-2
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author Hu, Jiangwei
Xiang, Xinqin
Guan, Wei
Lou, Weihua
He, Junming
Chen, Jian
Fu, Yin
Lou, Guoliang
author_facet Hu, Jiangwei
Xiang, Xinqin
Guan, Wei
Lou, Weihua
He, Junming
Chen, Jian
Fu, Yin
Lou, Guoliang
author_sort Hu, Jiangwei
collection PubMed
description BACKGROUND: So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). METHODS: MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. RESULTS: Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. CONCLUSION: By targeting CDCA4, miR-497-5p restrained development of LUSC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13019-021-01698-2.
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spelling pubmed-85887082021-11-15 MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression Hu, Jiangwei Xiang, Xinqin Guan, Wei Lou, Weihua He, Junming Chen, Jian Fu, Yin Lou, Guoliang J Cardiothorac Surg Research Article BACKGROUND: So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC). METHODS: MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis. RESULTS: Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4. CONCLUSION: By targeting CDCA4, miR-497-5p restrained development of LUSC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13019-021-01698-2. BioMed Central 2021-11-12 /pmc/articles/PMC8588708/ /pubmed/34772428 http://dx.doi.org/10.1186/s13019-021-01698-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Hu, Jiangwei
Xiang, Xinqin
Guan, Wei
Lou, Weihua
He, Junming
Chen, Jian
Fu, Yin
Lou, Guoliang
MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_full MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_fullStr MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_full_unstemmed MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_short MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
title_sort mir-497-5p down-regulates cdca4 to restrains lung squamous cell carcinoma progression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588708/
https://www.ncbi.nlm.nih.gov/pubmed/34772428
http://dx.doi.org/10.1186/s13019-021-01698-2
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