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Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim
Juglans mandshurica Maxim is a hermaphroditic plant belonging to the genus Juglans in the family Juglandaceae. The pollination period of female flowers is different from the loose powder period of male flowers on the same tree. In several trees, female flowers bloom first, whereas in others, male fl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588858/ https://www.ncbi.nlm.nih.gov/pubmed/34820167 http://dx.doi.org/10.7717/peerj.12328 |
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author | Qin, Baiting Lu, Xiujun Sun, Xiaomei Cui, Jianguo Deng, Jifeng Zhang, Lijie |
author_facet | Qin, Baiting Lu, Xiujun Sun, Xiaomei Cui, Jianguo Deng, Jifeng Zhang, Lijie |
author_sort | Qin, Baiting |
collection | PubMed |
description | Juglans mandshurica Maxim is a hermaphroditic plant belonging to the genus Juglans in the family Juglandaceae. The pollination period of female flowers is different from the loose powder period of male flowers on the same tree. In several trees, female flowers bloom first, whereas in others, male flowers bloom first. In this study, male and female flower buds of J. mandshurica at the physiological differentiation stage were used. Illumina-based transcriptome sequencing was performed, and the quality of the sequencing results was evaluated and analyzed. A total of 138,138 unigenes with an average length of 788 bp were obtained. There were 8,116 differentially expressed genes (DEGs); 2,840 genes were upregulated, and 5,276 genes were downregulated. The DEGs were classified by Gene Ontology and analyzed by Kyoto Encyclopedia of Genes and Genomes. The signal transduction factors involved in phytohormone synthesis were selected. The results displayed that ARF and SAUR were expressed differently in the auxin signaling pathway. Additionally, DELLA protein (a negative regulator of gibberellin), the cytokinin synthesis pathway, and A-ARR were downregulated. On April 2nd, the contents of IAA, GA, CTK, ETH and SA in male and female flower buds of two types of J. mandshurica were opposite, and there were obvious genes regulating gender differentiation. Overall, we found that the sex differentiation of J. mandshurica was related to various hormone signal transduction pathways, and hormone signal transduction plays a leading role in regulation. |
format | Online Article Text |
id | pubmed-8588858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85888582021-11-23 Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim Qin, Baiting Lu, Xiujun Sun, Xiaomei Cui, Jianguo Deng, Jifeng Zhang, Lijie PeerJ Agricultural Science Juglans mandshurica Maxim is a hermaphroditic plant belonging to the genus Juglans in the family Juglandaceae. The pollination period of female flowers is different from the loose powder period of male flowers on the same tree. In several trees, female flowers bloom first, whereas in others, male flowers bloom first. In this study, male and female flower buds of J. mandshurica at the physiological differentiation stage were used. Illumina-based transcriptome sequencing was performed, and the quality of the sequencing results was evaluated and analyzed. A total of 138,138 unigenes with an average length of 788 bp were obtained. There were 8,116 differentially expressed genes (DEGs); 2,840 genes were upregulated, and 5,276 genes were downregulated. The DEGs were classified by Gene Ontology and analyzed by Kyoto Encyclopedia of Genes and Genomes. The signal transduction factors involved in phytohormone synthesis were selected. The results displayed that ARF and SAUR were expressed differently in the auxin signaling pathway. Additionally, DELLA protein (a negative regulator of gibberellin), the cytokinin synthesis pathway, and A-ARR were downregulated. On April 2nd, the contents of IAA, GA, CTK, ETH and SA in male and female flower buds of two types of J. mandshurica were opposite, and there were obvious genes regulating gender differentiation. Overall, we found that the sex differentiation of J. mandshurica was related to various hormone signal transduction pathways, and hormone signal transduction plays a leading role in regulation. PeerJ Inc. 2021-11-09 /pmc/articles/PMC8588858/ /pubmed/34820167 http://dx.doi.org/10.7717/peerj.12328 Text en © 2021 Qin et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Agricultural Science Qin, Baiting Lu, Xiujun Sun, Xiaomei Cui, Jianguo Deng, Jifeng Zhang, Lijie Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim |
title | Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim |
title_full | Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim |
title_fullStr | Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim |
title_full_unstemmed | Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim |
title_short | Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim |
title_sort | transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in juglans mandshurica maxim |
topic | Agricultural Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8588858/ https://www.ncbi.nlm.nih.gov/pubmed/34820167 http://dx.doi.org/10.7717/peerj.12328 |
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