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Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO(4) by Microbial Strains Using Glycine max as a Model
The sustainable development of agriculture can be stimulated by the great market availability of bio-inputs, including phosphate-solubilizing microbial strains. However, these strains are currently selected using imprecise and questionable solubilization methodologies in solid or liquid media. We hy...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589188/ https://www.ncbi.nlm.nih.gov/pubmed/34777440 http://dx.doi.org/10.3389/fpls.2021.759463 |
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author | Reis, Mateus Neri Oliveira Bessa, Layara Alexandre de Jesus, Andressa Pereira Guimarães Silva, Fabiano Moreira, Marialva Alvarenga Vitorino, Luciana Cristina |
author_facet | Reis, Mateus Neri Oliveira Bessa, Layara Alexandre de Jesus, Andressa Pereira Guimarães Silva, Fabiano Moreira, Marialva Alvarenga Vitorino, Luciana Cristina |
author_sort | Reis, Mateus Neri Oliveira |
collection | PubMed |
description | The sustainable development of agriculture can be stimulated by the great market availability of bio-inputs, including phosphate-solubilizing microbial strains. However, these strains are currently selected using imprecise and questionable solubilization methodologies in solid or liquid media. We hypothesized that the hydroponic system could be a more efficient methodology for selecting phosphate-solubilizing strains as plant growth promoters. This methodology was tested using the plant Glycine max as a model. The growth-promoting potential of the strains was compared with that of the Biomaphos® commercial microbial mixture. The obtained calcium phosphate (CaHPO(4)) solubilization results using the hydroponic system were inconsistent with those observed in solid and liquid media. However, the tests in liquid medium demonstrated poor performances of Codinaeopsis sp. (328EF) and Hamigera insecticola (33EF) in reducing pH and solubilizing CaHPO(4), which corroborates with the effects of biotic stress observed in G. max plants inoculated with these strains. Nevertheless, the hydroponic system allowed the characterization of Paenibacillus alvei (PA12), which is also efficient in solubilization in a liquid medium. The bacterium Lysinibacillus fusiformis (PA26) was the most effective in CaHPO(4) solubilization owing to the higher phosphorus (P) absorption, growth promotion, and physiological performance observed in plants inoculated with this bacterium. The hydroponic method proved to be superior in selecting solubilizing strains, allowing the assessment of multiple patterns, such as nutritional level, growth, photosynthetic performance, and anatomical variation in plants, and even the detection of biotic stress responses to inoculation, obtaining strains with higher growth promotion potential than Biomaphos®. This study proposed a new approach to confirm the solubilizing activity of microorganisms previously selected in vitro and potentially intended for the bio-input market that are useful in P availability for important crops, such as soybeans. |
format | Online Article Text |
id | pubmed-8589188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85891882021-11-13 Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO(4) by Microbial Strains Using Glycine max as a Model Reis, Mateus Neri Oliveira Bessa, Layara Alexandre de Jesus, Andressa Pereira Guimarães Silva, Fabiano Moreira, Marialva Alvarenga Vitorino, Luciana Cristina Front Plant Sci Plant Science The sustainable development of agriculture can be stimulated by the great market availability of bio-inputs, including phosphate-solubilizing microbial strains. However, these strains are currently selected using imprecise and questionable solubilization methodologies in solid or liquid media. We hypothesized that the hydroponic system could be a more efficient methodology for selecting phosphate-solubilizing strains as plant growth promoters. This methodology was tested using the plant Glycine max as a model. The growth-promoting potential of the strains was compared with that of the Biomaphos® commercial microbial mixture. The obtained calcium phosphate (CaHPO(4)) solubilization results using the hydroponic system were inconsistent with those observed in solid and liquid media. However, the tests in liquid medium demonstrated poor performances of Codinaeopsis sp. (328EF) and Hamigera insecticola (33EF) in reducing pH and solubilizing CaHPO(4), which corroborates with the effects of biotic stress observed in G. max plants inoculated with these strains. Nevertheless, the hydroponic system allowed the characterization of Paenibacillus alvei (PA12), which is also efficient in solubilization in a liquid medium. The bacterium Lysinibacillus fusiformis (PA26) was the most effective in CaHPO(4) solubilization owing to the higher phosphorus (P) absorption, growth promotion, and physiological performance observed in plants inoculated with this bacterium. The hydroponic method proved to be superior in selecting solubilizing strains, allowing the assessment of multiple patterns, such as nutritional level, growth, photosynthetic performance, and anatomical variation in plants, and even the detection of biotic stress responses to inoculation, obtaining strains with higher growth promotion potential than Biomaphos®. This study proposed a new approach to confirm the solubilizing activity of microorganisms previously selected in vitro and potentially intended for the bio-input market that are useful in P availability for important crops, such as soybeans. Frontiers Media S.A. 2021-10-29 /pmc/articles/PMC8589188/ /pubmed/34777440 http://dx.doi.org/10.3389/fpls.2021.759463 Text en Copyright © 2021 Reis, Bessa, de Jesus, Guimarães Silva, Moreira and Vitorino. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Reis, Mateus Neri Oliveira Bessa, Layara Alexandre de Jesus, Andressa Pereira Guimarães Silva, Fabiano Moreira, Marialva Alvarenga Vitorino, Luciana Cristina Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO(4) by Microbial Strains Using Glycine max as a Model |
title | Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO(4) by Microbial Strains Using Glycine max as a Model |
title_full | Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO(4) by Microbial Strains Using Glycine max as a Model |
title_fullStr | Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO(4) by Microbial Strains Using Glycine max as a Model |
title_full_unstemmed | Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO(4) by Microbial Strains Using Glycine max as a Model |
title_short | Efficiency of the Hydroponic System as an Approach to Confirm the Solubilization of CaHPO(4) by Microbial Strains Using Glycine max as a Model |
title_sort | efficiency of the hydroponic system as an approach to confirm the solubilization of cahpo(4) by microbial strains using glycine max as a model |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589188/ https://www.ncbi.nlm.nih.gov/pubmed/34777440 http://dx.doi.org/10.3389/fpls.2021.759463 |
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