A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines

CRISPR prime-editors are emergent tools for genome editing and offer a versatile alternative approach to HDR-based genome engineering or DNA base-editors. However, sufficient prime-editor expression levels and availability of optimized transfection protocols may affect editing efficiencies, especial...

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Detalles Bibliográficos
Autores principales: Eggenschwiler, Reto, Gschwendtberger, Thomas, Felski, Christian, Jahn, Christopher, Langer, Florian, Sterneckert, Jared, Hermann, Andreas, Lühmann, Jonathan, Steinemann, Doris, Haase, Alexandra, Martin, Ulrich, Petri, Susanne, Cantz, Tobias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589839/
https://www.ncbi.nlm.nih.gov/pubmed/34773059
http://dx.doi.org/10.1038/s41598-021-01689-2

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