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Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster
Ammonia and its amine-containing derivatives are widely found in natural decomposition byproducts. Here, we conducted biased chemoreceptor screening to investigate the mechanisms by which different concentrations of ammonium salt, urea, and putrescine in rotten fruits affect feeding and oviposition...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589963/ https://www.ncbi.nlm.nih.gov/pubmed/34773080 http://dx.doi.org/10.1038/s42003-021-02799-3 |
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author | Dhakal, Subash Sang, Jiun Aryal, Binod Lee, Youngseok |
author_facet | Dhakal, Subash Sang, Jiun Aryal, Binod Lee, Youngseok |
author_sort | Dhakal, Subash |
collection | PubMed |
description | Ammonia and its amine-containing derivatives are widely found in natural decomposition byproducts. Here, we conducted biased chemoreceptor screening to investigate the mechanisms by which different concentrations of ammonium salt, urea, and putrescine in rotten fruits affect feeding and oviposition behavior. We identified three ionotropic receptors, including the two broadly required IR25a and IR76b receptors, as well as the narrowly tuned IR51b receptor. These three IRs were fundamental in eliciting avoidance against nitrogenous waste products, which is mediated by bitter-sensing gustatory receptor neurons (GRNs). The aversion of nitrogenous wastes was evaluated by the cellular requirement by expressing Kir2.1 and behavioral recoveries of the mutants in bitter-sensing GRNs. Furthermore, by conducting electrophysiology assays, we confirmed that ammonia compounds are aversive in taste as they directly activated bitter-sensing GRNs. Therefore, our findings provide insights into the ecological roles of IRs as a means to detect and avoid toxic nitrogenous waste products in nature. |
format | Online Article Text |
id | pubmed-8589963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85899632021-11-15 Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster Dhakal, Subash Sang, Jiun Aryal, Binod Lee, Youngseok Commun Biol Article Ammonia and its amine-containing derivatives are widely found in natural decomposition byproducts. Here, we conducted biased chemoreceptor screening to investigate the mechanisms by which different concentrations of ammonium salt, urea, and putrescine in rotten fruits affect feeding and oviposition behavior. We identified three ionotropic receptors, including the two broadly required IR25a and IR76b receptors, as well as the narrowly tuned IR51b receptor. These three IRs were fundamental in eliciting avoidance against nitrogenous waste products, which is mediated by bitter-sensing gustatory receptor neurons (GRNs). The aversion of nitrogenous wastes was evaluated by the cellular requirement by expressing Kir2.1 and behavioral recoveries of the mutants in bitter-sensing GRNs. Furthermore, by conducting electrophysiology assays, we confirmed that ammonia compounds are aversive in taste as they directly activated bitter-sensing GRNs. Therefore, our findings provide insights into the ecological roles of IRs as a means to detect and avoid toxic nitrogenous waste products in nature. Nature Publishing Group UK 2021-11-12 /pmc/articles/PMC8589963/ /pubmed/34773080 http://dx.doi.org/10.1038/s42003-021-02799-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Dhakal, Subash Sang, Jiun Aryal, Binod Lee, Youngseok Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster |
title | Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster |
title_full | Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster |
title_fullStr | Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster |
title_full_unstemmed | Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster |
title_short | Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster |
title_sort | ionotropic receptors mediate nitrogenous waste avoidance in drosophila melanogaster |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589963/ https://www.ncbi.nlm.nih.gov/pubmed/34773080 http://dx.doi.org/10.1038/s42003-021-02799-3 |
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