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The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment
Weed resistance to herbicides constitutes a serious problem to world crop production. One of the weeds that are significantly threatening the crops’ yield and quality is Apera spica-venti. The target-site resistance (TSR) mechanism of A. spica-venti has been widely studied, though, little is known a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589970/ https://www.ncbi.nlm.nih.gov/pubmed/34772993 http://dx.doi.org/10.1038/s41598-021-01615-6 |
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author | Wrzesińska, Barbara Kościelniak, Karolina Frąckowiak, Patryk Praczyk, Tadeusz Obrępalska-Stęplowska, Aleksandra |
author_facet | Wrzesińska, Barbara Kościelniak, Karolina Frąckowiak, Patryk Praczyk, Tadeusz Obrępalska-Stęplowska, Aleksandra |
author_sort | Wrzesińska, Barbara |
collection | PubMed |
description | Weed resistance to herbicides constitutes a serious problem to world crop production. One of the weeds that are significantly threatening the crops’ yield and quality is Apera spica-venti. The target-site resistance (TSR) mechanism of A. spica-venti has been widely studied, though, little is known about its non-target-site resistance (NTSR) mechanisms at the molecular level. Molecular examination of NTSR is, to a great extent, based on the expression profiles of selected genes, e.g. those participating in detoxification. However, to obtain reliable results of gene expression analysis, the use of a normalizer is required. The aim of this study was to select the best reference genes in A. spica-venti plants of both populations, susceptible and resistant to ALS inhibitor, under treatment with herbicide. Eleven housekeeping genes were chosen for their expression stability assessment. The efficiency correction of raw quantification cycles (Cq) was included in the gene expression stability analyses, which resulted in indicating the TATA-box binding protein (TBP), glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC), and peptidyl-prolyl cis–trans isomerase CYP28 (CYP28) genes as the most stably expressed reference genes. The obtained results are of vital importance for future studies on the expression of genes associated with the non-target-site resistance mechanisms in the A. spica-venti populations susceptible and resistant to herbicides. |
format | Online Article Text |
id | pubmed-8589970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85899702021-11-16 The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment Wrzesińska, Barbara Kościelniak, Karolina Frąckowiak, Patryk Praczyk, Tadeusz Obrępalska-Stęplowska, Aleksandra Sci Rep Article Weed resistance to herbicides constitutes a serious problem to world crop production. One of the weeds that are significantly threatening the crops’ yield and quality is Apera spica-venti. The target-site resistance (TSR) mechanism of A. spica-venti has been widely studied, though, little is known about its non-target-site resistance (NTSR) mechanisms at the molecular level. Molecular examination of NTSR is, to a great extent, based on the expression profiles of selected genes, e.g. those participating in detoxification. However, to obtain reliable results of gene expression analysis, the use of a normalizer is required. The aim of this study was to select the best reference genes in A. spica-venti plants of both populations, susceptible and resistant to ALS inhibitor, under treatment with herbicide. Eleven housekeeping genes were chosen for their expression stability assessment. The efficiency correction of raw quantification cycles (Cq) was included in the gene expression stability analyses, which resulted in indicating the TATA-box binding protein (TBP), glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC), and peptidyl-prolyl cis–trans isomerase CYP28 (CYP28) genes as the most stably expressed reference genes. The obtained results are of vital importance for future studies on the expression of genes associated with the non-target-site resistance mechanisms in the A. spica-venti populations susceptible and resistant to herbicides. Nature Publishing Group UK 2021-11-12 /pmc/articles/PMC8589970/ /pubmed/34772993 http://dx.doi.org/10.1038/s41598-021-01615-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Wrzesińska, Barbara Kościelniak, Karolina Frąckowiak, Patryk Praczyk, Tadeusz Obrępalska-Stęplowska, Aleksandra The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment |
title | The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment |
title_full | The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment |
title_fullStr | The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment |
title_full_unstemmed | The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment |
title_short | The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment |
title_sort | analysis of reference genes expression stability in susceptible and resistant apera spica-venti populations under herbicide treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589970/ https://www.ncbi.nlm.nih.gov/pubmed/34772993 http://dx.doi.org/10.1038/s41598-021-01615-6 |
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