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The epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction
The epicardial administration of therapeutics via the pericardial sac offers an attractive route, since it is minimally invasive and carries no risks of coronary embolization. The aim of this study was to assess viability, safety and effectiveness of cardiosphere-derived cells (CDCs), their extracel...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590017/ https://www.ncbi.nlm.nih.gov/pubmed/34772964 http://dx.doi.org/10.1038/s41598-021-01728-y |
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author | Crisóstomo, Verónica Baéz-Diaz, Claudia Blanco-Blázquez, Virginia Álvarez, Verónica López-Nieto, Esther Maestre, Juan Bayes-Genis, Antoni Gálvez-Montón, Carolina Casado, Javier G. Sánchez-Margallo, Francisco M. |
author_facet | Crisóstomo, Verónica Baéz-Diaz, Claudia Blanco-Blázquez, Virginia Álvarez, Verónica López-Nieto, Esther Maestre, Juan Bayes-Genis, Antoni Gálvez-Montón, Carolina Casado, Javier G. Sánchez-Margallo, Francisco M. |
author_sort | Crisóstomo, Verónica |
collection | PubMed |
description | The epicardial administration of therapeutics via the pericardial sac offers an attractive route, since it is minimally invasive and carries no risks of coronary embolization. The aim of this study was to assess viability, safety and effectiveness of cardiosphere-derived cells (CDCs), their extracellular vesicles (EVs) or placebo administered via a mini-thoracotomy 72 h after experimental infarction in swine. The epicardial administration was completed successfully in all cases in a surgery time (knife-to-skin) below 30 min. No significant differences between groups were found in cardiac function parameters evaluated using magnetic resonance imaging before therapy and at the end of the study, despite a trend towards improved function in CDC-treated animals. Moreover, infarct size at 10 weeks was smaller in treated animals, albeit not significantly. Arrhythmia inducibility did not differ between groups. Pathological examination showed no differences, nor were there any pericardial adhesions evidenced in any case 10 weeks after surgery. These results show that the epicardial delivery of CDCs or their EVs is safe and technically easy 3 days after experimental myocardial infarction in swine, but it does not appear to have any beneficial effect on cardiac function. Our results do not support clinical translation of these therapies as implemented in this work. |
format | Online Article Text |
id | pubmed-8590017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85900172021-11-16 The epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction Crisóstomo, Verónica Baéz-Diaz, Claudia Blanco-Blázquez, Virginia Álvarez, Verónica López-Nieto, Esther Maestre, Juan Bayes-Genis, Antoni Gálvez-Montón, Carolina Casado, Javier G. Sánchez-Margallo, Francisco M. Sci Rep Article The epicardial administration of therapeutics via the pericardial sac offers an attractive route, since it is minimally invasive and carries no risks of coronary embolization. The aim of this study was to assess viability, safety and effectiveness of cardiosphere-derived cells (CDCs), their extracellular vesicles (EVs) or placebo administered via a mini-thoracotomy 72 h after experimental infarction in swine. The epicardial administration was completed successfully in all cases in a surgery time (knife-to-skin) below 30 min. No significant differences between groups were found in cardiac function parameters evaluated using magnetic resonance imaging before therapy and at the end of the study, despite a trend towards improved function in CDC-treated animals. Moreover, infarct size at 10 weeks was smaller in treated animals, albeit not significantly. Arrhythmia inducibility did not differ between groups. Pathological examination showed no differences, nor were there any pericardial adhesions evidenced in any case 10 weeks after surgery. These results show that the epicardial delivery of CDCs or their EVs is safe and technically easy 3 days after experimental myocardial infarction in swine, but it does not appear to have any beneficial effect on cardiac function. Our results do not support clinical translation of these therapies as implemented in this work. Nature Publishing Group UK 2021-11-12 /pmc/articles/PMC8590017/ /pubmed/34772964 http://dx.doi.org/10.1038/s41598-021-01728-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Crisóstomo, Verónica Baéz-Diaz, Claudia Blanco-Blázquez, Virginia Álvarez, Verónica López-Nieto, Esther Maestre, Juan Bayes-Genis, Antoni Gálvez-Montón, Carolina Casado, Javier G. Sánchez-Margallo, Francisco M. The epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction |
title | The epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction |
title_full | The epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction |
title_fullStr | The epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction |
title_full_unstemmed | The epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction |
title_short | The epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction |
title_sort | epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590017/ https://www.ncbi.nlm.nih.gov/pubmed/34772964 http://dx.doi.org/10.1038/s41598-021-01728-y |
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