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The value of bacterial metagenomic analysis in post-surgical examination of gallstones
Gallstone disease is one of the most common causes of hospitalization for gastrointestinal diseases in the world. Recent studies have examined the presence of bacteria in the formation of stones. Our main goal was to determine the overall composition of gallstone microflora. Gallstones were obtained...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590668/ https://www.ncbi.nlm.nih.gov/pubmed/34562145 http://dx.doi.org/10.1007/s00203-021-02580-4 |
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author | Ploszaj, T. Brauncajs, M. Traczyk-Borszynska, M. Matyjas, T. Pomorski, L. Wasiak, T. Borowiec, M. |
author_facet | Ploszaj, T. Brauncajs, M. Traczyk-Borszynska, M. Matyjas, T. Pomorski, L. Wasiak, T. Borowiec, M. |
author_sort | Ploszaj, T. |
collection | PubMed |
description | Gallstone disease is one of the most common causes of hospitalization for gastrointestinal diseases in the world. Recent studies have examined the presence of bacteria in the formation of stones. Our main goal was to determine the overall composition of gallstone microflora. Gallstones were obtained from 24 patients during laparoscopic cholecystectomy from which DNA were extracted. Composition of bacterial flora was evaluated on 16 s rDNA sequencing technique. In the vast majority of samples, bacteria were present, and four groups could be differentiated regarding the flora. Overall composition shows that 87% of the stones were cholesterol/mixed type of gallstone. Additionally, potentially harmful microorganisms (Streptococcus, Clostridium and Kocuria) that could cause post-surgery complications were identified in several patients. The obtained results indicate that this technique may be useful in analyzing the type of stones and in pinpointing the presence of pathogenic bacteria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00203-021-02580-4. |
format | Online Article Text |
id | pubmed-8590668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-85906682021-11-23 The value of bacterial metagenomic analysis in post-surgical examination of gallstones Ploszaj, T. Brauncajs, M. Traczyk-Borszynska, M. Matyjas, T. Pomorski, L. Wasiak, T. Borowiec, M. Arch Microbiol Short Communication Gallstone disease is one of the most common causes of hospitalization for gastrointestinal diseases in the world. Recent studies have examined the presence of bacteria in the formation of stones. Our main goal was to determine the overall composition of gallstone microflora. Gallstones were obtained from 24 patients during laparoscopic cholecystectomy from which DNA were extracted. Composition of bacterial flora was evaluated on 16 s rDNA sequencing technique. In the vast majority of samples, bacteria were present, and four groups could be differentiated regarding the flora. Overall composition shows that 87% of the stones were cholesterol/mixed type of gallstone. Additionally, potentially harmful microorganisms (Streptococcus, Clostridium and Kocuria) that could cause post-surgery complications were identified in several patients. The obtained results indicate that this technique may be useful in analyzing the type of stones and in pinpointing the presence of pathogenic bacteria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00203-021-02580-4. Springer Berlin Heidelberg 2021-09-25 2021 /pmc/articles/PMC8590668/ /pubmed/34562145 http://dx.doi.org/10.1007/s00203-021-02580-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Short Communication Ploszaj, T. Brauncajs, M. Traczyk-Borszynska, M. Matyjas, T. Pomorski, L. Wasiak, T. Borowiec, M. The value of bacterial metagenomic analysis in post-surgical examination of gallstones |
title | The value of bacterial metagenomic analysis in post-surgical examination of gallstones |
title_full | The value of bacterial metagenomic analysis in post-surgical examination of gallstones |
title_fullStr | The value of bacterial metagenomic analysis in post-surgical examination of gallstones |
title_full_unstemmed | The value of bacterial metagenomic analysis in post-surgical examination of gallstones |
title_short | The value of bacterial metagenomic analysis in post-surgical examination of gallstones |
title_sort | value of bacterial metagenomic analysis in post-surgical examination of gallstones |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590668/ https://www.ncbi.nlm.nih.gov/pubmed/34562145 http://dx.doi.org/10.1007/s00203-021-02580-4 |
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