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Biodegradation of Nicotine and TSNAs by Bacterium sp. Strain J54

BACKGROUND: Microorganisms play an important role in reducing harmful substances in flue-cured tobacco. Numerous studies have been conducted to degrade nicotine by microorganisms. OBJECTIVES: The present research deals with the isolation of a potent bacterial strain able to efficiently degrade nicot...

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Autores principales: Jiang, Yonglei, Gong, Jiangshiqi, Chen, Yi, Hu, Binbin, Sun, Jianfeng, Zhu, Yanmei, Xia, Zhenyuan, Zou, Congming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Genetic Engineering and Biotechnology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590725/
https://www.ncbi.nlm.nih.gov/pubmed/34825016
http://dx.doi.org/10.30498/ijb.2021.240460.2812
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author Jiang, Yonglei
Gong, Jiangshiqi
Chen, Yi
Hu, Binbin
Sun, Jianfeng
Zhu, Yanmei
Xia, Zhenyuan
Zou, Congming
author_facet Jiang, Yonglei
Gong, Jiangshiqi
Chen, Yi
Hu, Binbin
Sun, Jianfeng
Zhu, Yanmei
Xia, Zhenyuan
Zou, Congming
author_sort Jiang, Yonglei
collection PubMed
description BACKGROUND: Microorganisms play an important role in reducing harmful substances in flue-cured tobacco. Numerous studies have been conducted to degrade nicotine by microorganisms. OBJECTIVES: The present research deals with the isolation of a potent bacterial strain able to efficiently degrade nicotine and tobacco-specific nitrosamines (TSNAs) in flue-cured tobacco. MATERIAL AND METHODS: Bacterial strain J54, capable of efficiently degrading nicotine and tobacco-specific nitrosamines (TSNAs), was isolated from tobacco leaves and identified. The strain J54 can use nicotine as the sole carbon and nitrogen source and could effectively degrade nicotine while growing in a nicotine isolation medium (NIM) medium. RESULTS: Compared with the control (CK), the total TSNAs content in the tobacco flue-cured eaves after being sprayed with a solution of the J54 strain was found to decrease by 26.22%. Therein, the degradation rates of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N’-nitrosonornicotine (NNN), N’-nitrosoanatabine (NAT), and N′-nitrosoanabasine (NAB) were 24.01%, 26.27%, 28.6%, and 1.83%, respectively. CONCLUSIONS: Bacterial strain J54, was isolated from tobacco leaves and identified as a bacterium, which is similar to Bacillus altitudinis based on its morphological and biochemical characteristics and by phylogenetic analysis based on 16S rRNA gene sequences. To our knowledge, this is the first report of the isolation and characterization of a Bacillus sp. strain that can efficiently degrade nicotine and TSNAs. The findings pave the way for the application of new biotechnologies for the degradation of nicotine and TSNAs by microorganisms.
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spelling pubmed-85907252021-11-24 Biodegradation of Nicotine and TSNAs by Bacterium sp. Strain J54 Jiang, Yonglei Gong, Jiangshiqi Chen, Yi Hu, Binbin Sun, Jianfeng Zhu, Yanmei Xia, Zhenyuan Zou, Congming Iran J Biotechnol Research Article BACKGROUND: Microorganisms play an important role in reducing harmful substances in flue-cured tobacco. Numerous studies have been conducted to degrade nicotine by microorganisms. OBJECTIVES: The present research deals with the isolation of a potent bacterial strain able to efficiently degrade nicotine and tobacco-specific nitrosamines (TSNAs) in flue-cured tobacco. MATERIAL AND METHODS: Bacterial strain J54, capable of efficiently degrading nicotine and tobacco-specific nitrosamines (TSNAs), was isolated from tobacco leaves and identified. The strain J54 can use nicotine as the sole carbon and nitrogen source and could effectively degrade nicotine while growing in a nicotine isolation medium (NIM) medium. RESULTS: Compared with the control (CK), the total TSNAs content in the tobacco flue-cured eaves after being sprayed with a solution of the J54 strain was found to decrease by 26.22%. Therein, the degradation rates of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N’-nitrosonornicotine (NNN), N’-nitrosoanatabine (NAT), and N′-nitrosoanabasine (NAB) were 24.01%, 26.27%, 28.6%, and 1.83%, respectively. CONCLUSIONS: Bacterial strain J54, was isolated from tobacco leaves and identified as a bacterium, which is similar to Bacillus altitudinis based on its morphological and biochemical characteristics and by phylogenetic analysis based on 16S rRNA gene sequences. To our knowledge, this is the first report of the isolation and characterization of a Bacillus sp. strain that can efficiently degrade nicotine and TSNAs. The findings pave the way for the application of new biotechnologies for the degradation of nicotine and TSNAs by microorganisms. National Institute of Genetic Engineering and Biotechnology 2021-07-01 /pmc/articles/PMC8590725/ /pubmed/34825016 http://dx.doi.org/10.30498/ijb.2021.240460.2812 Text en Copyright: © 2021 The Author(s); Published by Iranian Journal of Biotechnology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 Unported License, ( http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jiang, Yonglei
Gong, Jiangshiqi
Chen, Yi
Hu, Binbin
Sun, Jianfeng
Zhu, Yanmei
Xia, Zhenyuan
Zou, Congming
Biodegradation of Nicotine and TSNAs by Bacterium sp. Strain J54
title Biodegradation of Nicotine and TSNAs by Bacterium sp. Strain J54
title_full Biodegradation of Nicotine and TSNAs by Bacterium sp. Strain J54
title_fullStr Biodegradation of Nicotine and TSNAs by Bacterium sp. Strain J54
title_full_unstemmed Biodegradation of Nicotine and TSNAs by Bacterium sp. Strain J54
title_short Biodegradation of Nicotine and TSNAs by Bacterium sp. Strain J54
title_sort biodegradation of nicotine and tsnas by bacterium sp. strain j54
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590725/
https://www.ncbi.nlm.nih.gov/pubmed/34825016
http://dx.doi.org/10.30498/ijb.2021.240460.2812
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