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TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion
Successful implantation requires the coordinated migration and invasion of trophoblast cells from out of the blastocyst and into the endometrium. This process relies on signals produced by cells in the maternal endometrium. However, the relative contribution of stroma cells remains unclear. The stud...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591203/ https://www.ncbi.nlm.nih.gov/pubmed/34790194 http://dx.doi.org/10.3389/fimmu.2021.737401 |
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author | You, Yuan Stelzl, Patrick Joseph, Dana N. Aldo, Paulomi B. Maxwell, Anthony J. Dekel, Nava Liao, Aihua Whirledge, Shannon Mor, Gil |
author_facet | You, Yuan Stelzl, Patrick Joseph, Dana N. Aldo, Paulomi B. Maxwell, Anthony J. Dekel, Nava Liao, Aihua Whirledge, Shannon Mor, Gil |
author_sort | You, Yuan |
collection | PubMed |
description | Successful implantation requires the coordinated migration and invasion of trophoblast cells from out of the blastocyst and into the endometrium. This process relies on signals produced by cells in the maternal endometrium. However, the relative contribution of stroma cells remains unclear. The study of human implantation has major technical limitations, therefore the need of in vitro models to elucidate the molecular mechanisms. Using a recently described 3D in vitro models we evaluated the interaction between trophoblasts and human endometrial stroma cells (hESC), we assessed the process of trophoblast migration and invasion in the presence of stroma derived factors. We demonstrate that hESC promotes trophoblast invasion through the generation of an inflammatory environment modulated by TNF-α. We also show the role of stromal derived IL-17 as a promoter of trophoblast migration through the induction of essential genes that confer invasive capacity to cells of the trophectoderm. In conclusion, we describe the characterization of a cellular inflammatory network that may be important for blastocyst implantation. Our findings provide a new insight into the complexity of the implantation process and reveal the importance of inflammation for embryo implantation. |
format | Online Article Text |
id | pubmed-8591203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85912032021-11-16 TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion You, Yuan Stelzl, Patrick Joseph, Dana N. Aldo, Paulomi B. Maxwell, Anthony J. Dekel, Nava Liao, Aihua Whirledge, Shannon Mor, Gil Front Immunol Immunology Successful implantation requires the coordinated migration and invasion of trophoblast cells from out of the blastocyst and into the endometrium. This process relies on signals produced by cells in the maternal endometrium. However, the relative contribution of stroma cells remains unclear. The study of human implantation has major technical limitations, therefore the need of in vitro models to elucidate the molecular mechanisms. Using a recently described 3D in vitro models we evaluated the interaction between trophoblasts and human endometrial stroma cells (hESC), we assessed the process of trophoblast migration and invasion in the presence of stroma derived factors. We demonstrate that hESC promotes trophoblast invasion through the generation of an inflammatory environment modulated by TNF-α. We also show the role of stromal derived IL-17 as a promoter of trophoblast migration through the induction of essential genes that confer invasive capacity to cells of the trophectoderm. In conclusion, we describe the characterization of a cellular inflammatory network that may be important for blastocyst implantation. Our findings provide a new insight into the complexity of the implantation process and reveal the importance of inflammation for embryo implantation. Frontiers Media S.A. 2021-11-01 /pmc/articles/PMC8591203/ /pubmed/34790194 http://dx.doi.org/10.3389/fimmu.2021.737401 Text en Copyright © 2021 You, Stelzl, Joseph, Aldo, Maxwell, Dekel, Liao, Whirledge and Mor https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology You, Yuan Stelzl, Patrick Joseph, Dana N. Aldo, Paulomi B. Maxwell, Anthony J. Dekel, Nava Liao, Aihua Whirledge, Shannon Mor, Gil TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion |
title | TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion |
title_full | TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion |
title_fullStr | TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion |
title_full_unstemmed | TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion |
title_short | TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion |
title_sort | tnf-α regulated endometrial stroma secretome promotes trophoblast invasion |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591203/ https://www.ncbi.nlm.nih.gov/pubmed/34790194 http://dx.doi.org/10.3389/fimmu.2021.737401 |
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