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TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion

Successful implantation requires the coordinated migration and invasion of trophoblast cells from out of the blastocyst and into the endometrium. This process relies on signals produced by cells in the maternal endometrium. However, the relative contribution of stroma cells remains unclear. The stud...

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Autores principales: You, Yuan, Stelzl, Patrick, Joseph, Dana N., Aldo, Paulomi B., Maxwell, Anthony J., Dekel, Nava, Liao, Aihua, Whirledge, Shannon, Mor, Gil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591203/
https://www.ncbi.nlm.nih.gov/pubmed/34790194
http://dx.doi.org/10.3389/fimmu.2021.737401
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author You, Yuan
Stelzl, Patrick
Joseph, Dana N.
Aldo, Paulomi B.
Maxwell, Anthony J.
Dekel, Nava
Liao, Aihua
Whirledge, Shannon
Mor, Gil
author_facet You, Yuan
Stelzl, Patrick
Joseph, Dana N.
Aldo, Paulomi B.
Maxwell, Anthony J.
Dekel, Nava
Liao, Aihua
Whirledge, Shannon
Mor, Gil
author_sort You, Yuan
collection PubMed
description Successful implantation requires the coordinated migration and invasion of trophoblast cells from out of the blastocyst and into the endometrium. This process relies on signals produced by cells in the maternal endometrium. However, the relative contribution of stroma cells remains unclear. The study of human implantation has major technical limitations, therefore the need of in vitro models to elucidate the molecular mechanisms. Using a recently described 3D in vitro models we evaluated the interaction between trophoblasts and human endometrial stroma cells (hESC), we assessed the process of trophoblast migration and invasion in the presence of stroma derived factors. We demonstrate that hESC promotes trophoblast invasion through the generation of an inflammatory environment modulated by TNF-α. We also show the role of stromal derived IL-17 as a promoter of trophoblast migration through the induction of essential genes that confer invasive capacity to cells of the trophectoderm. In conclusion, we describe the characterization of a cellular inflammatory network that may be important for blastocyst implantation. Our findings provide a new insight into the complexity of the implantation process and reveal the importance of inflammation for embryo implantation.
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spelling pubmed-85912032021-11-16 TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion You, Yuan Stelzl, Patrick Joseph, Dana N. Aldo, Paulomi B. Maxwell, Anthony J. Dekel, Nava Liao, Aihua Whirledge, Shannon Mor, Gil Front Immunol Immunology Successful implantation requires the coordinated migration and invasion of trophoblast cells from out of the blastocyst and into the endometrium. This process relies on signals produced by cells in the maternal endometrium. However, the relative contribution of stroma cells remains unclear. The study of human implantation has major technical limitations, therefore the need of in vitro models to elucidate the molecular mechanisms. Using a recently described 3D in vitro models we evaluated the interaction between trophoblasts and human endometrial stroma cells (hESC), we assessed the process of trophoblast migration and invasion in the presence of stroma derived factors. We demonstrate that hESC promotes trophoblast invasion through the generation of an inflammatory environment modulated by TNF-α. We also show the role of stromal derived IL-17 as a promoter of trophoblast migration through the induction of essential genes that confer invasive capacity to cells of the trophectoderm. In conclusion, we describe the characterization of a cellular inflammatory network that may be important for blastocyst implantation. Our findings provide a new insight into the complexity of the implantation process and reveal the importance of inflammation for embryo implantation. Frontiers Media S.A. 2021-11-01 /pmc/articles/PMC8591203/ /pubmed/34790194 http://dx.doi.org/10.3389/fimmu.2021.737401 Text en Copyright © 2021 You, Stelzl, Joseph, Aldo, Maxwell, Dekel, Liao, Whirledge and Mor https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
You, Yuan
Stelzl, Patrick
Joseph, Dana N.
Aldo, Paulomi B.
Maxwell, Anthony J.
Dekel, Nava
Liao, Aihua
Whirledge, Shannon
Mor, Gil
TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion
title TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion
title_full TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion
title_fullStr TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion
title_full_unstemmed TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion
title_short TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion
title_sort tnf-α regulated endometrial stroma secretome promotes trophoblast invasion
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591203/
https://www.ncbi.nlm.nih.gov/pubmed/34790194
http://dx.doi.org/10.3389/fimmu.2021.737401
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