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Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR

Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli. As E. albertii sometimes causes outbreaks of gastroenteritis, rapid strain typing systems, such as the O- and H-serotyping systems widely used for E. coli, will be useful for outbreak inve...

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Autores principales: Nakae, Koji, Ooka, Tadasuke, Murakami, Koichi, Hara-Kudo, Yukiko, Imuta, Naoko, Gotoh, Yasuhiro, Ogura, Yoshitoshi, Hayashi, Tetsuya, Okamoto, Yasuhiro, Nishi, Junichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591213/
https://www.ncbi.nlm.nih.gov/pubmed/34790177
http://dx.doi.org/10.3389/fmicb.2021.737979
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author Nakae, Koji
Ooka, Tadasuke
Murakami, Koichi
Hara-Kudo, Yukiko
Imuta, Naoko
Gotoh, Yasuhiro
Ogura, Yoshitoshi
Hayashi, Tetsuya
Okamoto, Yasuhiro
Nishi, Junichiro
author_facet Nakae, Koji
Ooka, Tadasuke
Murakami, Koichi
Hara-Kudo, Yukiko
Imuta, Naoko
Gotoh, Yasuhiro
Ogura, Yoshitoshi
Hayashi, Tetsuya
Okamoto, Yasuhiro
Nishi, Junichiro
author_sort Nakae, Koji
collection PubMed
description Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli. As E. albertii sometimes causes outbreaks of gastroenteritis, rapid strain typing systems, such as the O- and H-serotyping systems widely used for E. coli, will be useful for outbreak investigation and surveillance. Although an O-genotyping system has recently been developed, the diversity of E. albertii H-antigens (flagellins) encoded by fliC genes remains to be systematically investigated, and no H-serotyping or genotyping system is currently available. Here, we analyzed the fliC genes of 243 genome-sequenced E. albertii strains and identified 73 sequence types, which were grouped into four clearly distinguishable types designated E. albertii H-genotypes 1–4 (EAHg1–EAHg4). Although there was a clear sign of intraspecies transfer of fliC genes in E. albertii, none of the four E. albertii H-genotypes (EAHgs) were closely related to any of the 53 known E. coli H-antigens, indicating the absence or rare occurrence of interspecies transfer of fliC genes between the two species. Although the analysis of more E. albertii strains will be required to confirm the low level of variation in their fliC genes, this finding suggests that E. albertii may exist in limited natural hosts or environments and/or that the flagella of E. albertii may function in a limited stage(s) in their life cycle. Based on the fliC sequences of the four EAHgs, we developed a multiplex PCR-based H-genotyping system for E. albertii (EAH-genotyping PCR), which will be useful for epidemiological studies of E. albertii infections.
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spelling pubmed-85912132021-11-16 Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR Nakae, Koji Ooka, Tadasuke Murakami, Koichi Hara-Kudo, Yukiko Imuta, Naoko Gotoh, Yasuhiro Ogura, Yoshitoshi Hayashi, Tetsuya Okamoto, Yasuhiro Nishi, Junichiro Front Microbiol Microbiology Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli. As E. albertii sometimes causes outbreaks of gastroenteritis, rapid strain typing systems, such as the O- and H-serotyping systems widely used for E. coli, will be useful for outbreak investigation and surveillance. Although an O-genotyping system has recently been developed, the diversity of E. albertii H-antigens (flagellins) encoded by fliC genes remains to be systematically investigated, and no H-serotyping or genotyping system is currently available. Here, we analyzed the fliC genes of 243 genome-sequenced E. albertii strains and identified 73 sequence types, which were grouped into four clearly distinguishable types designated E. albertii H-genotypes 1–4 (EAHg1–EAHg4). Although there was a clear sign of intraspecies transfer of fliC genes in E. albertii, none of the four E. albertii H-genotypes (EAHgs) were closely related to any of the 53 known E. coli H-antigens, indicating the absence or rare occurrence of interspecies transfer of fliC genes between the two species. Although the analysis of more E. albertii strains will be required to confirm the low level of variation in their fliC genes, this finding suggests that E. albertii may exist in limited natural hosts or environments and/or that the flagella of E. albertii may function in a limited stage(s) in their life cycle. Based on the fliC sequences of the four EAHgs, we developed a multiplex PCR-based H-genotyping system for E. albertii (EAH-genotyping PCR), which will be useful for epidemiological studies of E. albertii infections. Frontiers Media S.A. 2021-11-01 /pmc/articles/PMC8591213/ /pubmed/34790177 http://dx.doi.org/10.3389/fmicb.2021.737979 Text en Copyright © 2021 Nakae, Ooka, Murakami, Hara-Kudo, Imuta, Gotoh, Ogura, Hayashi, Okamoto and Nishi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Nakae, Koji
Ooka, Tadasuke
Murakami, Koichi
Hara-Kudo, Yukiko
Imuta, Naoko
Gotoh, Yasuhiro
Ogura, Yoshitoshi
Hayashi, Tetsuya
Okamoto, Yasuhiro
Nishi, Junichiro
Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR
title Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR
title_full Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR
title_fullStr Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR
title_full_unstemmed Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR
title_short Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR
title_sort diversification of escherichia albertii h-antigens and development of h-genotyping pcr
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591213/
https://www.ncbi.nlm.nih.gov/pubmed/34790177
http://dx.doi.org/10.3389/fmicb.2021.737979
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