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Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration
Bone regeneration is a delicate physiological process. Non-union and delayed fracture healing remains a great challenge in clinical practice nowadays. Bone and fat hold a close relationship to remain balanced through hormones and cytokines. Adiponectin is a well-known protein to maintain the hemosta...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591230/ https://www.ncbi.nlm.nih.gov/pubmed/34790669 http://dx.doi.org/10.3389/fcell.2021.762335 |
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author | Gong, Yanping Wang, Yang Zhang, Yiqing Wang, Liangchen Wan, Lijuan Zu, Yuan Li, Chunlin Wang, Xin Cui, Zhong-Kai |
author_facet | Gong, Yanping Wang, Yang Zhang, Yiqing Wang, Liangchen Wan, Lijuan Zu, Yuan Li, Chunlin Wang, Xin Cui, Zhong-Kai |
author_sort | Gong, Yanping |
collection | PubMed |
description | Bone regeneration is a delicate physiological process. Non-union and delayed fracture healing remains a great challenge in clinical practice nowadays. Bone and fat hold a close relationship to remain balanced through hormones and cytokines. Adiponectin is a well-known protein to maintain the hemostasis, which may be an interesting target for fracture healing. Herein, we provided a facile and efficient method to obtain high-purity and high-yield recombinant human adiponectin (ADPN). The biocompatibility and the pharmaceutical behaviors were evaluated in Sprague–Dawley rats. The paracrine effects of adiponectin on bone fracture healing were investigated with a rat tibia fracture model via intrabone injection. Significantly accelerated bone healing was observed in the medulla injection group, indicating the paracrine effects of adiponectin could be potentially utilized for clinical treatments. The underlying mechanism was primarily assessed, and the expression of osteogenic markers, including bone morphogenic protein 2, alkaline phosphatase, and osteocalcin, along with adiponectin receptor 1 (AdipoR1), was markedly increased at the fracture site. The increased bone healing of ADPN treatment may result from both enhanced osteogenic proliferation as well as differentiation. Cell experiments confirmed that the expression of osteogenesis markers increased significantly in ADPN treatment groups, while it decreased when the expression of AdipoR1 was knocked down by siRNA. Our study provided a feasible and efficacious way for bone fracture treatment with local administration of ADPN, which could be rapidly translated into the clinics. |
format | Online Article Text |
id | pubmed-8591230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-85912302021-11-16 Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration Gong, Yanping Wang, Yang Zhang, Yiqing Wang, Liangchen Wan, Lijuan Zu, Yuan Li, Chunlin Wang, Xin Cui, Zhong-Kai Front Cell Dev Biol Cell and Developmental Biology Bone regeneration is a delicate physiological process. Non-union and delayed fracture healing remains a great challenge in clinical practice nowadays. Bone and fat hold a close relationship to remain balanced through hormones and cytokines. Adiponectin is a well-known protein to maintain the hemostasis, which may be an interesting target for fracture healing. Herein, we provided a facile and efficient method to obtain high-purity and high-yield recombinant human adiponectin (ADPN). The biocompatibility and the pharmaceutical behaviors were evaluated in Sprague–Dawley rats. The paracrine effects of adiponectin on bone fracture healing were investigated with a rat tibia fracture model via intrabone injection. Significantly accelerated bone healing was observed in the medulla injection group, indicating the paracrine effects of adiponectin could be potentially utilized for clinical treatments. The underlying mechanism was primarily assessed, and the expression of osteogenic markers, including bone morphogenic protein 2, alkaline phosphatase, and osteocalcin, along with adiponectin receptor 1 (AdipoR1), was markedly increased at the fracture site. The increased bone healing of ADPN treatment may result from both enhanced osteogenic proliferation as well as differentiation. Cell experiments confirmed that the expression of osteogenesis markers increased significantly in ADPN treatment groups, while it decreased when the expression of AdipoR1 was knocked down by siRNA. Our study provided a feasible and efficacious way for bone fracture treatment with local administration of ADPN, which could be rapidly translated into the clinics. Frontiers Media S.A. 2021-11-01 /pmc/articles/PMC8591230/ /pubmed/34790669 http://dx.doi.org/10.3389/fcell.2021.762335 Text en Copyright © 2021 Gong, Wang, Zhang, Wang, Wan, Zu, Li, Wang and Cui. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Gong, Yanping Wang, Yang Zhang, Yiqing Wang, Liangchen Wan, Lijuan Zu, Yuan Li, Chunlin Wang, Xin Cui, Zhong-Kai Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration |
title | Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration |
title_full | Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration |
title_fullStr | Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration |
title_full_unstemmed | Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration |
title_short | Paracrine Effects of Recombinant Human Adiponectin Promote Bone Regeneration |
title_sort | paracrine effects of recombinant human adiponectin promote bone regeneration |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591230/ https://www.ncbi.nlm.nih.gov/pubmed/34790669 http://dx.doi.org/10.3389/fcell.2021.762335 |
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