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Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease

Wilt disease of roselle (Hibiscus sabdariffa L.) is common in Taiwan; however, the causative agent remains unknown. The stems of wilted roselle are browned, slightly constricted, and covered by white aerial hyphae, suggesting that potential pathogens may originate from soil. To identify the potentia...

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Autores principales: Wang, Chih-Wei, Yu, Yu-Hsiang, Wu, Chun-Yao, Feng, Ru-Ying, Tandon, Kshitij, Chen, Ying-Lien, Tang, Sen-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591290/
https://www.ncbi.nlm.nih.gov/pubmed/34790182
http://dx.doi.org/10.3389/fmicb.2021.756100
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author Wang, Chih-Wei
Yu, Yu-Hsiang
Wu, Chun-Yao
Feng, Ru-Ying
Tandon, Kshitij
Chen, Ying-Lien
Tang, Sen-Lin
author_facet Wang, Chih-Wei
Yu, Yu-Hsiang
Wu, Chun-Yao
Feng, Ru-Ying
Tandon, Kshitij
Chen, Ying-Lien
Tang, Sen-Lin
author_sort Wang, Chih-Wei
collection PubMed
description Wilt disease of roselle (Hibiscus sabdariffa L.) is common in Taiwan; however, the causative agent remains unknown. The stems of wilted roselle are browned, slightly constricted, and covered by white aerial hyphae, suggesting that potential pathogens may originate from soil. To identify the potential pathogens, we conducted a rhizosphere microbiota survey in phenotypically healthy and diseased plants through fungal internal transcribed spacer (ITS) and bacterial 16S rRNA amplicon sequencing for uncovering the microbial compositions in the roselle rhizosphere. The fungal family Nectriaceae exhibited significantly higher abundance in diseased rhizospheres than in healthy rhizospheres, and this bacterial community was more specific to geography (i.e., plot-dependent) than to rhizosphere disease status. However, a few bacterial groups such as Bacilli were associated with the healthy rhizosphere. Fusarium species were the most dominant species of Nectriaceae in the survey and became the main target for potential pathogen isolation. We successfully isolated 119 strains from diseased plants in roselle fields. Koch’s postulates were used to evaluate the pathogenicity of these strains; our results indicated that Fusarium solani K1 (FsK1) can cause wilting and a rotted pith in roselles, which was consistent with observations in the fields. This is the first demonstration that F. solani can cause roselle wilt in Taiwan. Furthermore, these newly isolated strains are the most dominant operational taxonomic units detected in ITS amplicon sequencing in diseased rhizospheres, which serves as further evidence that F. solani is the main pathogen causing the roselle wilt disease. Administration of Bacillus velezensis SOI-3374, a strain isolated from a healthy roselle rhizosphere, caused considerable anti-FsK1 activity, and it can serve as a potential biocontrol agent against roselle wilt disease.
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spelling pubmed-85912902021-11-16 Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease Wang, Chih-Wei Yu, Yu-Hsiang Wu, Chun-Yao Feng, Ru-Ying Tandon, Kshitij Chen, Ying-Lien Tang, Sen-Lin Front Microbiol Microbiology Wilt disease of roselle (Hibiscus sabdariffa L.) is common in Taiwan; however, the causative agent remains unknown. The stems of wilted roselle are browned, slightly constricted, and covered by white aerial hyphae, suggesting that potential pathogens may originate from soil. To identify the potential pathogens, we conducted a rhizosphere microbiota survey in phenotypically healthy and diseased plants through fungal internal transcribed spacer (ITS) and bacterial 16S rRNA amplicon sequencing for uncovering the microbial compositions in the roselle rhizosphere. The fungal family Nectriaceae exhibited significantly higher abundance in diseased rhizospheres than in healthy rhizospheres, and this bacterial community was more specific to geography (i.e., plot-dependent) than to rhizosphere disease status. However, a few bacterial groups such as Bacilli were associated with the healthy rhizosphere. Fusarium species were the most dominant species of Nectriaceae in the survey and became the main target for potential pathogen isolation. We successfully isolated 119 strains from diseased plants in roselle fields. Koch’s postulates were used to evaluate the pathogenicity of these strains; our results indicated that Fusarium solani K1 (FsK1) can cause wilting and a rotted pith in roselles, which was consistent with observations in the fields. This is the first demonstration that F. solani can cause roselle wilt in Taiwan. Furthermore, these newly isolated strains are the most dominant operational taxonomic units detected in ITS amplicon sequencing in diseased rhizospheres, which serves as further evidence that F. solani is the main pathogen causing the roselle wilt disease. Administration of Bacillus velezensis SOI-3374, a strain isolated from a healthy roselle rhizosphere, caused considerable anti-FsK1 activity, and it can serve as a potential biocontrol agent against roselle wilt disease. Frontiers Media S.A. 2021-11-01 /pmc/articles/PMC8591290/ /pubmed/34790182 http://dx.doi.org/10.3389/fmicb.2021.756100 Text en Copyright © 2021 Wang, Yu, Wu, Feng, Tandon, Chen and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Chih-Wei
Yu, Yu-Hsiang
Wu, Chun-Yao
Feng, Ru-Ying
Tandon, Kshitij
Chen, Ying-Lien
Tang, Sen-Lin
Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease
title Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease
title_full Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease
title_fullStr Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease
title_full_unstemmed Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease
title_short Detection of Pathogenic and Beneficial Microbes for Roselle Wilt Disease
title_sort detection of pathogenic and beneficial microbes for roselle wilt disease
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591290/
https://www.ncbi.nlm.nih.gov/pubmed/34790182
http://dx.doi.org/10.3389/fmicb.2021.756100
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