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Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone

Examining the role of environmental enteric dysfunction (EED) in child growth requires noninvasive, field-appropriate biomarkers. Alternatives to the traditionally used lactulose:mannitol (L:M) test have been explored, but few studies have compared the L:M test to host fecal mRNA transcripts. The ob...

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Autores principales: Singh, Akriti, Potani, Isabel, Griswold, Stacy P., Suri, Devika, Langlois, Breanne, Shen, Ye, Walton, Shelley M., Kwan Ho Chui, Kenneth, Manary, Mark J., Webb, Patrick, Rogers, Beatrice L., Rosenberg, Irwin H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592211/
https://www.ncbi.nlm.nih.gov/pubmed/34460425
http://dx.doi.org/10.4269/ajtmh.21-0348
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author Singh, Akriti
Potani, Isabel
Griswold, Stacy P.
Suri, Devika
Langlois, Breanne
Shen, Ye
Walton, Shelley M.
Kwan Ho Chui, Kenneth
Manary, Mark J.
Webb, Patrick
Rogers, Beatrice L.
Rosenberg, Irwin H.
author_facet Singh, Akriti
Potani, Isabel
Griswold, Stacy P.
Suri, Devika
Langlois, Breanne
Shen, Ye
Walton, Shelley M.
Kwan Ho Chui, Kenneth
Manary, Mark J.
Webb, Patrick
Rogers, Beatrice L.
Rosenberg, Irwin H.
author_sort Singh, Akriti
collection PubMed
description Examining the role of environmental enteric dysfunction (EED) in child growth requires noninvasive, field-appropriate biomarkers. Alternatives to the traditionally used lactulose:mannitol (L:M) test have been explored, but few studies have compared the L:M test to host fecal mRNA transcripts. The objectives of this study were to examine whether 1) host fecal mRNA transcripts could predict presence and severity of EED, measured using the L:M test, and 2) EED modifies the effect of specialized nutritious foods (SNFs) on recovery from moderate acute malnutrition (MAM). This substudy was nested within a cluster randomized trial comparing four SNFs in the treatment of MAM among children 6 to 59 months in Sierra Leone. EED was assessed at enrollment using the L:M test and 15 host fecal mRNA transcripts on 522 children. Recovery from MAM was defined as achieving mid-upper arm circumference ≥ 12.5 cm within 12 weeks of supplementation. Random forest classification models were used to examine prediction of presence and severity of EED by host fecal mRNA transcripts. Logistic regression was used to test for effect modification by L:M test variables including % lactulose excreted (%L). Eight host fecal mRNA transcripts (AQP9, REG3A, IFI30, DECR1, BIRC3, SELL, PIK3AP1, DEFA6) identified EED (%L ≥ 0.2) and severe EED (%L ≥ 0.45) with high sensitivity and specificity. The L:M test variables did not modify the effect of SNFs on recovery from MAM. In this study, we found host fecal mRNA transcripts that could be biomarkers of EED but did not find EED to modify the effect of SNFs on MAM treatment.
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spelling pubmed-85922112021-11-24 Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone Singh, Akriti Potani, Isabel Griswold, Stacy P. Suri, Devika Langlois, Breanne Shen, Ye Walton, Shelley M. Kwan Ho Chui, Kenneth Manary, Mark J. Webb, Patrick Rogers, Beatrice L. Rosenberg, Irwin H. Am J Trop Med Hyg Article Examining the role of environmental enteric dysfunction (EED) in child growth requires noninvasive, field-appropriate biomarkers. Alternatives to the traditionally used lactulose:mannitol (L:M) test have been explored, but few studies have compared the L:M test to host fecal mRNA transcripts. The objectives of this study were to examine whether 1) host fecal mRNA transcripts could predict presence and severity of EED, measured using the L:M test, and 2) EED modifies the effect of specialized nutritious foods (SNFs) on recovery from moderate acute malnutrition (MAM). This substudy was nested within a cluster randomized trial comparing four SNFs in the treatment of MAM among children 6 to 59 months in Sierra Leone. EED was assessed at enrollment using the L:M test and 15 host fecal mRNA transcripts on 522 children. Recovery from MAM was defined as achieving mid-upper arm circumference ≥ 12.5 cm within 12 weeks of supplementation. Random forest classification models were used to examine prediction of presence and severity of EED by host fecal mRNA transcripts. Logistic regression was used to test for effect modification by L:M test variables including % lactulose excreted (%L). Eight host fecal mRNA transcripts (AQP9, REG3A, IFI30, DECR1, BIRC3, SELL, PIK3AP1, DEFA6) identified EED (%L ≥ 0.2) and severe EED (%L ≥ 0.45) with high sensitivity and specificity. The L:M test variables did not modify the effect of SNFs on recovery from MAM. In this study, we found host fecal mRNA transcripts that could be biomarkers of EED but did not find EED to modify the effect of SNFs on MAM treatment. The American Society of Tropical Medicine and Hygiene 2021-11 2021-08-30 /pmc/articles/PMC8592211/ /pubmed/34460425 http://dx.doi.org/10.4269/ajtmh.21-0348 Text en © The American Society of Tropical Medicine and Hygiene https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Singh, Akriti
Potani, Isabel
Griswold, Stacy P.
Suri, Devika
Langlois, Breanne
Shen, Ye
Walton, Shelley M.
Kwan Ho Chui, Kenneth
Manary, Mark J.
Webb, Patrick
Rogers, Beatrice L.
Rosenberg, Irwin H.
Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone
title Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone
title_full Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone
title_fullStr Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone
title_full_unstemmed Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone
title_short Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone
title_sort host fecal mrnas predicted environmental enteric dysfunction among children with moderate acute malnutrition in sierra leone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592211/
https://www.ncbi.nlm.nih.gov/pubmed/34460425
http://dx.doi.org/10.4269/ajtmh.21-0348
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