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Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone
Examining the role of environmental enteric dysfunction (EED) in child growth requires noninvasive, field-appropriate biomarkers. Alternatives to the traditionally used lactulose:mannitol (L:M) test have been explored, but few studies have compared the L:M test to host fecal mRNA transcripts. The ob...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The American Society of Tropical Medicine and Hygiene
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592211/ https://www.ncbi.nlm.nih.gov/pubmed/34460425 http://dx.doi.org/10.4269/ajtmh.21-0348 |
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author | Singh, Akriti Potani, Isabel Griswold, Stacy P. Suri, Devika Langlois, Breanne Shen, Ye Walton, Shelley M. Kwan Ho Chui, Kenneth Manary, Mark J. Webb, Patrick Rogers, Beatrice L. Rosenberg, Irwin H. |
author_facet | Singh, Akriti Potani, Isabel Griswold, Stacy P. Suri, Devika Langlois, Breanne Shen, Ye Walton, Shelley M. Kwan Ho Chui, Kenneth Manary, Mark J. Webb, Patrick Rogers, Beatrice L. Rosenberg, Irwin H. |
author_sort | Singh, Akriti |
collection | PubMed |
description | Examining the role of environmental enteric dysfunction (EED) in child growth requires noninvasive, field-appropriate biomarkers. Alternatives to the traditionally used lactulose:mannitol (L:M) test have been explored, but few studies have compared the L:M test to host fecal mRNA transcripts. The objectives of this study were to examine whether 1) host fecal mRNA transcripts could predict presence and severity of EED, measured using the L:M test, and 2) EED modifies the effect of specialized nutritious foods (SNFs) on recovery from moderate acute malnutrition (MAM). This substudy was nested within a cluster randomized trial comparing four SNFs in the treatment of MAM among children 6 to 59 months in Sierra Leone. EED was assessed at enrollment using the L:M test and 15 host fecal mRNA transcripts on 522 children. Recovery from MAM was defined as achieving mid-upper arm circumference ≥ 12.5 cm within 12 weeks of supplementation. Random forest classification models were used to examine prediction of presence and severity of EED by host fecal mRNA transcripts. Logistic regression was used to test for effect modification by L:M test variables including % lactulose excreted (%L). Eight host fecal mRNA transcripts (AQP9, REG3A, IFI30, DECR1, BIRC3, SELL, PIK3AP1, DEFA6) identified EED (%L ≥ 0.2) and severe EED (%L ≥ 0.45) with high sensitivity and specificity. The L:M test variables did not modify the effect of SNFs on recovery from MAM. In this study, we found host fecal mRNA transcripts that could be biomarkers of EED but did not find EED to modify the effect of SNFs on MAM treatment. |
format | Online Article Text |
id | pubmed-8592211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The American Society of Tropical Medicine and Hygiene |
record_format | MEDLINE/PubMed |
spelling | pubmed-85922112021-11-24 Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone Singh, Akriti Potani, Isabel Griswold, Stacy P. Suri, Devika Langlois, Breanne Shen, Ye Walton, Shelley M. Kwan Ho Chui, Kenneth Manary, Mark J. Webb, Patrick Rogers, Beatrice L. Rosenberg, Irwin H. Am J Trop Med Hyg Article Examining the role of environmental enteric dysfunction (EED) in child growth requires noninvasive, field-appropriate biomarkers. Alternatives to the traditionally used lactulose:mannitol (L:M) test have been explored, but few studies have compared the L:M test to host fecal mRNA transcripts. The objectives of this study were to examine whether 1) host fecal mRNA transcripts could predict presence and severity of EED, measured using the L:M test, and 2) EED modifies the effect of specialized nutritious foods (SNFs) on recovery from moderate acute malnutrition (MAM). This substudy was nested within a cluster randomized trial comparing four SNFs in the treatment of MAM among children 6 to 59 months in Sierra Leone. EED was assessed at enrollment using the L:M test and 15 host fecal mRNA transcripts on 522 children. Recovery from MAM was defined as achieving mid-upper arm circumference ≥ 12.5 cm within 12 weeks of supplementation. Random forest classification models were used to examine prediction of presence and severity of EED by host fecal mRNA transcripts. Logistic regression was used to test for effect modification by L:M test variables including % lactulose excreted (%L). Eight host fecal mRNA transcripts (AQP9, REG3A, IFI30, DECR1, BIRC3, SELL, PIK3AP1, DEFA6) identified EED (%L ≥ 0.2) and severe EED (%L ≥ 0.45) with high sensitivity and specificity. The L:M test variables did not modify the effect of SNFs on recovery from MAM. In this study, we found host fecal mRNA transcripts that could be biomarkers of EED but did not find EED to modify the effect of SNFs on MAM treatment. The American Society of Tropical Medicine and Hygiene 2021-11 2021-08-30 /pmc/articles/PMC8592211/ /pubmed/34460425 http://dx.doi.org/10.4269/ajtmh.21-0348 Text en © The American Society of Tropical Medicine and Hygiene https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Article Singh, Akriti Potani, Isabel Griswold, Stacy P. Suri, Devika Langlois, Breanne Shen, Ye Walton, Shelley M. Kwan Ho Chui, Kenneth Manary, Mark J. Webb, Patrick Rogers, Beatrice L. Rosenberg, Irwin H. Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone |
title | Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone |
title_full | Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone |
title_fullStr | Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone |
title_full_unstemmed | Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone |
title_short | Host Fecal mRNAs Predicted Environmental Enteric Dysfunction among Children with Moderate Acute Malnutrition in Sierra Leone |
title_sort | host fecal mrnas predicted environmental enteric dysfunction among children with moderate acute malnutrition in sierra leone |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592211/ https://www.ncbi.nlm.nih.gov/pubmed/34460425 http://dx.doi.org/10.4269/ajtmh.21-0348 |
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