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Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study
CONTEXT: Circulating microRNAs (miR) have revolutionized the field of molecular biology owing to their potential as a diagnostic as well as a prognostic biomarker of cardiovascular disease and dysfunctions. The present study aims to identify the circulating miR-126 and -122 as an independent risk pr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Communications and Publications Division (CPD) of the IFCC
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592629/ https://www.ncbi.nlm.nih.gov/pubmed/34819824 |
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author | Ali, Wahid Mishra, Sridhar Rizvi, Aliya Pradhan, Akshaaya Perrone, Marco A. |
author_facet | Ali, Wahid Mishra, Sridhar Rizvi, Aliya Pradhan, Akshaaya Perrone, Marco A. |
author_sort | Ali, Wahid |
collection | PubMed |
description | CONTEXT: Circulating microRNAs (miR) have revolutionized the field of molecular biology owing to their potential as a diagnostic as well as a prognostic biomarker of cardiovascular disease and dysfunctions. The present study aims to identify the circulating miR-126 and -122 as an independent risk predictors of coronary artery disease cases. METHODS AND MATERIAL: Blood samples were collected from coronary artery disease cases (n=100) and non-CAD cases (n=100). Serum RNA was isolated by Trizol method. MiR levels were measured by quantitative real-time polymerase chain reaction with the specific primer probe set. RESULTS: MiR-126 levels were significantly down-regulated in CAD cases compared to non-CAD cases (controls) (80.0% vs. 39.0%, χ(2)=14.95, p<0.001). The level of miR-122 was significantly up-regulated in CAD cases in comparison to its non-CAD variant (14.0% vs. 63.0%, χ(2)=21.23, p<0.001). Multivariate analysis found chest pain (OR=37.07, 95% CI=3.21-169.04, p=0.017) and miR-126 (OR=0.01, 95% CI=0.00-0.63, p=0.030) as independent risk predictors of CAD. CONCLUSION: The results of our study show the potential of circulating miR-126 as a novel non-invasive biomarker in the risk prediction of CAD. Further unraveling of the role of miR-122 and miR-126 in the pathogenesis and progression of CAD will add to our understanding of the disease process leading to a new diagnostic approach. HIGHLIGHTS: Mir-122 and -126 significantly differentiate non CAD cases from angiographically proven CAD cases. Chest pain and miR-126 might work as an independent risk predictor of coronary artery disease. |
format | Online Article Text |
id | pubmed-8592629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Communications and Publications Division (CPD) of the IFCC |
record_format | MEDLINE/PubMed |
spelling | pubmed-85926292021-11-23 Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study Ali, Wahid Mishra, Sridhar Rizvi, Aliya Pradhan, Akshaaya Perrone, Marco A. EJIFCC Research Article CONTEXT: Circulating microRNAs (miR) have revolutionized the field of molecular biology owing to their potential as a diagnostic as well as a prognostic biomarker of cardiovascular disease and dysfunctions. The present study aims to identify the circulating miR-126 and -122 as an independent risk predictors of coronary artery disease cases. METHODS AND MATERIAL: Blood samples were collected from coronary artery disease cases (n=100) and non-CAD cases (n=100). Serum RNA was isolated by Trizol method. MiR levels were measured by quantitative real-time polymerase chain reaction with the specific primer probe set. RESULTS: MiR-126 levels were significantly down-regulated in CAD cases compared to non-CAD cases (controls) (80.0% vs. 39.0%, χ(2)=14.95, p<0.001). The level of miR-122 was significantly up-regulated in CAD cases in comparison to its non-CAD variant (14.0% vs. 63.0%, χ(2)=21.23, p<0.001). Multivariate analysis found chest pain (OR=37.07, 95% CI=3.21-169.04, p=0.017) and miR-126 (OR=0.01, 95% CI=0.00-0.63, p=0.030) as independent risk predictors of CAD. CONCLUSION: The results of our study show the potential of circulating miR-126 as a novel non-invasive biomarker in the risk prediction of CAD. Further unraveling of the role of miR-122 and miR-126 in the pathogenesis and progression of CAD will add to our understanding of the disease process leading to a new diagnostic approach. HIGHLIGHTS: Mir-122 and -126 significantly differentiate non CAD cases from angiographically proven CAD cases. Chest pain and miR-126 might work as an independent risk predictor of coronary artery disease. The Communications and Publications Division (CPD) of the IFCC 2021-10-18 /pmc/articles/PMC8592629/ /pubmed/34819824 Text en Copyright © 2021 International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This is a Platinum Open Access Journal distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ali, Wahid Mishra, Sridhar Rizvi, Aliya Pradhan, Akshaaya Perrone, Marco A. Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study |
title | Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study |
title_full | Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study |
title_fullStr | Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study |
title_full_unstemmed | Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study |
title_short | Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study |
title_sort | circulating microrna-126 as an independent risk predictor of coronary artery disease: a case-control study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592629/ https://www.ncbi.nlm.nih.gov/pubmed/34819824 |
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