Cargando…

Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study

CONTEXT: Circulating microRNAs (miR) have revolutionized the field of molecular biology owing to their potential as a diagnostic as well as a prognostic biomarker of cardiovascular disease and dysfunctions. The present study aims to identify the circulating miR-126 and -122 as an independent risk pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Ali, Wahid, Mishra, Sridhar, Rizvi, Aliya, Pradhan, Akshaaya, Perrone, Marco A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Communications and Publications Division (CPD) of the IFCC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592629/
https://www.ncbi.nlm.nih.gov/pubmed/34819824
_version_ 1784599504743301120
author Ali, Wahid
Mishra, Sridhar
Rizvi, Aliya
Pradhan, Akshaaya
Perrone, Marco A.
author_facet Ali, Wahid
Mishra, Sridhar
Rizvi, Aliya
Pradhan, Akshaaya
Perrone, Marco A.
author_sort Ali, Wahid
collection PubMed
description CONTEXT: Circulating microRNAs (miR) have revolutionized the field of molecular biology owing to their potential as a diagnostic as well as a prognostic biomarker of cardiovascular disease and dysfunctions. The present study aims to identify the circulating miR-126 and -122 as an independent risk predictors of coronary artery disease cases. METHODS AND MATERIAL: Blood samples were collected from coronary artery disease cases (n=100) and non-CAD cases (n=100). Serum RNA was isolated by Trizol method. MiR levels were measured by quantitative real-time polymerase chain reaction with the specific primer probe set. RESULTS: MiR-126 levels were significantly down-regulated in CAD cases compared to non-CAD cases (controls) (80.0% vs. 39.0%, χ(2)=14.95, p<0.001). The level of miR-122 was significantly up-regulated in CAD cases in comparison to its non-CAD variant (14.0% vs. 63.0%, χ(2)=21.23, p<0.001). Multivariate analysis found chest pain (OR=37.07, 95% CI=3.21-169.04, p=0.017) and miR-126 (OR=0.01, 95% CI=0.00-0.63, p=0.030) as independent risk predictors of CAD. CONCLUSION: The results of our study show the potential of circulating miR-126 as a novel non-invasive biomarker in the risk prediction of CAD. Further unraveling of the role of miR-122 and miR-126 in the pathogenesis and progression of CAD will add to our understanding of the disease process leading to a new diagnostic approach. HIGHLIGHTS: Mir-122 and -126 significantly differentiate non CAD cases from angiographically proven CAD cases. Chest pain and miR-126 might work as an independent risk predictor of coronary artery disease.
format Online
Article
Text
id pubmed-8592629
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Communications and Publications Division (CPD) of the IFCC
record_format MEDLINE/PubMed
spelling pubmed-85926292021-11-23 Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study Ali, Wahid Mishra, Sridhar Rizvi, Aliya Pradhan, Akshaaya Perrone, Marco A. EJIFCC Research Article CONTEXT: Circulating microRNAs (miR) have revolutionized the field of molecular biology owing to their potential as a diagnostic as well as a prognostic biomarker of cardiovascular disease and dysfunctions. The present study aims to identify the circulating miR-126 and -122 as an independent risk predictors of coronary artery disease cases. METHODS AND MATERIAL: Blood samples were collected from coronary artery disease cases (n=100) and non-CAD cases (n=100). Serum RNA was isolated by Trizol method. MiR levels were measured by quantitative real-time polymerase chain reaction with the specific primer probe set. RESULTS: MiR-126 levels were significantly down-regulated in CAD cases compared to non-CAD cases (controls) (80.0% vs. 39.0%, χ(2)=14.95, p<0.001). The level of miR-122 was significantly up-regulated in CAD cases in comparison to its non-CAD variant (14.0% vs. 63.0%, χ(2)=21.23, p<0.001). Multivariate analysis found chest pain (OR=37.07, 95% CI=3.21-169.04, p=0.017) and miR-126 (OR=0.01, 95% CI=0.00-0.63, p=0.030) as independent risk predictors of CAD. CONCLUSION: The results of our study show the potential of circulating miR-126 as a novel non-invasive biomarker in the risk prediction of CAD. Further unraveling of the role of miR-122 and miR-126 in the pathogenesis and progression of CAD will add to our understanding of the disease process leading to a new diagnostic approach. HIGHLIGHTS: Mir-122 and -126 significantly differentiate non CAD cases from angiographically proven CAD cases. Chest pain and miR-126 might work as an independent risk predictor of coronary artery disease. The Communications and Publications Division (CPD) of the IFCC 2021-10-18 /pmc/articles/PMC8592629/ /pubmed/34819824 Text en Copyright © 2021 International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This is a Platinum Open Access Journal distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ali, Wahid
Mishra, Sridhar
Rizvi, Aliya
Pradhan, Akshaaya
Perrone, Marco A.
Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study
title Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study
title_full Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study
title_fullStr Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study
title_full_unstemmed Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study
title_short Circulating microRNA-126 as an Independent Risk Predictor of Coronary Artery Disease: A Case-Control Study
title_sort circulating microrna-126 as an independent risk predictor of coronary artery disease: a case-control study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592629/
https://www.ncbi.nlm.nih.gov/pubmed/34819824
work_keys_str_mv AT aliwahid circulatingmicrorna126asanindependentriskpredictorofcoronaryarterydiseaseacasecontrolstudy
AT mishrasridhar circulatingmicrorna126asanindependentriskpredictorofcoronaryarterydiseaseacasecontrolstudy
AT rizvialiya circulatingmicrorna126asanindependentriskpredictorofcoronaryarterydiseaseacasecontrolstudy
AT pradhanakshaaya circulatingmicrorna126asanindependentriskpredictorofcoronaryarterydiseaseacasecontrolstudy
AT perronemarcoa circulatingmicrorna126asanindependentriskpredictorofcoronaryarterydiseaseacasecontrolstudy