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Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines

Occupational exposure to the arylamines benzidine and β-naphthylamine increase bladder cancer risk up to 100-fold, making them some of the most powerful human carcinogens. We hypothesize that tumors arising in people with occupational exposures have different patterns of gene expression than histolo...

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Autores principales: Kolli, Ramya T., Xu, Zongli, Panduri, Vijayalakshmi, Taylor, Jack A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592720/
https://www.ncbi.nlm.nih.gov/pubmed/34790817
http://dx.doi.org/10.1155/2021/2624433
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author Kolli, Ramya T.
Xu, Zongli
Panduri, Vijayalakshmi
Taylor, Jack A.
author_facet Kolli, Ramya T.
Xu, Zongli
Panduri, Vijayalakshmi
Taylor, Jack A.
author_sort Kolli, Ramya T.
collection PubMed
description Occupational exposure to the arylamines benzidine and β-naphthylamine increase bladder cancer risk up to 100-fold, making them some of the most powerful human carcinogens. We hypothesize that tumors arising in people with occupational exposures have different patterns of gene expression than histologically similar tumors from people without such exposures. In a case-case study, we compare gene expression in 22 formalin-fixed paraffin-embedded (FFPE) bladder tumors from men with high-level occupational exposure to arylamines to that in 26 FFPE bladder tumors from men without such exposure. Gene expression analysis was performed on the NanoString nCounter system using a PanCancer Progression Panel comprised of 740 cancer progression-related genes and a custom panel of 69 arylamine- and bladder cancer-related genes which were chosen from in vitro studies. Although fold differences were small, there was evidence of differential expression by exposure status for 17 genes from the Progression Panel and 4 genes from the custom panel. In total, 10 genes showed dose-response association at a p < 0.01, of which 4 genes (CD46, NR4A1, BAX, and YWHAZ) passed a false discovery rate (FDR) q value cutoff of 0.05 but were not significant after Bonferroni correction. Overall, we find limited evidence for differentially expressed genes in pathways related to DNA damage signaling and epithelial-to-mesenchymal transition (EMT).
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spelling pubmed-85927202021-11-16 Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines Kolli, Ramya T. Xu, Zongli Panduri, Vijayalakshmi Taylor, Jack A. Biomed Res Int Research Article Occupational exposure to the arylamines benzidine and β-naphthylamine increase bladder cancer risk up to 100-fold, making them some of the most powerful human carcinogens. We hypothesize that tumors arising in people with occupational exposures have different patterns of gene expression than histologically similar tumors from people without such exposures. In a case-case study, we compare gene expression in 22 formalin-fixed paraffin-embedded (FFPE) bladder tumors from men with high-level occupational exposure to arylamines to that in 26 FFPE bladder tumors from men without such exposure. Gene expression analysis was performed on the NanoString nCounter system using a PanCancer Progression Panel comprised of 740 cancer progression-related genes and a custom panel of 69 arylamine- and bladder cancer-related genes which were chosen from in vitro studies. Although fold differences were small, there was evidence of differential expression by exposure status for 17 genes from the Progression Panel and 4 genes from the custom panel. In total, 10 genes showed dose-response association at a p < 0.01, of which 4 genes (CD46, NR4A1, BAX, and YWHAZ) passed a false discovery rate (FDR) q value cutoff of 0.05 but were not significant after Bonferroni correction. Overall, we find limited evidence for differentially expressed genes in pathways related to DNA damage signaling and epithelial-to-mesenchymal transition (EMT). Hindawi 2021-11-08 /pmc/articles/PMC8592720/ /pubmed/34790817 http://dx.doi.org/10.1155/2021/2624433 Text en Copyright © 2021 Ramya T. Kolli et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kolli, Ramya T.
Xu, Zongli
Panduri, Vijayalakshmi
Taylor, Jack A.
Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_full Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_fullStr Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_full_unstemmed Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_short Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines
title_sort differential gene expression in bladder tumors from workers occupationally exposed to arylamines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592720/
https://www.ncbi.nlm.nih.gov/pubmed/34790817
http://dx.doi.org/10.1155/2021/2624433
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