Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro

We evaluated whether insulin could stimulate β-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with β-alanine (350 or 700 µmol·L(−1)), with insulin (100 µU·mL(−1)) either added to the media or not. Insulin stimulated the β-alanine u...

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Autores principales: Santos, Lívia, Gonçalves, L. S., Bagheri-Hanei, Shirin, Möller, Gabriella Berwig, Sale, Craig, James, Ruth M., Artioli, Guilherme Giannini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592947/
https://www.ncbi.nlm.nih.gov/pubmed/34676442
http://dx.doi.org/10.1007/s00726-021-03090-9
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author Santos, Lívia
Gonçalves, L. S.
Bagheri-Hanei, Shirin
Möller, Gabriella Berwig
Sale, Craig
James, Ruth M.
Artioli, Guilherme Giannini
author_facet Santos, Lívia
Gonçalves, L. S.
Bagheri-Hanei, Shirin
Möller, Gabriella Berwig
Sale, Craig
James, Ruth M.
Artioli, Guilherme Giannini
author_sort Santos, Lívia
collection PubMed
description We evaluated whether insulin could stimulate β-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with β-alanine (350 or 700 µmol·L(−1)), with insulin (100 µU·mL(−1)) either added to the media or not. Insulin stimulated the β-alanine uptake at the lower (350 µmol·L(−1)) but not higher (700 µmol·L(−1)) β-alanine concentration in culture medium, indicating that transporter saturation might blunt the stimulatory effects of insulin.
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spelling pubmed-85929472021-11-19 Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro Santos, Lívia Gonçalves, L. S. Bagheri-Hanei, Shirin Möller, Gabriella Berwig Sale, Craig James, Ruth M. Artioli, Guilherme Giannini Amino Acids Short Communication We evaluated whether insulin could stimulate β-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with β-alanine (350 or 700 µmol·L(−1)), with insulin (100 µU·mL(−1)) either added to the media or not. Insulin stimulated the β-alanine uptake at the lower (350 µmol·L(−1)) but not higher (700 µmol·L(−1)) β-alanine concentration in culture medium, indicating that transporter saturation might blunt the stimulatory effects of insulin. Springer Vienna 2021-10-21 2021 /pmc/articles/PMC8592947/ /pubmed/34676442 http://dx.doi.org/10.1007/s00726-021-03090-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Short Communication
Santos, Lívia
Gonçalves, L. S.
Bagheri-Hanei, Shirin
Möller, Gabriella Berwig
Sale, Craig
James, Ruth M.
Artioli, Guilherme Giannini
Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro
title Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro
title_full Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro
title_fullStr Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro
title_full_unstemmed Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro
title_short Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro
title_sort insulin stimulates β-alanine uptake in skeletal muscle cells in vitro
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592947/
https://www.ncbi.nlm.nih.gov/pubmed/34676442
http://dx.doi.org/10.1007/s00726-021-03090-9
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